scholarly journals Detection of Biomarkers Using LSPR Substrate with Gold Nanoparticle Array

2015 ◽  
Vol 2015 ◽  
pp. 1-6 ◽  
Author(s):  
Young Min Bae ◽  
Seung Oh Jin ◽  
Insoo Kim ◽  
Ki Young Shin ◽  
Duchang Heo ◽  
...  
Keyword(s):  
Gold Nanoparticle ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Nanoparticle Array ◽  
Label Free ◽  
Short Analysis Time ◽  
Label Free Detection ◽  
Lift Off ◽  
Detection Of Biomarkers ◽  
The Cost

In the biosensing platform, label-free detection technique provides advantages such as the short analysis time and the cost-effectiveness. In this study, we showed the feasibility of the LSPR substrate with gold nanoparticle array for detecting low density lipoprotein (LDL) and high density lipoprotein (HDL) without labeling. The LSPR substrate was fabricated through the lift-off process with the anodized alumina mask, and its LSPR phenomenon was observed by measuring the optical transmission of substrate. The antibodies were immobilized on the gold nanoparticle array via the chemical binding, in which the 11-MUA was used as the linker to bind the antibodies. The binding of antibodies was confirmed by observing the shift of LSPR peak of the substrate. Finally, with the LSPR substrates with the antibodies immobilized, the detection of LDL and HDL was investigated. As a result, LDL and HDL could be detected in the clinically available concentration range, respectively.

scholarly journals Sensitive and Reproducible Gold SERS Sensor Based on Interference Lithography and Electrophoretic Deposition

Sensors ◽  
2018 ◽  
Vol 18 (11) ◽  
pp. 4076 ◽  
Author(s):  
June Hwang ◽  
Minyang Yang
Keyword(s):  
Gold Nanoparticle ◽  
Electrophoretic Deposition ◽  
Large Scale ◽  
Surface Enhanced Raman Spectroscopy ◽  
Localized Surface Plasmon ◽  
Interference Lithography ◽  
Metal Nanostructures ◽  
Nanoparticle Array ◽  
Laser Ablation In Liquid ◽  
Label Free Detection

Surface-enhanced Raman spectroscopy (SERS) is a promising analytical tool due to its label-free detection ability and superior sensitivity, which enable the detection of single molecules. Since its sensitivity is highly dependent on localized surface plasmon resonance, various methods have been applied for electric field-enhanced metal nanostructures. Despite the intensive research on practical applications of SERS, fabricating a sensitive and reproducible SERS sensor using a simple and low-cost process remains a challenge. Here, we report a simple strategy to produce a large-scale gold nanoparticle array based on laser interference lithography and the electrophoretic deposition of gold nanoparticles, generated through a pulsed laser ablation in liquid process. The fabricated gold nanoparticle array produced a sensitive, reproducible SERS signal, which allowed Rhodamine 6G to be detected at a concentration as low as 10−8 M, with an enhancement factor of 1.25 × 105. This advantageous fabrication strategy is expected to enable practical SERS applications.

Gold Nanoparticle-Based Label-Free Detection of BRCA1 Mutations Utilizing DNA Ligation on DNA Microarray

2009 ◽  
Vol 9 (2) ◽  
pp. 1019-1024 ◽  
Author(s):  
Agnishwar Girigoswami ◽  
Taihua Li ◽  
Cheulhee Jung ◽  
Hyo Young Mun ◽  
Hyun Gyu Park
Keyword(s):  
Gold Nanoparticle ◽  
Dna Microarray ◽  
Label Free ◽  
Brca1 Mutations ◽  
Dna Ligation ◽  
Label Free Detection

scholarly journals Inhibition of Extracellular Cathepsin D Reduces Hepatic Lipid Accumulation and Leads to Mild Changes in Inflammationin NASH Mice

2021 ◽  
Vol 12 ◽  
Author(s):  
Tulasi Yadati ◽  
Tom Houben ◽  
Albert Bitorina ◽  
Yvonne Oligschlaeger ◽  
Marion J. Gijbels ◽  
...  
Keyword(s):  
Lipid Accumulation ◽  
Cathepsin D ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Label Free ◽  
Sprague Dawley ◽  
Knock Out ◽  
Hepatic Lipid ◽  
Metabolic Inflammation ◽  
Hepatic Lipid Accumulation

