Rates of Glycolysis in Human Red Cells in Relation to Energy Requirements for Cation Transport

Nature ◽  
1953 ◽  
Vol 172 (4385) ◽  
pp. 911-912 ◽  
Author(s):  
RALPH E. BERNSTEIN
Hypertension ◽  
1985 ◽  
Vol 7 (1) ◽  
pp. 132-139 ◽  
Author(s):  
N C Adragna ◽  
J L Chang ◽  
M C Morey ◽  
R S Williams

1966 ◽  
Vol 22 (1) ◽  
pp. 32-33 ◽  
Author(s):  
G. D'Amico ◽  
G. Sirchia ◽  
S. Ferrone ◽  
M. Romagnoni

A selective survey of the literature reveals at least three major anion-dependent cation transport systems, defined as Na + + Cl - , K + + Cl - and Na + + K + + Cl - respectively. In human red cells, kinetic data on the fraction of K + and Na + influx inhibitable by bumetanide are presented to indicate an Na + :K + stoichiometry of 1:2. For LK sheep red cells the large Cl - -dependent K + leak induced by swelling is shown to share many characteristics with that induced by A-ethyl maleimide (NEM) treatment. NEM has complex effects, both inhibiting and then activating Cl “-dependent K+ fluxes dependent on NEM concentration. The alloantibody anti-L can prevent the action of NEM. In human red cells NEM induces a large Cl - -dependent specific K + flux, which shows saturation kinetics. Its anion preference is Cl - > Br - > SCN - > I - > NO - > 3 MeSO - 4 . This transport pathway is not inhibited by oligomycin or SITS, although phloretin and high concentrations of furosemide and bumetanide (over 0.3 mM) do inhibit. Quinine (0.5 mM) is also an inhibitor. It is concluded that at least two distinct Cl - -dependent transport pathways for K + are inducible in mammalian red cells, although the evidence for their separation is not absolute.


1968 ◽  
Vol 20 (03/04) ◽  
pp. 384-396 ◽  
Author(s):  
G Zbinden ◽  
S Tomlin

SummaryAn in vitro system is described in which adhesion of blood platelets to washed and tannic acid-treated red cells was assayed quantitatively by microscopic observation. ADP, epinephrine and TAME produced a reversible increase in platelet adhesiveness which was antagonized by AMP. With Evans blue, polyanetholsulfonate, phthalanilide NSC 38280, thrombin and heparin at concentrations above 1-4 u/ml the increase was irreversible. The ADP-induced increase in adhesiveness was inhibited by sodium citrate, EDTA, AMP, ATP and N-ethylmaleimide. EDTA, AMP and the SH-blocker N-ethylmaleimide also reduced spontaneous platelet adhesion to red cells. No significant effects were observed with adenosine, phenprocoumon, 5-HT, phthalanilide NSC 57155, various estrogens, progestogens and fatty acids, acetylsalicylic acid and similarly acting agents, hydroxylamine, glucose and KCN. The method may be useful for the screening of thrombogenic and antithrombotic properties of drugs.


1990 ◽  
Vol 116 (1) ◽  
pp. 19-29 ◽  
Author(s):  
Pedro J. Romero ◽  
Carlos E. Ortíz ◽  
Carmelo Melitto

1982 ◽  
Vol 23 (2) ◽  
pp. 231-238 ◽  
Author(s):  
Harry L. Goldsmith ◽  
Phil Gold ◽  
Joseph Shuster ◽  
Koichi Takamura

1993 ◽  
Vol 1148 (1) ◽  
pp. 152-156 ◽  
Author(s):  
Virgilio L. Lew ◽  
Zipora Etzion ◽  
Robert M. Bookchin ◽  
Rui daCosta ◽  
Heikki Väänänen ◽  
...  

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