Binding of EDTA, Histidine and Acetylsalicylic Acid to Zinc–Protein Complex in Intestinal Content, Intestinal Mucosa and Blood Plasma

Nature ◽  
1972 ◽  
Vol 236 (5344) ◽  
pp. 230-232 ◽  
Author(s):  
F. A. SUSO ◽  
H. M. EDWARDS
Keyword(s):  
Acetylsalicylic Acid ◽  
Blood Plasma ◽  
Intestinal Mucosa ◽  
Protein Complex ◽  
Intestinal Content ◽  
Zinc Protein

scholarly journals Analysis of Pharmacokinetic Parameters of Acetylsalicylic Acid for Prediction of Potential Nephrotoxic Effects

2021 ◽  
Vol 9 (4) ◽  
pp. 209-215
Author(s):  
L. M. Krasnykh ◽  
O. A. Goroshko ◽  
G. F. Vasilenko ◽  
G. I. Gorodetskaya ◽  
V. V. Smirnov ◽  
...  
Keyword(s):  
Salicylic Acid ◽  
Acetylsalicylic Acid ◽  
Blood Plasma ◽  
Maximum Concentration ◽  
Test Procedure ◽  
Elimination Rate ◽  
Statistical Processing ◽  
Apparent Volume ◽  
Pharmacokinetic Parameters ◽  
In Blood Plasma

Nonsteroidal anti-inflammatory drugs, including acetylsalicylic acid, can have a dose-dependent nephrotoxic effect. The study of the pharmacokinetics of acetylsalicylic acid products will contribute to timely detection and correction of side effects caused by this medicinal product.The aim of the study was to evaluate potential nephrotoxic effects following a single oral administration of 75 mg of acetylsalicylic acid, based on the analysis of the pharmacokinetic parameters.Materials and methods: the study involved 24 healthy volunteers who received 75 mg of acetylsalicylic acid (tablets) once orally. The measurement of the active metabolite of acetylsalicylic acid—salicylic acid—in blood plasma was performed by HPLC/MS using an Agilent 1200 liquid chromatography system coupled to an Agilent 6140 tandem mass spectrometer. Agilent Eclipse XDB-C18 column (4.6 mm×150 mm; 5.0 μm) was used for chromatographic separation. The test procedure used in the study was validated. The results obtained were used to calculate the pharmacokinetic parameters: Cmax (maximum concentration), Tmax (time to maximum concentration), T1/2 (half-life of the drug), AUC0-t (area under the pharmacokinetic curve from 0 to the last time point of the curve), AUC0-∞ (total area under the pharmacokinetic curve from 0 to ∞), MRT (mean residence time of the drug in the blood), Kel (elimination rate constant), Cl/F (total clearance), Vd/F (apparent volume of distribution). The Statistics (22.0.0.0) software was used for statistical processing of the results.Results: T1/2 of salicylic acid in blood plasma was determined to be 1.6 ± 0.5 h, Cmax was 4523.0 ± 725.0 ng/mL, and Tmax was 0.98 ± 0.4 h. AUC0–t was equal to 16183.0 ± 3823.0 ng×h/m, Vd/F was 12.0 ± 3.1 L/kg, and MRT was 2.9 ± 0.6 h.Conclusions: the analysis of the pharmacokinetic parameters demonstrated a high absorption rate, intensive distribution, and moderate elimination rate of salicylic acid (the main metabolite of acetylsalicylic acid), indicating a low risk of nephrotoxic effects associated with the studied dose of the drug.

Bacillus subtilis α-amylase, a zinc-protein complex

1960 ◽  
Vol 39 (2) ◽  
pp. 287-296 ◽  
Author(s):  
Eric A. Stein ◽  
Edmond H. Fischer
Keyword(s):  
Bacillus Subtilis ◽  
Protein Complex ◽  
Zinc Protein

scholarly journals THE MULTIPLICATION OF THE VIRUS OF MEXICAN TYPHUS FEVER IN FLEAS

1932 ◽  
Vol 55 (2) ◽  
pp. 307-323 ◽  
Author(s):  
H. Mooser ◽  
M. Ruiz Castaneda
Keyword(s):  
Epithelial Cells ◽  
Intestinal Mucosa ◽  
Intestinal Content ◽  
Malpighian Tubules ◽  
Peritrophic Membrane ◽  
Xenopsylla Cheopis ◽  
Typhus Fever ◽  
Entire Stomach ◽  
Held Back

