scholarly journals Carrier-free Gene Silencing by Amphiphilic Nucleic Acid Conjugates in Differentiated Intestinal Cells

2016 ◽  
Vol 5 ◽  
pp. e364 ◽  
Author(s):  
Elena Moroz ◽  
Soo Hyeon Lee ◽  
Ken Yamada ◽  
François Halloy ◽  
Saúl Martínez-Montero ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Gene Silencing ◽  
Intestinal Cells ◽  
Carrier Free ◽  
Free Gene

Cationic siRNAs Provide Carrier-Free Gene Silencing in Animal Cells

2009 ◽  
Vol 131 (49) ◽  
pp. 17730-17731 ◽  
Author(s):  
Marc Nothisen ◽  
Mitsuharu Kotera ◽  
Emilie Voirin ◽  
Jean-Serge Remy ◽  
Jean-Paul Behr
Keyword(s):  
Gene Silencing ◽  
Animal Cells ◽  
Carrier Free ◽  
Free Gene

Structure Tuning of Cationic Oligospermine–siRNA Conjugates for Carrier-Free Gene Silencing

2016 ◽  
Vol 13 (8) ◽  
pp. 2718-2728 ◽  
Author(s):  
Marc Nothisen ◽  
Jérémy Bagilet ◽  
Jean-Paul Behr ◽  
Jean-Serge Remy ◽  
Mitsuharu Kotera
Keyword(s):  
Gene Silencing ◽  
Carrier Free ◽  
Free Gene

scholarly journals Effective carrier-free gene-silencing activity of cholesterol-modified siRNAs

RSC Advances ◽  
2018 ◽  
Vol 8 (41) ◽  
pp. 22963-22966 ◽  
Author(s):  
Lidya Salim ◽  
Chris McKim ◽  
Jean-Paul Desaulniers
Keyword(s):  
Gene Silencing ◽  
Great Promise ◽  
Chemical Modifications ◽  
Pharmacokinetic Properties ◽  
Carrier Free ◽  
Free Gene ◽  
Effective Carrier

The use of short interfering RNAs (siRNAs) as therapeutics holds great promise, but chemical modifications must first be employed to improve their pharmacokinetic properties.

Carrier-Free Hybrid DNA Nanoparticles for Light-Induced Self-Delivery of Functional Nucleic Acid Enzymes

ACS Nano ◽  
2021 ◽  
Vol 15 (1) ◽  
pp. 1841-1849
Author(s):  
Leilei Shi ◽  
Wenbo Wu ◽  
Yukun Duan ◽  
Li Xu ◽  
Sha Li ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Dna Nanoparticles ◽  
Carrier Free ◽  
Hybrid Dna

RNAi gene silencing using cerasome as a viral-size siRNA-carrier free from fusion and cross-linking

2007 ◽  
Vol 17 (14) ◽  
pp. 3935-3938 ◽  
Author(s):  
Kazuki Matsui ◽  
Yoshihiro Sasaki ◽  
Takayoshi Komatsu ◽  
Masaru Mukai ◽  
Jun-ichi Kikuchi ◽  
...  
Keyword(s):  
Gene Silencing ◽  
Cross Linking ◽  
Carrier Free

scholarly journals Cationic liposome–nucleic acid complexes for gene delivery and gene silencing

2014 ◽  
Vol 38 (11) ◽  
pp. 5164-5172 ◽  
Author(s):  
Cyrus R. Safinya ◽  
Kai K. Ewert ◽  
Ramsey N. Majzoub ◽  
Cecília Leal
Keyword(s):  
Nucleic Acid ◽  
Gene Delivery ◽  
Gene Silencing ◽  
Cationic Liposome

Induction of CML28-Specific Cytotoxic T Cells by Dendritic Cells Cotransfected with CML28 Nucleic Acid Vaccination and SOCS1-Specific siRNA Expression Vector In Vitro.

Blood ◽  
2005 ◽  
Vol 106 (11) ◽  
pp. 3058-3058
Author(s):  
Donghua Zhang ◽  
Hongsheng Zhou ◽  
Lu Zhang ◽  
Yaya Wang ◽  
Min Dai ◽  
...  
Keyword(s):  
T Cells ◽  
Dendritic Cells ◽  
Nucleic Acid ◽  
Gene Silencing ◽  
Expression Vector ◽  
Cytotoxic T Cells ◽  
Target Cells ◽  
Sirna Expression ◽  
Sirna Expression Vector

Abstract CML28 is a new tumor-associated antigen overexpressed in tumor cells, which is an attractive target for antigen-specific immunotherapy. In this study, we evaluated the possibility to induce CML28-specific cytotoxic T cells by cotransfection of CML28 nucleic acid vaccination and SOCS1-specific siRNA expression vector with dendritic cells in vitro. The full length CML28 cDNA was amplified from K562 by RT-PCR and cloned into a bicistronic vector pIRES2-EGFP to construct the CML28 nucleic acid vaccination. Dendritic cells (DCs) were generated from peripheral blood mononuclear cells (PBMNCs) of HLA-A2+ healthy donor. Construct the recombinant plasmid psiRNA-hH1neo-SOCS1 encoding hairpin small interfering RNA (siRNA) targeting to suppressors of cytokine signaling 1 (SOCS1) sequences using a vector-base RNA interference technology. Cotransfect CML28 nucleic acid vaccination and recombinant siRNA vector psiRNA-hH1neo-SOCS1 into DCs by electroporation. Real-time RT-PCR was performed to validate the SCOS1 gene silencing efficacy. The expression products of CML28 nucleic acid vaccination, His-CML28 fusion protein and GFP protein, were measured by Western Blotting and fluorescence microscope respectively. Labeling autologous nonadherent fraction of PBMNCs as responder cells by 5, 6-carboxyfluorescein diacetate succinimidyl ester (CFSE) firstly, and then coculture with cotransfected DCs for mixed lymphocyte reaction (MLR). Autogous T cell proliferative response of MLR was measured by FACS by detecting CFSE. Cotransfected DCs and the PBMNCs of a HLA-A2+ primary leukemia patient were used as target cells, untransfected DCs, K562 and HL60 were used as control. The standard 51Cr-release assay was performed to measure cytotoxicity of stimulated lyphocytes. CML28 nucleic acid vaccination could encode His-CML28 and GFP protein in DCs successfully. SOCS1-specific siRNA expression vector psiRNA-hH1neo-SOCS1 significantly suppress the expression of SOCS1 in DCs. Down regulation of SOCS1 resulted in higher expression level of CD80, CD86 and CD83 in cotranfected DCs and more rounds of cell division of responder cells in CFSE-MLR, which indicates SOCS1 gene silencing greatly contribute to maturation of DCs and enhance the the proliferative response of responder cells. CTLs induced by cotransfected DCs exhibit stronger CML28-specific cytotoxicity against HLA- matched target cells comparing to CTLs induced by CML28 nucleic acid vaccination transfected DCs only. SOCS1 gene silencing in DCs could greatly enhance the anti-tumor efficacy of CML28 nucleic acid vaccination. DCs cotransfected with CML28 nucleic acid vaccination and SOCS1-specific siRNA expression vector could effectively induce autologous CML28-specific cytotoxic T cells that lyse CML28 positive tumor cells in an antigen-specific and major histocompatibility complex (MHC) class I-restricted manner.

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