Binding Strength of Porphyrin−Gold Nanoparticle Hybrids Based on Number and Type of Linker Moieties and a Simple Method To Calculate Inner Filter Effects of Gold Nanoparticles Using Fluorescence Spectroscopy

2015 ◽  
Vol 119 (7) ◽  
pp. 1108-1116 ◽  
Author(s):  
Ahson J. Shaikh ◽  
Faiz Rabbani ◽  
Tauqir A. Sherazi ◽  
Zafar Iqbal ◽  
Sadullah Mir ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Fluorescence Spectroscopy ◽  
Gold Nanoparticle ◽  
Simple Method ◽  
Binding Strength ◽  
Inner Filter Effects ◽  
Filter Effects

Absorbance-Corrected Synchronous Fluorescence with a Fiber-Optic-Based Fluorometer

1995 ◽  
Vol 49 (7) ◽  
pp. 1041-1047 ◽  
Author(s):  
Mark A. Victor ◽  
Stanley R. Crouch
Keyword(s):  
Transfer Function ◽  
Fluorescence Spectroscopy ◽  
Fiber Optics ◽  
Fiber Optic ◽  
Coupling Efficiency ◽  
Synchronous Fluorescence ◽  
Fluorescence Measurement ◽  
Synchronous Fluorescence Spectroscopy ◽  
Inner Filter Effects ◽  
Filter Effects

Synchronous fluorescence spectroscopy is frequently used to resolve multiple components in mixtures without separation. However, uncompensated inner-filter effects can lead to unexpected and/or large quantitative errors. An instrument and a method that correct for primary and secondary inner-filter effects in synchronous fluorescence spectroscopy have been designed and characterized. A bifurcated fiber-optic-based diode array fluorometer that simultaneously measures front-surface fluorescence and absorbance was constructed. A transfer function that mathematically describes the coupling efficiency between the excitation and emission legs of the fiber optics is defined and characterized. Once characterized, such a fluorometer is capable of calculating what the fluorescence measurement would be in the absence of inner-filter effects. Several experimental procedures for determining the instrument's transfer function are compared and discussed. Applications of the various procedures to experimental fluorescence inner-filter corrections to problematic samples are demonstrated.

Self-Assembly of Gold Nanoparticle Arrays with Anodic Aluminum Oxide Template

2014 ◽  
Vol 1004-1005 ◽  
pp. 55-58
Author(s):  
Fei Wang ◽  
Hui Xu ◽  
Ying Wei Wang ◽  
Jing Yue Fang ◽  
Wei Chen ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Aluminum Oxide ◽  
Gold Nanoparticle ◽  
Anodic Aluminum Oxide ◽  
Optical Memory ◽  
Blue Shift ◽  
Memory Storage ◽  
Nanoparticle Arrays ◽  
Simple Method ◽  
Anodic Aluminum

Nanoparticles and their ordered arrays have many important applications in fields such as electronic, optical, memory storage devices and sensors. Herein, a simple method was used for fabricating three-dimensional ordered gold nanoparticle arrays with template technique. Gold films were deposited onto anodic aluminum oxide by using electron beam evaporation. After thermal annealing, interestingly, order arrays of gold nanoparticles were obtained by a thermodynamically driven self-assembled process. The spectrum taken by UV-Vis spectrophotometer demonstrates clear surface plasmon resonance at around 500 nm, which appears blue-shift compared with usual gold nanoparticles.

Correction for Inner Filter Effects in Fluorescence Spectroscopy

1983 ◽  
Vol 66 (7) ◽  
pp. 1929-1935 ◽  
Author(s):  
Hans-Peter Lutz ◽  
Pier Luigi Luisi
Keyword(s):  
Fluorescence Spectroscopy ◽  
Inner Filter Effects ◽  
Filter Effects

Gold nanoparticles supported on zirconium, tin and ruthenium oxides for reagentless electrochemical sensing of hydrogen peroxide

2015 ◽  
Vol 7 (8) ◽  
pp. 3454-3460 ◽  
Author(s):  
Venkataraman Dharuman ◽  
Chinnathambi Anjalidevi ◽  
Palinci Nagarajan Manikandan ◽  
Habibulla Imran
Keyword(s):  
Hydrogen Peroxide ◽  
Gold Nanoparticles ◽  
Metal Oxide ◽  
Gold Nanoparticle ◽  
Solid Surface ◽  
Composite Films ◽  
Electrochemical Sensing ◽  
Simple Method ◽  
Ruthenium Oxides ◽  
Supported Gold

We demonstrate a simple method for the fabrication of metal oxide (M-Ox) supported gold nanoparticle (AuNP) composite films (M-Ox–AuNP) by mechanical and electrochemical methods on solid surface for reagentless electrochemical sensing of hydrogen peroxide (H2O2).

