ATPASE ERYTHROCYTE TRANSPORT SYSTEMS IN HUMAN BLOOD

Aim. The article deals with establishing the features of ATPase erythrocytes in the athletes of the first and second categories and non-athletes from different age groups. Material and methods. We studied the capillary blood of track-and-field athletes and healthy non-athletes of the same age and anthropometric characteristics (n = 60). Blood analysis was performed with the help of the Dixion hematological analyzer (Russia). The activity of erythrocyte membrane ATPases was studied according to K.S. Keeton. Results. The study revealed significant (p < 0.05) differences in certain (HGB and RBC) hematological blood indicators in participants from different age groups depending on their physical fitness. Age differences between groups are not significant (p > 0.05). The results obtained demonstrate the significantly (p < 0.001) higher values of ATPase activity in the athletes of the first and second categories from various age groups. The increase in Mg2+, Na+, K+-, Mg2+- and Na+, K+-ATPases activity in athletes is connected with more intensive metabolism in this group because transport ATPases participate in the energy supply of training and competition loads. Age-related differences (p < 0.05) in the activity of erythrocyte membrane ATPases in both groups can be possibly connected with the conformation features of erythrocyte membrane protein. The two-factor dispersion analysis revealed that age significantly (Р < 0.001) determined Mg2+, Na+, K+-, Mg2+- and Na+, K+-ATPases erythrocyte membrane activity at 89.15, 87.46, and 81.40 % respectively; physical activity at 96.19, 95.45 and 93.80 % respectively. Conclusion. General physical fitness and age can be considered as the factors determining the activity of erythrocyte membrane ATPases.

The effect of subchronic supplementation with folic acid and l-arginine on homocysteine-induced seizures

The aim of the present study was to examine the effect of subchronic co-administration of folic acid (F) and l-arginine (A) on behavioural and electroencephalographic (EEG) characteristics of dl homocysteine thiolactone (H) induced seizures in adult rats. The activity of membrane ATPases in different brain regions were also investigated. Rats were treated with F, A, or vehicle for 15 days (regimen: F 5 mg/kg + A 500 mg/kg (F5A500); F 10 mg/kg + A 300 mg/kg (F10A300)). Seizures were elicited by convulsive dose of H (H, F5A500H, F10A300H) Subchronic supplementation with F and A did not affect seizure incidence, number of seizure episodes, and severity in F5A500H and F10A300H groups vs. H group. However, a tendency to increase latency and decrease the number of seizure episodes was noticed in the F10A300H group. EEG mean spectral power densities during ictal periods were significantly lower in F10A300H vs. H group. The activity of Na+/K+-ATPase and Mg2+-ATPase was significantly increased in almost all examined structures in rats treated with F and A. We can conclude that subchronic supplementation with folic acid and l-arginine has an antiepileptic effect in dl homocysteine thiolactone induced epilepsy.