In vitro Time-lapse Live-Cell Imaging to Explore Cell Migration toward the Organ of Corti

10.3791/61947 ◽  
2020 ◽  
Author(s):  
Jeong-Eun Park ◽  
Su Hoon Lee ◽  
Dong Jun Park ◽  
Young Joon Seo ◽  
Sung Kyun Kim
Keyword(s):  
Cell Migration ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Organ Of Corti ◽  
Time Lapse ◽  
Live Cell

scholarly journals Time-lapse lens-free imaging of cell migration in diverse physical microenvironments

Lab on a Chip ◽  
2016 ◽  
Vol 16 (17) ◽  
pp. 3304-3316 ◽  
Author(s):  
Evelien Mathieu ◽  
Colin D. Paul ◽  
Richard Stahl ◽  
Geert Vanmeerbeeck ◽  
Veerle Reumers ◽  
...  
Keyword(s):  
Cell Migration ◽  
Live Cell Imaging ◽  
Phase Contrast ◽  
Cell Imaging ◽  
Time Lapse ◽  
Live Cell ◽  
Phase Contrast Microscopy ◽  
Contrast Microscopy ◽  
Coherent Illumination

Lens-free imaging using coherent illumination is established as an inexpensive and reliable alternative to conventional phase contrast microscopy for live-cell imaging applications.

scholarly journals Time-Lapse, Live-Cell Imaging of Potassium Efflux in Cell Exposed to Nanosecond Pulsed Electric Fields

2021 ◽  
Vol 120 (3) ◽  
pp. 223a
Author(s):  
Flavia Mazzarda ◽  
Esin B. Sozer ◽  
Julia L. Pittaluga ◽  
Claudia Muratori ◽  
P. Thomas Vernier
Keyword(s):  
Electric Fields ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Pulsed Electric Fields ◽  
Time Lapse ◽  
Live Cell ◽  
Potassium Efflux ◽  
Nanosecond Pulsed Electric Fields

Dye selection for live cell imaging of intact siRNA

2012 ◽  
Vol 393 (1-2) ◽  
pp. 23-35 ◽  
Author(s):  
Markus Hirsch ◽  
Dennis Strand ◽  
Mark Helm
Keyword(s):  
Live Cell Imaging ◽  
Cell Imaging ◽  
Fluorescent Dyes ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Time Lapse ◽  
Live Cell ◽  
High Yield ◽  
Ph Variation ◽  
Rnai Efficiency

Abstract Investigations into the fate of small interfering RNA (siRNA) after transfection may unravel new ways to improve RNA interference (RNAi) efficiency. Because intracellular degradation of RNA may prevent reliable observation of fluorescence-labeled siRNA, new tools for fluorescence microscopy are warranted to cover the considerable duration of the RNAi effect. Here, the characterization and application of new fluorescence resonance energy transfer (FRET) dye pairs for sensing the integrity of duplex siRNA is reported, which allows an assessment of the degradation status of an siRNA cell population by live cell imaging. A panel of high-yield fluorescent dyes has been investigated for their suitability as FRET pairs for the investigation of RNA inside the cell. Nine dyes in 13 FRET pairs were evaluated based on the performance in assays of photostability, cross-excitation, bleed-through, as well as on quantified changes of fluorescence as a consequence of, e.g., RNA strand hybridization and pH variation. The Atto488/Atto590 FRET pair has been applied to live cell imaging, and has revealed first aspects of unusual trafficking of intact siRNA. A time-lapse study showed highly dynamic movement of siRNA in large perinuclear structures. These and the resulting optimized FRET labeled siRNA are expected to have significant impact on future observations of labeled RNAs in living cells.

A new visible-light-excitable ICT-CHEF-mediated fluorescence ‘turn-on’ probe for the selective detection of Cd2+ in a mixed aqueous system with live-cell imaging

2015 ◽  
Vol 44 (12) ◽  
pp. 5763-5770 ◽  
Author(s):  
Shyamaprosad Goswami ◽  
Krishnendu Aich ◽  
Sangita Das ◽  
Chitrangada Das Mukhopadhyay ◽  
Deblina Sarkar ◽  
...  
Keyword(s):  
Visible Light ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Aqueous System ◽  
Live Cell ◽  
Selective Detection ◽  
Turn On ◽  
Fluorescence Turn On

A new quinoline based sensor was developed and applied for the selective detection of Cd2+ both in vitro and in vivo.

scholarly journals tdLanYFP, a yellow, bright, photostable and pH insensitive fluorescent protein for live cell imaging and FRET-based sensing strategies

2021 ◽  
Author(s):  
Y. Bousmah ◽  
H. Valenta ◽  
G. Bertolin ◽  
U. Singh ◽  
V. Nicolas ◽  
...  
Keyword(s):  
Single Molecule ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Fluorescent Protein ◽  
Fluorescent Proteins ◽  
Yellow Fluorescent Protein ◽  
Resonance Energy ◽  
Live Cell ◽  
Live Cells

AbstractYellow fluorescent proteins (YFP) are widely used as optical reporters in Förster Resonance Energy Transfer (FRET) based biosensors. Although great improvements have been done, the sensitivity of the biosensors is still limited by the low photostability and the poor fluorescence performances of YFPs at acidic pHs. In fact, today, there is no yellow variant derived from the EYFP with a pK1/2 below ∼5.5. Here, we characterize a new yellow fluorescent protein, tdLanYFP, derived from the tetrameric protein from the cephalochordate B. lanceolatum, LanYFP. With a quantum yield of 0.92 and an extinction coefficient of 133 000 mol−1.L.cm−1, it is, to our knowledge, the brightest dimeric fluorescent protein available, and brighter than most of the monomeric YFPs. Contrasting with EYFP and its derivatives, tdLanYFP has a very high photostability in vitro and preserves this property in live cells. As a consequence, tdLanYFP allows the imaging of cellular structures with sub-diffraction resolution with STED nanoscopy. We also demonstrate that the combination of high brightness and strong photostability is compatible with the use of spectro-microscopies in single molecule regimes. Its very low pK1/2 of 3.9 makes tdLanYFP an excellent tag even at acidic pHs. Finally, we show that tdLanYFP can be a FRET partner either as donor or acceptor in different biosensing modalities. Altogether, these assets make tdLanYFPa very attractive yellow fluorescent protein for long-term or single-molecule live-cell imaging that is also suitable for FRET experiment including at acidic pH.

scholarly journals Time-lapse live cell imaging to monitor doxorubicin release from DNA origami nanostructures

2018 ◽  
Vol 6 (11) ◽  
pp. 1605-1612 ◽  
Author(s):  
Yun Zeng ◽  
Jiajun Liu ◽  
Shuo Yang ◽  
Wenyan Liu ◽  
Liang Xu ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Time Lapse ◽  
Dna Origami ◽  
Live Cell ◽  
Doxorubicin Release

DNA origami nanostructures can serve as a promising carrier for drug delivery due to the outstanding programmability and biocompatibility.

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