Background & AimsThe lysosomal enzyme, cathepsin D (CTSD) has been implicated in the pathogenesis of non-alcoholic steatohepatitis (NASH), a disease characterised by hepatic steatosis and inflammation. We have previously demonstrated that specific inhibition of the extracellular CTSD leads to improved metabolic features in Sprague-Dawley rats with steatosis. However, the individual roles of extracellular and intracellular CTSD in NASH are not yet known. In the current study, we evaluated the underlying mechanisms of extracellular and intracellular CTSD fractions in NASH-related metabolic inflammation using specific small-molecule inhibitors.MethodsLow-density lipoprotein receptor knock out (Ldlr-/-) mice were fed a high-fat, high cholesterol (HFC) diet for ten weeks to induce NASH. Further, to investigate the effects of CTSD inhibition, mice were injected either with an intracellular (GA-12) or extracellular (CTD-002) CTSD inhibitor or vehicle control at doses of 50 mg/kg body weight subcutaneously once in two days for ten weeks.ResultsLdlr-/- mice treated with extracellular CTSD inhibitor showed reduced hepatic lipid accumulation and an associated increase in faecal bile acid levels as compared to intracellular CTSD inhibitor-treated mice. Furthermore, in contrast to intracellular CTSD inhibition, extracellular CTSD inhibition switched the systemic immune status of the mice to an anti-inflammatory profile. In line, label-free mass spectrometry-based proteomics revealed that extra- and intracellular CTSD fractions modulate proteins belonging to distinct metabolic pathways.ConclusionWe have provided clinically translatable evidence that extracellular CTSD inhibition shows some beneficial metabolic and systemic inflammatory effects which are distinct from intracellular CTSD inhibition. Considering that intracellular CTSD inhibition is involved in essential physiological processes, specific inhibitors capable of blocking extracellular CTSD activity, can be promising and safe NASH drugs.

scholarly journals Multi-spot, Label-free Detection of Biomarkers in Complex Media by Reflectionless Surfaces

2014 ◽  
Vol 87 ◽  
pp. 58-61
Author(s):  
M. Salina ◽  
F. Giavazzi ◽  
E. Ceccarello ◽  
F. Damin ◽  
M. Chiari ◽  
...  
Keyword(s):  
Label Free ◽  
Complex Media ◽  
Label Free Detection ◽  
Detection Of Biomarkers

Electrochemical Aptasensor for Label-Free Detection of Protein Based on Gold Nanoparticle Involved Self-Assembly

2013 ◽  
Vol 310 ◽  
pp. 177-182
Author(s):  
Song Bai Zhang ◽  
Bing Jun Zhang ◽  
Qian Liu ◽  
Xia Hu ◽  
Li Ying Zheng ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Gold Nanoparticle ◽  
Self Assembly ◽  
Gold Electrode ◽  
Hot Water ◽  
Target Protein ◽  
Capture Probe ◽  
Label Free ◽  
Label Free Detection ◽  
Self Assembled

A label-free electrochemical biosensing strategy based on gold nanoparticle involved layer-by-layer self assembly for the detection of protein is proposed using platelet derived growth factor-BB dimer (PDGF-BB) as the model analyte. Utilizing the strong sulfur-Au affinity, ethanthiol and capture probe modified gold nanoparticles are self-assembled onto the surface of gold electrode successively. The aptamer probe for target protein hybridizes with the capture probe and the biosensor is fabricated. By measuring ac current voltammetry, the target protein can be sensitively detected in a linear dynamic range from 1-1000 ng/mL with a low detection limit of 0.5 ng/mL. Making use of self-assembled gold nanoparticles layer, a large amount of capture probes can be modified onto the gold electrode, supporting the high sensitivity of the proposed strategy. In addition, good reproducibility, high selectivity and stability are achieved. In particular, the biosensor can be easily regenerated by melting in hot water, making it reusable.

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