scholarly journals Evaluation of Cancer Stem Cell Migration Using Compartmentalizing Microfluidic Devices and Live Cell Imaging

10.3791/3297 ◽  
2011 ◽  
Author(s):  
Yu Huang ◽  
Basheal Agrawal ◽  
Paul A. Clark ◽  
Justin C. Williams ◽  
John S. Kuo
Keyword(s):  
Stem Cell ◽  
Cell Migration ◽  
Cancer Stem Cell ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Microfluidic Devices ◽  
Live Cell ◽  
Stem Cell Migration

Long-Term Live Cell Imaging of Breast Cancer Stem Cell Biomarkers Using Nanoparticle Labels

2015 ◽  
Author(s):  
Maria Navas-Moreno ◽  
Tatyana Chernenko ◽  
Majid Mehrpouyen ◽  
Ming Yan ◽  
Demet Candas ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Stem Cell ◽  
Cancer Stem Cell ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Live Cell ◽  
Breast Cancer Stem Cell

scholarly journals Curcumin inhibits breast cancer stem cell migration by amplifying the E-cadherin/β-catenin negative feedback loop

2014 ◽  
Vol 5 (5) ◽  
pp. 116 ◽  
Author(s):  
Shravanti Mukherjee ◽  
Minakshi Mazumdar ◽  
Samik Chakraborty ◽  
Argha Manna ◽  
Shilpi Saha ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Stem Cell ◽  
Cell Migration ◽  
Cancer Stem Cell ◽  
Negative Feedback ◽  
Feedback Loop ◽  
Breast Cancer Stem Cell ◽  
Negative Feedback Loop ◽  
Stem Cell Migration ◽  
E Cadherin

scholarly journals Microfluidics-based devices: New tools for studying cancer and cancer stem cell migration

2011 ◽  
Vol 5 (1) ◽  
pp. 013412 ◽  
Author(s):  
Yu Huang ◽  
Basheal Agrawal ◽  
Dandan Sun ◽  
John S. Kuo ◽  
Justin C. Williams
Keyword(s):  
Stem Cell ◽  
Cell Migration ◽  
Cancer Stem Cell ◽  
Stem Cell Migration

1100 REGULATION OF RADIATION INDUCED LIVER CANCER STEM CELL MIGRATION AND METASTASIS BY ADAM17

2013 ◽  
Vol 58 ◽  
pp. S449-S450
Author(s):  
S.K. Yoon ◽  
S.W. Hong ◽  
W. Hur ◽  
Y.K. Lee ◽  
S.W. Choi ◽  
...  
Keyword(s):  
Stem Cell ◽  
Cell Migration ◽  
Liver Cancer ◽  
Cancer Stem Cell ◽  
Stem Cell Migration ◽  
Radiation Induced ◽  
Liver Cancer Stem Cell

scholarly journals Ex vivo Inducing Apoptotic Mesenchymal Stem Cell by High Hydrostatic Pressure

2020 ◽  
Author(s):  
Tien Minh Le ◽  
Naoki Morimoto ◽  
Nhung Thi My Ly ◽  
Toshihito Mitsui ◽  
Sharon Claudia Notodihardjo ◽  
...  
Keyword(s):  
Stem Cell ◽  
Hydrostatic Pressure ◽  
Mesenchymal Stem Cell ◽  
High Hydrostatic Pressure ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Morphological Changes ◽  
Fluid Pressure ◽  
Live Cell ◽  
Tunel Staining

Abstract Background: Apoptosis was reported to take crucial role in mesenchymal stem cell (MSC)-mediated immunomodulation, in which apoptotic MSCs were shown to be superior compared to living MSCs. Furthermore, extracellular vesicles (Evs) derived from MSCs were revealed more specific advantages for patient safety such as lower propensity to trigger innate and adaptive immune responses. As a safety and simple operation, high hydrostatic pressure (HHP), a physical technique that uses only fluid pressure to inactivate cells or tissues, has been developed and applied in a lot of field of biosciences, including biotechnology, biomaterials, or tissue engineering. Methods: MSCs isolated from human bone marrow were suspended cultured in appropriate medium and subjected to pressurization at 50 MPa for 36 h. Then cells were collected and investigated apoptotic pathway by transmission electron microscopy (TEM), phosphatidylserine membrane translocations, cleaved caspase-3/7 and terminal deoxy-nucleotidyl transferase dUTP nick-end labeling (TUNEL) staining. Besides, viability assays and live cell imaging were also used for assessement of cell survival after pressurization. Results: We found that HHP at 50 MPa for ≥36 h completely induced MSC death by Live/Dead assay, live cell imaging and WST-8 assay up to 7 days after pressurization. The large amount of apoptotic MSCs death was found based on morphological changes in TEM, phosphatidylserine exposure, caspase activation and detection of DNA fragmentations via TUNEL staining. Conclusions: In the current study, our data revealed that HHP treatment was convenient processing which safety and effectively induced MSCs undergo apoptosis. Especially, by capable of manufacture expanding, this technique might provide numbers of manipulated products using for industrial cell-based therapies.

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