Cationic siRNAs Provide Carrier-Free Gene Silencing in Animal Cells

2009 ◽  
Vol 131 (49) ◽  
pp. 17730-17731 ◽  
Author(s):  
Marc Nothisen ◽  
Mitsuharu Kotera ◽  
Emilie Voirin ◽  
Jean-Serge Remy ◽  
Jean-Paul Behr
Keyword(s):  
Gene Silencing ◽  
Animal Cells ◽  
Carrier Free ◽  
Free Gene

Structure Tuning of Cationic Oligospermine–siRNA Conjugates for Carrier-Free Gene Silencing

2016 ◽  
Vol 13 (8) ◽  
pp. 2718-2728 ◽  
Author(s):  
Marc Nothisen ◽  
Jérémy Bagilet ◽  
Jean-Paul Behr ◽  
Jean-Serge Remy ◽  
Mitsuharu Kotera
Keyword(s):  
Gene Silencing ◽  
Carrier Free ◽  
Free Gene

scholarly journals Effective carrier-free gene-silencing activity of cholesterol-modified siRNAs

RSC Advances ◽  
2018 ◽  
Vol 8 (41) ◽  
pp. 22963-22966 ◽  
Author(s):  
Lidya Salim ◽  
Chris McKim ◽  
Jean-Paul Desaulniers
Keyword(s):  
Gene Silencing ◽  
Great Promise ◽  
Chemical Modifications ◽  
Pharmacokinetic Properties ◽  
Carrier Free ◽  
Free Gene ◽  
Effective Carrier

The use of short interfering RNAs (siRNAs) as therapeutics holds great promise, but chemical modifications must first be employed to improve their pharmacokinetic properties.

scholarly journals Carrier-free Gene Silencing by Amphiphilic Nucleic Acid Conjugates in Differentiated Intestinal Cells

2016 ◽  
Vol 5 ◽  
pp. e364 ◽  
Author(s):  
Elena Moroz ◽  
Soo Hyeon Lee ◽  
Ken Yamada ◽  
François Halloy ◽  
Saúl Martínez-Montero ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Gene Silencing ◽  
Intestinal Cells ◽  
Carrier Free ◽  
Free Gene

RNAi gene silencing using cerasome as a viral-size siRNA-carrier free from fusion and cross-linking

2007 ◽  
Vol 17 (14) ◽  
pp. 3935-3938 ◽  
Author(s):  
Kazuki Matsui ◽  
Yoshihiro Sasaki ◽  
Takayoshi Komatsu ◽  
Masaru Mukai ◽  
Jun-ichi Kikuchi ◽  
...  
Keyword(s):  
Gene Silencing ◽  
Cross Linking ◽  
Carrier Free

scholarly journals Prediction Of Novel Pirna Rat Clusters Based On Mouse Pirna Clusters Using Downstream and Upstream Analysis

10.29007/zwxg ◽  
2019 ◽  
Author(s):  
Tamer Aldwairi ◽  
Federico Hoffmann ◽  
Andy Perkins
Keyword(s):  
Gene Silencing ◽  
Danio Rerio ◽  
Repetitive Elements ◽  
Sequence Conservation ◽  
Animal Cells ◽  
Novel Approach ◽  
Non Coding Rna ◽  
Genomic Clusters

PiRNAs are a particular type of small non-coding RNA. They are distinct from miRNA in size as well as other characteristics, such as the lack of sequence conservation and increased complexity when compared to their miRNA counterparts. PiRNA is considered the largest class of sRNA that is expressed especially in the animal cells. piRNAs are derived from long single-stranded RNAs, which are transcribed from genomic clusters, in contrast to other small silencing RNAs. It has been speculated that one locus could generate more than one piRNA. PiRNA corresponding to repetitive elements is fewer in mammals than in other species like Drosophila and Danio rerio, which signifies that piRNA might have possessed or gained some additional functionality in mammals. While the functionality of piRNAs may not be fully understood, they are believed to be involved in gene silencing. In this paper, we will examine a novel approach to identify potential piRNA clusters based on genes downstream and upstream location and order.

scholarly journals Carrier-free cellular uptake and the gene-silencing activity of the lipophilic siRNAs is strongly affected by the length of the linker between siRNA and lipophilic group

2011 ◽  
Vol 40 (5) ◽  
pp. 2330-2344 ◽  
Author(s):  
Natalya S. Petrova ◽  
Ivan V. Chernikov ◽  
Mariya I. Meschaninova ◽  
IIya S. Dovydenko ◽  
Aliya G. Venyaminova ◽  
...  
Keyword(s):  
Gene Silencing ◽  
Cellular Uptake ◽  
Carrier Free

scholarly journals The GW182 protein family in animal cells: New insights into domains required for miRNA-mediated gene silencing

