Assessment of inner filter effects in fluorescence spectroscopy using the dual-pathlength method: a study of the jet fuel JP-4

1999 ◽  
Author(s):  
Todd E. Pagano ◽  
Jonathan E. Kenny
Keyword(s):  
Fluorescence Spectroscopy ◽  
Jet Fuel ◽  
Inner Filter Effects ◽  
Filter Effects

Absorbance-Corrected Synchronous Fluorescence with a Fiber-Optic-Based Fluorometer

1995 ◽  
Vol 49 (7) ◽  
pp. 1041-1047 ◽  
Author(s):  
Mark A. Victor ◽  
Stanley R. Crouch
Keyword(s):  
Transfer Function ◽  
Fluorescence Spectroscopy ◽  
Fiber Optics ◽  
Fiber Optic ◽  
Coupling Efficiency ◽  
Synchronous Fluorescence ◽  
Fluorescence Measurement ◽  
Synchronous Fluorescence Spectroscopy ◽  
Inner Filter Effects ◽  
Filter Effects

Synchronous fluorescence spectroscopy is frequently used to resolve multiple components in mixtures without separation. However, uncompensated inner-filter effects can lead to unexpected and/or large quantitative errors. An instrument and a method that correct for primary and secondary inner-filter effects in synchronous fluorescence spectroscopy have been designed and characterized. A bifurcated fiber-optic-based diode array fluorometer that simultaneously measures front-surface fluorescence and absorbance was constructed. A transfer function that mathematically describes the coupling efficiency between the excitation and emission legs of the fiber optics is defined and characterized. Once characterized, such a fluorometer is capable of calculating what the fluorescence measurement would be in the absence of inner-filter effects. Several experimental procedures for determining the instrument's transfer function are compared and discussed. Applications of the various procedures to experimental fluorescence inner-filter corrections to problematic samples are demonstrated.

Correction for Inner Filter Effects in Fluorescence Spectroscopy

1983 ◽  
Vol 66 (7) ◽  
pp. 1929-1935 ◽  
Author(s):  
Hans-Peter Lutz ◽  
Pier Luigi Luisi
Keyword(s):  
Fluorescence Spectroscopy ◽  
Inner Filter Effects ◽  
Filter Effects

Absorption-Corrected Spectral Studies of the Lucigenin Chemiluminescence Reaction

1989 ◽  
Vol 43 (5) ◽  
pp. 767-771 ◽  
Author(s):  
M. Cecilia Yappert ◽  
J. D. Ingle
Keyword(s):  
Time Dependent ◽  
Spectral Studies ◽  
Luminescence Spectra ◽  
Basic Solution ◽  
Automatic Correction ◽  
Fluorescent Product ◽  
Inner Filter Effects ◽  
Emitting Species ◽  
Filter Effects ◽  
Lucigenin Chemiluminescence

With a spectrometer based on two multichannel detectors, luminescence and absorption spectra are acquired simultaneously during the reaction between lucigenin and H2O2 in a basic solution. Alterations in the fluorescence and chemiluminescence spectral contours occur during the reaction, due to time-dependent inner filter effects caused by the changing absorption of the emission radiation by reactants, intermediates, or products. The spectrometer uses the measured absorbances and appropriate equations for automatic correction of luminescence spectra for inner filter effects. The corrected spectra demonstrate that the primary fluorescent product of the reaction, N-methyl acridone, is not the primary chemiluminescent emitting species.

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