scholarly journals Submerged Culture Production of Diphtheria Toxin

Nature ◽  
1954 ◽  
Vol 174 (4429) ◽  
pp. 557-557 ◽  
Author(s):  
F. V. LINGGOOD ◽  
A. C. MATTHEWS ◽  
S. PINFIELD ◽  
C. G. POPE ◽  
T. R. SHARLAND
1970 ◽  
Vol 16 (7) ◽  
pp. 639-639 ◽  
Author(s):  
L. A. Robb ◽  
D. W. Stainer ◽  
M. J. Scholte

The method for purifying diphtheria toxin has been improved.


1968 ◽  
Vol 14 (10) ◽  
pp. 1155-1160 ◽  
Author(s):  
D. W. Stainer ◽  
J. M. C. Corkill ◽  
M. J. Scholte

A new medium has been developed which does not contain any meat digest products and is suitable for the deep-culture production of high-potency diphtheria toxins. The main ingredient is a commercially available, tryptic digest of casein known as N.Z. Amine type A. It has been found that when the iron, calcium, and phosphate levels in the medium are carefully controlled by suitable additions of ferrous sulphate, calcium chloride, and inorganic phosphate solutions, high levels of diphtheria toxin are regularly produced (200 Lf/ml in a 48-hour growth period).


Nature ◽  
1946 ◽  
Vol 157 (3983) ◽  
pp. 264-264
Author(s):  
F. V. LINGGOOD

The submerged culture production of ferroverdin, an iron-containing green pigment of a novel type, by a new species of Streptomyces Wak. is reported. The morphological properties of this micro-organism are described, and the optimum culture conditions for pigmentation in shake flasks and in stirred fermenters are determined. The Preparation and identification of reductive and alkali degradation products of ferroverdin are reported; on the basis of their structures it is suggested that ferroverdin is the ferrous complex of the p -vinylphenylester of 3-nitroso-4-hydroxybenzoic acid.


Nature ◽  
1955 ◽  
Vol 176 (4493) ◽  
pp. 1128-1128 ◽  
Author(s):  
F. V. LINGGOOD ◽  
A. C. MATTHEWS ◽  
S. PINFIELD ◽  
C. G. POPE ◽  
T. R. SHARLAND

2020 ◽  
Vol 333 ◽  
pp. 108797 ◽  
Author(s):  
Juan J. Román-Camacho ◽  
Inés M. Santos-Dueñas ◽  
Isidoro García-García ◽  
Jaime Moreno-García ◽  
Teresa García-Martínez ◽  
...  

Planta Medica ◽  
2011 ◽  
Vol 77 (12) ◽  
Author(s):  
LM Papaspyridi ◽  
E Topakas ◽  
N Aligiannis ◽  
P Christakopoulos ◽  
AL Skaltsounis ◽  
...  

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