The virus of Mexican typhus has been shown to multiply abundantly in the following species of fleas: Xenopsylla cheopis, Ceratophyllus fasciatus, Leptopsylla musculi, Ctenocephalus canis, Ctenocephalus felis. In all fleas, Rickettsia prowazeki was demonstrated within the epithelial cells of the stomach and within the cells of the Malpighian tubules. Whereas in infected lice enormous numbers of these organisms are discharged from the disintegrating cells into the intestinal content, only few Rickettsiae are found in the lumen of the fleas' intestines. They are held back by the peritrophic membrane, which covers the mucosa of the entire stomach. Rickettsiae seem to enter the lumen of the gut almost exclusively by the route of the Malpighian tubules. Observations were made which seem to indicate that the fleas recover from the infection and that they are able to regenerate the partly destroyed intestinal mucosa. An explanation is given for the relative harmlessness of fleas as vectors of typhus.

A New Quantum Calibrated Force Field for Zinc–Protein Complex

2013 ◽  
Vol 9 (3) ◽  
pp. 1788-1798 ◽  
Author(s):  
Tong Zhu ◽  
Xudong Xiao ◽  
Changge Ji ◽  
John Z. H. Zhang
Keyword(s):  
Force Field ◽  
Protein Complex ◽  
Zinc Protein

scholarly journals Gut Microbiota Comparison Between Intestinal Contents and Mucosa in Mice With Repeated Stress-Related Diarrhea Provides Novel Insight

2021 ◽  
Vol 12 ◽  
Author(s):  
Chen-Yang Zhang ◽  
Xin-Xin Peng ◽  
Hao-Qing Shao ◽  
Xiao-Ya Li ◽  
Yi Wu ◽  
...  
Keyword(s):  
Intestinal Mucosa ◽  
Function Analysis ◽  
Bacterial Species ◽  
Species Abundance ◽  
Intestinal Content ◽  
Repeated Stress ◽  
Intestinal Contents ◽  
Bowel Disorders ◽  
The Difference ◽  
Community Systems

Repeated stress-related diarrhea is a kind of functional bowel disorders (FBDs) that are mainly stemming from dysregulation of the microbiota–gut–brain axis mediated by a complex interplay of 5-hydroxytryptophan (5-HT). Intestinal content and intestinal mucosa microbiota belong to two different community systems, and the role of the two microbiota community systems in repeated stress-related diarrhea remains largely unknown. In order to ascertain the difference in composition and the potential function between intestinal content and intestinal mucosa microbiota response on repeated stress-related diarrhea, we collected intestinal contents and mucosa of mice with repeated stress-related diarrhea for 16S rRNA PacBio SMRT gene full-length sequencing, and with the digital modeling method of bacterial species abundance, the correlations among the two microbiota community systems and serum 5-HT concentration were analyzed. We found that the microbiotal composition differences both in intestinal contents and mucosa were consistent throughout all the phylogenetic ranks, with an increasing level of resolution. Compared with intestinal content microbiota, the diversity and composition of microbiota colonized in intestinal mucosa are more sensitive to repeated stress-related diarrhea. The PICRUSt2 of metagenomic function analysis found that repeated stress-related diarrhea is more likely to perturb the intestinal mucosa microbiota metagenomic functions involved in the neural response. We further found that the mucosal microbiota-based relative abundance model was more predictive on serum 5-HT concentration with the methods of machine-learning model established and multivariate dimensionality reduction (R2 = 0.876). These findings suggest that the intestinal mucosa microbiota might serve as a novel potential prediction model for the serum 5-HT concentration involvement in the repeated stress-related diarrhea, in addition to focusing on its mechanism in the gastrointestinal dysfunction.

Cyclic AMP in macrophages, intestinal mucosa, and blood plasma of germfree and ordinary animals

1980 ◽  
Vol 90 (1) ◽  
pp. 878-880 ◽  
Author(s):  
G. I. Podoprigora ◽  
J. Hoffman ◽  
J. Janeček ◽  
J. Naprstka
Keyword(s):  
Cyclic Amp ◽  
Blood Plasma ◽  
Intestinal Mucosa
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