Effects of high hydrostatic pressure on ‘passive’ monovalent cation transport in human red cells

1986 ◽  
Vol 94 (1) ◽  
pp. 1-17 ◽  
Author(s):  
A. C. Hall ◽  
J. C. Ellory
Keyword(s):  
Hydrostatic Pressure ◽  
High Hydrostatic Pressure ◽  
Monovalent Cation ◽  
Cation Transport ◽  
Red Cells ◽  
Human Red Cells

Monovalent cation leaks in human red cells caused by single amino-acid substitutions in the transport domain of the band 3 chloride-bicarbonate exchanger, AE1

2005 ◽  
Vol 37 (11) ◽  
pp. 1258-1263 ◽  
Author(s):  
Lesley J Bruce ◽  
Hannah C Robinson ◽  
Hélène Guizouarn ◽  
Franck Borgese ◽  
Penny Harrison ◽  
...  
Keyword(s):  
Amino Acid ◽  
Monovalent Cation ◽  
Band 3 ◽  
Red Cells ◽  
Single Amino Acid ◽  
Amino Acid Substitutions ◽  
Transport Domain ◽  
Human Red Cells

scholarly journals Effect of exercise on cation transport in human red cells.

Hypertension ◽  
1985 ◽  
Vol 7 (1) ◽  
pp. 132-139 ◽  
Author(s):  
N C Adragna ◽  
J L Chang ◽  
M C Morey ◽  
R S Williams
Keyword(s):  
Cation Transport ◽  
Red Cells ◽  
Human Red Cells

Cation transport in normal human red cells treated with sulfhydryl compounds

1966 ◽  
Vol 22 (1) ◽  
pp. 32-33 ◽  
Author(s):  
G. D'Amico ◽  
G. Sirchia ◽  
S. Ferrone ◽  
M. Romagnoni
Keyword(s):  
Cation Transport ◽  
Red Cells ◽  
Normal Human ◽  
Sulfhydryl Compounds ◽  
Human Red Cells

Anion-dependent cation transport in erythrocytes

1982 ◽  
Vol 299 (1097) ◽  
pp. 483-495 ◽  
Keyword(s):  
Kinetic Data ◽  
Cation Transport ◽  
Red Cells ◽  
Transport Systems ◽  
Transport Pathway ◽  
Transport Pathways ◽  
Saturation Kinetics ◽  
High Concentrations ◽  
Dependent Transport ◽  
Human Red Cells

A selective survey of the literature reveals at least three major anion-dependent cation transport systems, defined as Na + + Cl - , K + + Cl - and Na + + K + + Cl - respectively. In human red cells, kinetic data on the fraction of K + and Na + influx inhibitable by bumetanide are presented to indicate an Na + :K + stoichiometry of 1:2. For LK sheep red cells the large Cl - -dependent K + leak induced by swelling is shown to share many characteristics with that induced by A-ethyl maleimide (NEM) treatment. NEM has complex effects, both inhibiting and then activating Cl “-dependent K+ fluxes dependent on NEM concentration. The alloantibody anti-L can prevent the action of NEM. In human red cells NEM induces a large Cl - -dependent specific K + flux, which shows saturation kinetics. Its anion preference is Cl - > Br - > SCN - > I - > NO - > 3 MeSO - 4 . This transport pathway is not inhibited by oligomycin or SITS, although phloretin and high concentrations of furosemide and bumetanide (over 0.3 mM) do inhibit. Quinine (0.5 mM) is also an inhibitor. It is concluded that at least two distinct Cl - -dependent transport pathways for K + are inducible in mammalian red cells, although the evidence for their separation is not absolute.

In Vitro Assay of Platelet Adhesiveness with Washed and Tanned Human Red Cells

1968 ◽  
Vol 20 (03/04) ◽  
pp. 384-396 ◽  
Author(s):  
G Zbinden ◽  
S Tomlin
Keyword(s):  
Platelet Adhesion ◽  
Sodium Citrate ◽  
Blood Platelets ◽  
In Vitro Assay ◽  
Red Cells ◽  
Platelet Adhesiveness ◽  
Vitro Assay ◽  
In Vitro System ◽  
Human Red Cells

SummaryAn in vitro system is described in which adhesion of blood platelets to washed and tannic acid-treated red cells was assayed quantitatively by microscopic observation. ADP, epinephrine and TAME produced a reversible increase in platelet adhesiveness which was antagonized by AMP. With Evans blue, polyanetholsulfonate, phthalanilide NSC 38280, thrombin and heparin at concentrations above 1-4 u/ml the increase was irreversible. The ADP-induced increase in adhesiveness was inhibited by sodium citrate, EDTA, AMP, ATP and N-ethylmaleimide. EDTA, AMP and the SH-blocker N-ethylmaleimide also reduced spontaneous platelet adhesion to red cells. No significant effects were observed with adenosine, phenprocoumon, 5-HT, phthalanilide NSC 57155, various estrogens, progestogens and fatty acids, acetylsalicylic acid and similarly acting agents, hydroxylamine, glucose and KCN. The method may be useful for the screening of thrombogenic and antithrombotic properties of drugs.

Survival of mouse blastocysts after low-temperature preservation under high pressure

2004 ◽  
Vol 52 (4) ◽  
pp. 479-487 ◽  
Author(s):  
Cs. Pribenszky ◽  
M. Molnár ◽  
S. Cseh ◽  
L. Solti
Keyword(s):  
Phase Transition ◽  
Hydrostatic Pressure ◽  
Low Temperature ◽  
High Hydrostatic Pressure ◽  
Room Temperature ◽  
Freezing Point ◽  
Significant Loss ◽  
Embryo Cryopreservation ◽  
Subzero Temperatures ◽  
Survival Capacity

Cryoinjuries are almost inevitable during the freezing of embryos. The present study examines the possibility of using high hydrostatic pressure to reduce substantially the freezing point of the embryo-holding solution, in order to preserve embryos at subzero temperatures, thus avoiding all the disadvantages of freezing. The pressure of 210 MPa lowers the phase transition temperature of water to -21°C. According to the results of this study, embryos can survive in high hydrostatic pressure environment at room temperature; the time embryos spend under pressure without significant loss in their survival could be lengthened by gradual decompression. Pressurisation at 0°C significantly reduced the survival capacity of the embryos; gradual decompression had no beneficial effect on survival at that stage. Based on the findings, the use of the phenomena is not applicable in this form, since pressure and low temperature together proved to be lethal to the embryos in these experiments. The application of hydrostatic pressure in embryo cryopreservation requires more detailed research, although the experience gained in this study can be applied usefully in different circumstances.

Sign in / Sign up

Export Citation Format

Share Document