Fluorescent Complement Fixation Technique

Nature ◽  
1966 ◽  
Vol 212 (5060) ◽  
pp. 418-419 ◽  
Author(s):  
T. MATUHASI ◽  
MITSUKO USUI
Keyword(s):  
Complement Fixation ◽  
Fixation Technique

CHEMISTRY OF GONADOTROPHINS IN RELATION TO THEIR ANTIGENIC PROPERTIES

1969 ◽  
Vol 62 (1_Suppl) ◽  
pp. S13-S30 ◽  
Author(s):  
W. R. Butt
Keyword(s):  
Biological Activity ◽  
Guinea Pigs ◽  
Complement Fixation ◽  
Neuraminic Acid ◽  
Fixation Technique ◽  
Immunological Activity ◽  
Antigenic Properties ◽  
Specific Antisera ◽  
Structural Differences

ABSTRACT Several chemical differences between FSH, LH and HCG have been reported: thus LH and HCG are richer in proline than FSH and FSH and HCG contain more N-acetyl neuraminic acid than LH. Sub-units of LH are formed by treatment with urea, guanidine or acid. HCG also may contain two sub-units. The sub-units from LH are biologically inert but retain their immunological activity: biological activity is restored when the sub-units are incubated together. There is much evidence from chemical and enzymic reactions that antigenic groups are distinct from those parts of the molecule essential for biological activity. N-acetyl neuraminic acid and probably other carbohydrates in FSH and HCG are not involved in immunological activity but are necessary for biological activity. Histidine, methionine and possibly cysteine appear to be essential for biological but not immunological activity of FSH, while tryptophan and possibly tyrosine are not essential for either. A few highly specific antisera to gonadotrophins have been prepared in rabbits and guinea pigs to crude antigens: there is no evidence that purified antigens are more likely to produce specific antisera. Differences in the immunological reactivities of urinary compared with pituitary gonadotrophins have been observed both by radioimmunoassay and by the complement fixation technique. The latter may be particularly useful for detecting structural differences in the hormones.

An Immunological Perspective on Evolutionary Relationships in Australian Frogs of the Hylid Genus Cyclorana

1985 ◽  
Vol 33 (1) ◽  
pp. 17 ◽  
Author(s):  
LR Maxson ◽  
DP Ondrula ◽  
MJ Tyler
Keyword(s):  
Complement Fixation ◽  
Species Group ◽  
Evolutionary Relationships ◽  
Species Relationships ◽  
Fixation Technique ◽  
Serum Albumins ◽  
Morphological Studies ◽  
Biochemical Analyses

Detailed morphological studies as well as recent biochemical analyses have demonstrated substantial differentiation within the widespread Australian hylid frog genus Cyclorana. To further investigate species relationships within Cyclorana, supplemental immunological studies were performed. Serum albumins of 10 species of Cyclorana and three species of the related hylid genus Litoria were compared by means of the quantitative micro-complement fixation technique. This analysis suggests that there are three Cyclorana lineages: C. maini, C. cultripes, C. brevipes and C. longipes forming one cluster, C. australis clustering with L. alboguttata, and C. platycephalus. All species of Cyclorana studied, as well as L. alboguttata, are genetically closest to, but distinct from, species in the L. aurea species-group.

STUDIES ON THE ANTIGENIC STRUCTURE OF HISTOPLASMA CAPSULATUM

1957 ◽  
Vol 3 (7) ◽  
pp. 975-985 ◽  
Author(s):  
N. A. Labzoffsky ◽  
J. B. Fischer ◽  
J. J. Hamvas
Keyword(s):  
Histoplasma Capsulatum ◽  
Complement Fixation ◽  
Antigenic Structure ◽  
Fixation Technique ◽  
Chemical Methods ◽  
Physical And Chemical

Employing physical and chemical methods eight antigenic fractions were isolated from Histoplasma capsulatum as determined by complement fixation technique. Two of the fractions were found to cross-react with coccidioidal antisera, two with coccidioidal and Blastomyces antisera, one with Blastomyces antisera, while the remaining three displayed specificity by reacting with Histoplasma antisera only. Some evidence is presented to indicate that the isolated fractions are antigenically distinct.

scholarly journals COMPLEMENT FIXATION WITH THE NEUROTROPIC VIRUSES

1943 ◽  
Vol 77 (2) ◽  
pp. 139-153 ◽  
Author(s):  
Walter P. Havens ◽  
Dennis W. Watson ◽  
Robert H. Green ◽  
George I. Lavin ◽  
Joseph E. Smadel
Keyword(s):  
High Speed ◽  
Complement Fixation ◽  
Normal Serum ◽  
Fixation Technique ◽  
The West ◽  
West Nile ◽  
Neurotropic Viruses ◽  
Immunological Reactions ◽  
Cross Immunity ◽  
Western Equine Encephalitis

Antigens capable of fixing complement specifically with the appropriate antibodies have been prepared from brain tissue of hamsters and mice infected with the viruses of St. Louis, Japanese, Western, and Eastern encephalitis, and with the West Nile virus. The antigens were freed of the material which reacts with normal serum by means of centrifugation at relatively high speed. In addition, the infectivity of the preparation was destroyed by irradiation with ultraviolet light. Cross reactions were demonstrated by means of the complement-fixation technique with materials from animals infected with the viruses of Eastern and Western equine encephalitis. No relationship was detectable by this procedure between St. Louis, Japanese, and West Nile viruses. These findings emphasize the need for further investigation and correlation of the immunological reactions of the groups of neurotropic viruses, since the equine agents are apparently unrelated when studied by neutralization and cross-immunity tests while these methods provide evidence of the presence of common antigenic structures in the St. Louis, Japanese, and West Nile agents.

scholarly journals Rapid Complement Fixation Technique for Estimating Complement-Fixing Antigen Elution Profiles of Viruses from Gel Filtration Columns1

1972 ◽  
Vol 24 (1) ◽  
pp. 157-159
Author(s):  
Robert A. Cornesky ◽  
William McD. Hammon ◽  
Gladys E. Sather ◽  
Robert Atchison
Keyword(s):  
Complement Fixation ◽  
Gel Filtration ◽  
Fixation Technique

scholarly journals Influenza complement-fixation. A simple quantitative micro-method

1953 ◽  
Vol 51 (4) ◽  
pp. 492-501 ◽  
Author(s):  
G. Belyavin
Keyword(s):  
Guinea Pig ◽  
Newcastle Disease ◽  
Complement Fixation ◽  
Mouse Lung ◽  
Fixation Technique ◽  
General Application ◽  
Serological Examination

The complement-fixation technique described in this paper was designed as a simple and rapid micro-method. It is derived from the method published by Fulton & Dumbell (1949), but a number of modifications have rendered it less laborious to perform and more flexible in application. Using rabbit, human and guinea-pig antisera, this technique has been successfully applied to the serological examination of the viruses of influenza, vaccinia and Newcastle disease, and experience with other materials, such as gonococcal antigen and extracts of chorio-allantoic membrane and mouse lung, suggests that it is of general application.

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