Carbon Nanotubes for the Label-Free Detection of Biomarkers

ACS Nano ◽  
2013 ◽  
Vol 7 (9) ◽  
pp. 7448-7453 ◽  
Author(s):  
Alexandra M. Münzer ◽  
Zachary P. Michael ◽  
Alexander Star
Keyword(s):  
Carbon Nanotubes ◽  
Label Free ◽  
Label Free Detection ◽  
Detection Of Biomarkers

scholarly journals Detection of Biomarkers Using LSPR Substrate with Gold Nanoparticle Array

2015 ◽  
Vol 2015 ◽  
pp. 1-6 ◽  
Author(s):  
Young Min Bae ◽  
Seung Oh Jin ◽  
Insoo Kim ◽  
Ki Young Shin ◽  
Duchang Heo ◽  
...  
Keyword(s):  
Gold Nanoparticle ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Nanoparticle Array ◽  
Label Free ◽  
Short Analysis Time ◽  
Label Free Detection ◽  
Lift Off ◽  
Detection Of Biomarkers ◽  
The Cost

In the biosensing platform, label-free detection technique provides advantages such as the short analysis time and the cost-effectiveness. In this study, we showed the feasibility of the LSPR substrate with gold nanoparticle array for detecting low density lipoprotein (LDL) and high density lipoprotein (HDL) without labeling. The LSPR substrate was fabricated through the lift-off process with the anodized alumina mask, and its LSPR phenomenon was observed by measuring the optical transmission of substrate. The antibodies were immobilized on the gold nanoparticle array via the chemical binding, in which the 11-MUA was used as the linker to bind the antibodies. The binding of antibodies was confirmed by observing the shift of LSPR peak of the substrate. Finally, with the LSPR substrates with the antibodies immobilized, the detection of LDL and HDL was investigated. As a result, LDL and HDL could be detected in the clinically available concentration range, respectively.

scholarly journals Multi-spot, Label-free Detection of Biomarkers in Complex Media by Reflectionless Surfaces

2014 ◽  
Vol 87 ◽  
pp. 58-61
Author(s):  
M. Salina ◽  
F. Giavazzi ◽  
E. Ceccarello ◽  
F. Damin ◽  
M. Chiari ◽  
...  
Keyword(s):  
Label Free ◽  
Complex Media ◽  
Label Free Detection ◽  
Detection Of Biomarkers

scholarly journals Suitability of Cell-Based Label-Free Detection for Cytotoxicity Screening of Carbon Nanotubes

2013 ◽  
Vol 2013 ◽  
pp. 1-13 ◽  
Author(s):  
Claudia Meindl ◽  
Markus Absenger ◽  
Eva Roblegg ◽  
Eleonore Fröhlich
Keyword(s):  
Carbon Nanotubes ◽  
Continuous Monitoring ◽  
Label Free ◽  
Cytotoxic Effects ◽  
Label Free Detection ◽  
Automated Microscopy ◽  
Screening Assays ◽  
Surface Functionalizations ◽  
Different Diameters ◽  
20 Nm

Cytotoxicity testing of nanoparticles (NPs) by conventional screening assays is often complicated by interference. Carbon nanotubes (CNTs) are particularly difficult to assess. To test the suitability of cell-based label-free techniques for this application, a panel of CNTs with different diameters and surface functionalizations was assessed by impedance-based technique (xCELLigenceRTCA) and automated microscopy (Cell-IQ) compared to formazan bioreduction (MTS assay). For validation of the label-free systems different concentrations of ethanol and of amine (AMI) polystyrene NPs were used. CNTs were evaluated in various cell lines, but only endothelial EAhy926 cells and L929 and V79 fibroblasts could be evaluated in all systems. Polystyrene particles obtained similar results in all assays. All systems identified thin (<8 nm) CNTs as more cytotoxic than thick (>20 nm) CNTs, but detection byxCELLigencesystem was less sensitive to CNT-induced cytotoxicity. Despite advantages, such as continuous monitoring and more detailed analysis of cytotoxic effects, label-free techniques cannot be generally recommended for cytotoxicity screening of NPs.

Electrochemical Microfluidic Paper-Based Aptasensor Platform Based on a Biotin–Streptavidin System for Label-Free Detection of Biomarkers

2021 ◽  
Vol 13 (39) ◽  
pp. 46317-46324
Author(s):  
Tao Ming ◽  
Yan Cheng ◽  
Yu Xing ◽  
Jinping Luo ◽  
Gang Mao ◽  
...  
Keyword(s):  
Label Free ◽  
Label Free Detection ◽  
Detection Of Biomarkers

Label-free detection for SNP using AIE probes and carbon nanotubes

2017 ◽  
Vol 253 ◽  
pp. 92-96 ◽  
Author(s):  
Ke Ma ◽  
Hui Wang ◽  
Haolong Li ◽  
Bin Xu ◽  
Wenjing Tian
Keyword(s):  
Carbon Nanotubes ◽  
Label Free ◽  
Label Free Detection
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