Interference of Gold Nanoparticles with In vitro Endotoxin Detection Assays

2020 ◽  
Vol 16 (2) ◽  
pp. 204-213 ◽  
Author(s):  
Melissa A. Vetten ◽  
Mary Gulumian
Keyword(s):  
Gold Nanoparticles ◽  
Gold Nanoparticle ◽  
Surface Reactivity ◽  
In Vitro Assays ◽  
Limulus Amebocyte Lysate ◽  
Chromogenic Assay ◽  
Nanoparticle Suspensions ◽  
Endotoxin Contamination ◽  
Factor C

Background: Endotoxin-free engineered nanoparticle suspensions are imperative for their successful applications in the field of nanomedicine as well as in the investigations in their toxicity. Gold nanoparticles are known to interfere with various in vitro assays due to their optical properties and potential for surface reactivity. In vitro endotoxin testing assays are known to be susceptible to interference caused by the sample being tested. Objective: This study aimed to identify a preferred assay for the testing of endotoxin contamination in gold nanoparticle suspensions. Methods: The interference by gold nanoparticles on three assays namely, the commonly used limulus amebocyte lysate chromogenic assay, the limulus amebocyte lysate gel-clot method, and the less common recombinant Factor C (rFC) assay, was tested. Results: Possible interference could be observed with all three assays. The interference with the absorbance- based chromogenic assay could not be overcome by dilution; whilst the qualitative nature of the gel-clot assay excluded the possibility of distinguishing between a false positive result due to enhancement of the sensitivity of the assay, and genuine endotoxin contamination. However, interference with the rFC assay was easily overcome through dilution. Conclusion: The rFC assay is recommended as an option for endotoxin contamination detection in gold nanoparticle suspensions.

scholarly journals CRISPR/Cas12a-mediated gold nanoparticle aggregation for colorimetric detection of SARS-CoV-2

2021 ◽  
Author(s):  
Yiren Cao ◽  
Jinjun Wu ◽  
Bo Pang ◽  
Hongquan Zhang ◽  
X. Chris Le
Keyword(s):  
Gold Nanoparticles ◽  
Gold Nanoparticle ◽  
Colorimetric Detection ◽  
Isothermal Amplification ◽  
Nanoparticle Aggregation ◽  
Cleavage Activity ◽  
Gold Nanoparticle Aggregation

The trans-cleavage activity of the target-activated CRISPR-Cas12a liberated an RNA crosslinker from a molecular transducer, which facilitated assembly of gold nanoparticles. Integration of the molecular transducer with isothermal amplification and...

Fluorescence biosensor for Salmonella typhimurium detection in food based on nano-self-assembly of alendronic acid modified upconversion and gold nanoparticles

2021 ◽  
Author(s):  
Min Chen ◽  
Leiqing Pan ◽  
K. Tu
Keyword(s):  
Gold Nanoparticles ◽  
Salmonella Typhimurium ◽  
Gold Nanoparticle ◽  
Alendronic Acid ◽  
Self Assembly ◽  
Upconversion Nanoparticles ◽  
Fluorescent Biosensor ◽  
Fluorescence Biosensor

A simple and quick responsive fluorescent biosensor for Salmonella typhimurium detection based on the recognition of aptamer coupled with alendronic acid (ADA)@upconversion nanoparticles (UCNPs) and gold nanoparticle (AuNPs) has been...

scholarly journals Enhanced Extracellular Synthesis of Gold Nanoparticles by Soluble Extracts from Escherichia coli Transformed with Rhizobium tropici Phytochelatin Synthase Gene

Metals ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 472
Author(s):  
Qunying Yuan ◽  
Manjula Bomma ◽  
Zhigang Xiao
Keyword(s):  
Gold Nanoparticles ◽  
Gold Nanoparticle ◽  
Synthesis Method ◽  
Nanoparticle Synthesis ◽  
Morphological Properties ◽  
Phytochelatin Synthase ◽  
Rhizobium Tropici ◽  
Whole Cells ◽  
E Coli ◽  
Recombinant Cells

Phytochelatins, the enzymatic products of phytochelatin synthase, play a principal role in protecting the plants from heavy metal and metalloid toxicity due to their ability to scavenge metal ions. In the present study, we investigated the capacity of soluble intracellular extracts from E. coli cells expressing R. tropici phytochelatin synthase to synthesize gold nanoparticle. We discovered that the reaction mediated by soluble extracts from the recombinant E. coli cells had a higher yield of gold nanoparticles, compared to that from the control cells. The compositional and morphological properties of the gold nanoparticles synthesized by the intracellular extracts from recombinant cells and control cells were similar. In addition, this extracellular nanoparticle synthesis method produced purer gold nanoparticles, avoiding the isolation of nanoparticles from cellular debris when whole cells are used to synthesize nanoparticles. Our results suggested that phytochelatins can improve the efficiency of gold nanoparticle synthesis mediated by bacterial soluble intracellular extracts, and the potential of extracellular nanoparticle synthesis platform for the production of nanoparticles in large quantity and pure form is worth further investigation.

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