Carrier-Free Hybrid DNA Nanoparticles for Light-Induced Self-Delivery of Functional Nucleic Acid Enzymes

ACS Nano ◽  
2021 ◽  
Vol 15 (1) ◽  
pp. 1841-1849
Author(s):  
Leilei Shi ◽  
Wenbo Wu ◽  
Yukun Duan ◽  
Li Xu ◽  
Sha Li ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Dna Nanoparticles ◽  
Carrier Free ◽  
Hybrid Dna

scholarly journals Hybrid DNA/RNA nanostructures with 2′-5′ linkages

Nanoscale ◽  
2020 ◽  
Vol 12 (42) ◽  
pp. 21583-21590
Author(s):  
Arun Richard Chandrasekaran ◽  
Johnsi Mathivanan ◽  
Parisa Ebrahimi ◽  
Javier Vilcapoma ◽  
Alan A. Chen ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Double Crossover ◽  
Hybrid Dna

We report here the first instance of nucleic acid nanostructures that contain 2′-5′ linkages and characterize structures of different complexities: a simple duplex to a 4-arm junction, a double crossover (DX) motif and a tensegrity triangle motif.

scholarly journals Aptamer guided delivery of nucleic acid-based nanoparticles

2016 ◽  
Vol 2 (1) ◽  
Author(s):  
Martin Panigaj ◽  
Jakob Reiser
Keyword(s):  
Nucleic Acid ◽  
Bioactive Compounds ◽  
Targeted Delivery ◽  
Intracellular Trafficking ◽  
Clinical Applications ◽  
Dna Nanostructures ◽  
Dna Nanoparticles ◽  
Recent Developments ◽  
Self Assembled

AbstractTargeted delivery of bioactive compounds is a key part of successful therapies. In this context, nucleic acid and protein-based aptamers have been shown to bind therapeutically relevant targets including receptors. In the last decade, nucleic acid-based therapeutics coupled to aptamers have emerged as a viable strategy for cell specific delivery. Additionally, recent developments in nucleic acid nanotechnology offer an abundance of possibilities to rationally design aptamer targeted RNA or DNA nanoparticles involving combinatorial use of various intrinsic functionalities. Although a host of issues including stability, safety and intracellular trafficking remain to be addressed, aptamers as simple functional chimeras or as parts of multifunctional self-assembled RNA/DNA nanostructures hold great potential for clinical applications.

Self-assembly of DNA nanoparticles through multiple catalyzed hairpin assembly for enzyme-free nucleic acid amplified detection

Talanta ◽  
2018 ◽  
Vol 179 ◽  
pp. 641-645 ◽  
Author(s):  
Hongfei He ◽  
Jianyuan Dai ◽  
Yan Meng ◽  
Zhijuan Duan ◽  
Cuisong Zhou ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Self Assembly ◽  
Dna Nanoparticles ◽  
Catalyzed Hairpin Assembly

scholarly journals Coarse-grained nucleic acid–protein model for hybrid nanotechnology

Soft Matter ◽  
2021 ◽  
Author(s):  
Jonah Procyk ◽  
Erik Poppleton ◽  
Petr Šulc
Keyword(s):  
Nucleic Acid ◽  
Coarse Grained ◽  
Nanoscale Structures ◽  
Analysis And Design ◽  
Coarse Grained Model ◽  
Protein Model ◽  
Acid Protein ◽  
Hybrid Nanotechnology ◽  
Hybrid Dna

A coarse-grained model for analysis and design of hybrid DNA-protein nanoscale structures.

scholarly journals Carrier-free Gene Silencing by Amphiphilic Nucleic Acid Conjugates in Differentiated Intestinal Cells

2016 ◽  
Vol 5 ◽  
pp. e364 ◽  
Author(s):  
Elena Moroz ◽  
Soo Hyeon Lee ◽  
Ken Yamada ◽  
François Halloy ◽  
Saúl Martínez-Montero ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Gene Silencing ◽  
Intestinal Cells ◽  
Carrier Free ◽  
Free Gene