RNA ◽  
2009 ◽  
Vol 15 (8) ◽  
pp. 1433-1442 ◽  
Author(s):  
A. Eulalio ◽  
F. Tritschler ◽  
E. Izaurralde
Keyword(s):  
Gene Silencing ◽  
Protein Family ◽  
Animal Cells

Well-Defined DNA–Polymer Miktoarm Stars for Enzyme-Resistant Nanoflares and Carrier-Free Gene Regulation

2020 ◽  
Vol 31 (3) ◽  
pp. 530-536 ◽  
Author(s):  
Hui Li ◽  
Yang Li ◽  
Yue Xiao ◽  
Bohan Zhang ◽  
Zehong Cheng ◽  
...  
Keyword(s):  
Gene Regulation ◽  
Carrier Free ◽  
Free Gene

scholarly journals The Silencing Domain of GW182 Interacts with PABPC1 To Promote Translational Repression and Degradation of MicroRNA Targets and Is Required for Target Release

2009 ◽  
Vol 29 (23) ◽  
pp. 6220-6231 ◽  
Author(s):  
Latifa Zekri ◽  
Eric Huntzinger ◽  
Susanne Heimstädt ◽  
Elisa Izaurralde
Keyword(s):  
Gene Silencing ◽  
Mirna Target ◽  
Initiation Factor ◽  
Translational Repression ◽  
Eukaryotic Initiation Factor ◽  
Animal Cells ◽  
Mirna Targets ◽  
Argonaute Proteins ◽  
Microrna Targets ◽  
Eukaryotic Initiation Factor 4G

ABSTRACT GW182 family proteins are essential in animal cells for microRNA (miRNA)-mediated gene silencing, yet the molecular mechanism that allows GW182 to promote translational repression and mRNA decay remains largely unknown. Previous studies showed that while the GW182 N-terminal domain interacts with Argonaute proteins, translational repression and degradation of miRNA targets are promoted by a bipartite silencing domain comprising the GW182 middle and C-terminal regions. Here we show that the GW182 C-terminal region is required for GW182 to release silenced mRNPs; moreover, GW182 dissociates from miRNA targets at a step of silencing downstream of deadenylation, indicating that GW182 is required to initiate but not to maintain silencing. In addition, we show that the GW182 bipartite silencing domain competes with eukaryotic initiation factor 4G for binding to PABPC1. The GW182-PABPC1 interaction is also required for miRNA target degradation; accordingly, we observed that PABPC1 associates with components of the CCR4-NOT deadenylase complex. Finally, we show that PABPC1 overexpression suppresses the silencing of miRNA targets. We propose a model in which the GW182 silencing domain promotes translational repression, at least in part, by interfering with mRNA circularization and also recruits the deadenylase complex through the interaction with PABPC1.

scholarly journals Redundancy of the Two Dicer Genes in Transgene-Induced Posttranscriptional Gene Silencing in Neurospora crassa

2004 ◽  
Vol 24 (6) ◽  
pp. 2536-2545 ◽  
Author(s):  
Caterina Catalanotto ◽  
Massimiliano Pallotta ◽  
Paul ReFalo ◽  
Matthew S. Sachs ◽  
Laurence Vayssie ◽  
...  
Keyword(s):  
Gene Silencing ◽  
Neurospora Crassa ◽  
Mrna Degradation ◽  
Animal Cells ◽  
Rnase Iii ◽  
Posttranscriptional Gene Silencing ◽  
Guide Sequence ◽  
Mechanistic Basis ◽  
Interfering Rna ◽  
Specific Mrna

ABSTRACT RNA interference (RNAi) in animals, cosuppression in plants, and quelling in fungi are homology-dependent gene silencing mechanisms in which the introduction of either double-stranded RNA (dsRNA) or transgenes induces sequence-specific mRNA degradation. These phenomena share a common genetic and mechanistic basis. The accumulation of short interfering RNA (siRNA) molecules that guide sequence-specific mRNA degradation is a common feature in both silencing mechanisms, as is the component of the RNase complex involved in mRNA cleavage. During RNAi in animal cells, dsRNA is processed into siRNA by an RNase III enzyme called Dicer. Here we show that elimination of the activity of two Dicer-like genes by mutation in the fungus Neurospora crassa eliminates transgene-induced gene silencing (quelling) and the processing of dsRNA to an siRNA form. The two Dicer-like genes appear redundant because single mutants are quelling proficient. This first demonstration of the involvement of Dicer in gene silencing induced by transgenes supports a model by which a dsRNA produced by the activity of cellular RNA-dependent RNA polymerases on transgenic transcripts is an essential intermediate of silencing.

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