Introduction

1978 ◽  
Vol 36 (3) ◽  
pp. 543-544
Author(s):  
W. Bernard
Keyword(s):  
Molecular Weight ◽  
Electron Microscope ◽  
Nucleic Acid ◽  
Gene Mapping ◽  
Molecular Genetics ◽  
Cell Biology ◽  
Gene Activity ◽  
Close Connection ◽  
Basic Information ◽  
Simple Systems

In comparison to many other fields of ultrastructural research in Cell Biology, the successful exploration of genes and gene activity with the electron microscope in higher organisms is a late conquest. Nucleic acid molecules of Prokaryotes could be successfully visualized already since the early sixties, thanks to the Kleinschmidt spreading technique - and much basic information was obtained concerning the shape, length, molecular weight of viral, mitochondrial and chloroplast nucleic acid. Later, additonal methods revealed denaturation profiles, distinction between single and double strandedness and the use of heteroduplexes-led to gene mapping of relatively simple systems carried out in close connection with other methods of molecular genetics.

Infection of Escherichia coli with bacteriophages

1969 ◽  
Vol 27 ◽  
pp. 370-371
Author(s):  
Manfred E. Bayer
Keyword(s):  
Escherichia Coli ◽  
Nucleic Acid ◽  
Cell Surface ◽  
Virus Type ◽  
Infection Process ◽  
Adsorption Site ◽  
The Other ◽  
Specific Receptors ◽  
Bacterial Receptors

The first step in the infection of a bacterium by a virus consists of a collision between cell and bacteriophage. The presence of virus-specific receptors on the cell surface will trigger a number of events leading eventually to release of the phage nucleic acid. The execution of the various "steps" in the infection process varies from one virus-type to the other, depending on the anatomy of the virus. Small viruses like ØX 174 and MS2 adsorb directly with their capsid to the bacterial receptors, while other phages possess attachment organelles of varying complexity. In bacteriophages T3 (Fig. 1) and T7 the small conical processes of their heads point toward the adsorption site; a welldefined baseplate is attached to the head of P22; heads without baseplates are not infective.

Nucleic Acid Molecules Prepared by Monolayer Techniques

1972 ◽  
Vol 30 ◽  
pp. 178-179
Author(s):  
Dimitrij Lang
Keyword(s):  
Electron Microscopy ◽  
Standard Deviation ◽  
Nucleic Acid ◽  
Cytochrome C ◽  
Nucleic Acids ◽  
Contour Length ◽  
Sample Standard Deviation ◽  
Molecular Weights ◽  
Length Measurements ◽  
Protein Monolayer

The success of the protein monolayer technique for electron microscopy of individual DNA molecules is based on the prevention of aggregation and orientation of the molecules during drying on specimen grids. DNA adsorbs first to a surface-denatured, insoluble cytochrome c monolayer which is then transferred to grids, without major distortion, by touching. Fig. 1 shows three basic procedures which, modified or not, permit the study of various important properties of nucleic acids, either in concert with other methods or exclusively:1) Molecular weights relative to DNA standards as well as number distributions of molecular weights can be obtained from contour length measurements with a sample standard deviation between 1 and 4%.

Snapshot blotting: The transfer of nucleic acids and nucleoprotein complexes from electrophoresis gels to EM grids

1992 ◽  
Vol 50 (1) ◽  
pp. 762-763
Author(s):  
Stephen D. Jett
Keyword(s):  
Nucleic Acid ◽  
Nucleic Acids ◽  
Nucleic Acid Binding ◽  
Mobility Shift ◽  
Carbon Coated ◽  
Nucleoprotein Complexes ◽  
Mobility Shift Assay ◽  
Protein Nucleic Acid ◽  
Gel Mobility Shift ◽  
Nucleic Acid Interactions

The electrophoresis gel mobility shift assay is a popular method for the study of protein-nucleic acid interactions. The binding of proteins to DNA is characterized by a reduction in the electrophoretic mobility of the nucleic acid. Binding affinity, stoichiometry, and kinetics can be obtained from such assays; however, it is often desirable to image the various species in the gel bands using TEM. Present methods for isolation of nucleoproteins from gel bands are inefficient and often destroy the native structure of the complexes. We have developed a technique, called “snapshot blotting,” by which nucleic acids and nucleoprotein complexes in electrophoresis gels can be electrophoretically transferred directly onto carbon-coated grids for TEM imaging.

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