Rapid Characterization of Wheat Low Molecular Weight Glutenin Subunits by Ultraperformance Liquid Chromatography (UPLC)

2013 ◽  
Vol 61 (17) ◽  
pp. 4026-4034 ◽  
Author(s):  
Zitong Yu ◽  
Caixia Han ◽  
Xing Yan ◽  
Xiaohui Li ◽  
Guoliang Jiang ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Liquid Chromatography ◽  
Low Molecular Weight ◽  
Glutenin Subunits ◽  
Rapid Characterization

Characterization of a wheat mutant missing low-molecular-weight glutenin subunits encoded by the B-genome

2017 ◽  
Vol 73 ◽  
pp. 158-164 ◽  
Author(s):  
Jong-Yeol Lee ◽  
Chon-Sik Kang ◽  
Hye-Rang Beom ◽  
You-Ran Jang ◽  
Susan B. Altenbach ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Low Molecular Weight ◽  
Glutenin Subunits ◽  
B Genome ◽  
Wheat Mutant

scholarly journals Searching for Low Molecular Weight Seleno-Compounds in Sprouts by Mass Spectrometry

Molecules ◽  
2020 ◽  
Vol 25 (12) ◽  
pp. 2870 ◽  
Author(s):  
Eliza Kurek ◽  
Magdalena Michalska-Kacymirow ◽  
Anna Konopka ◽  
Olga Kościuczuk ◽  
Anna Tomiak ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Molecular Weight ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Analytical Techniques ◽  
Low Molecular Weight ◽  
Analytical Protocol ◽  
Icp Ms

A fit for purpose analytical protocol was designed towards searching for low molecular weight seleno-compounds in sprouts. Complementary analytical techniques were used to collect information enabling the characterization of selenium speciation. Conceiving the overall characterization of the behavior of selenium, inductively plasma optical mass spectrometry (ICP-MS) was used to determine the total selenium content in entire sprouts as well as in selected extracts or chromatographic fractions. Then, high-performance liquid chromatography combined with ICP-MS (HPLC-ICP-MS) was used to evaluate the presence of inorganic and organic seleno-compounds, with the advantages of being very sensitive towards selenium, but limited by available selenium standard compounds. Finally, ultra-high performance liquid chromatography electrospray ionization triple quadrupole mass spectrometry (UHPLC-ESI-QqQ-MS/MS) and UHPLC-ESI-Orbitrap-MS/MS were used for the confirmation of the identity of selected compounds and identification of several unknown compounds of selenium in vegetable sprouts (sunflower, onion, radish), respectively. Cultivation of plants was designed to supplement sprouts with selenium by using solutions of selenium (IV) at the concentration of 10, 20, 40, and 60 mg/L. The applied methodology allowed to justify that vegetable sprouts metabolize inorganic selenium to a number of organic derivatives, such as seleno-methylselenocysteine (SeMetSeCys), selenomethionine (SeMet), 5′-seleno-adenosine, 2,3-DHP-selenolanthionine, Se-S conjugate of cysteine-selenoglutathione, 2,3-DHP-selenocysteine-cysteine, 2,3-DHP-selenocysteine-cysteinealanine, glutathione-2,3-DHP-selenocysteine, gamma-Glu-MetSeCys or glutamyl-glycinyl-N-2,3-DHP-selenocysteine.

scholarly journals Cloning and Characterization of Low-Molecular-Weight Glutenin Subunit Alleles from Chinese Wheat Landraces (Triticum aestivumL.)

2014 ◽  
Vol 2014 ◽  
pp. 1-6 ◽  
Author(s):  
Hongqi Si ◽  
Manli Zhao ◽  
Xin Zhang ◽  
Guoliang Yao ◽  
Genlou Sun ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Disulfide Bonds ◽  
Low Molecular Weight ◽  
Processing Quality ◽  
Repetitive Domain ◽  
Glutenin Subunits ◽  
Bread Making ◽  
Wheat Landraces ◽  
Chinese Wheat

Low-molecular-weight glutenin subunits (LMW-GS) are of great importance in processing quality and participate in the formation of polymers in wheat. In this study, eight new LMW-GS alleles were isolated from Chinese wheat landraces (Triticum aestivumL.) and designated asGlu-A3-1a,Glu-A3-1b,Glu-B3-1a,Glu-B3-1b,Glu-B3-1c,Glu-D3-1a,Glu-D3-1b, andGlu-D3-1c, which were located at theGlu-A3,Glu-B3, andGlu-D3loci, respectively. Based on the proteins encoded, the number of deduced amino acids ofGlu-B3alleles was approximately 50 more than those ofGlu-A3andGlu-D3alleles. The first cysteine ofGlu-A3andGlu-D3alleles was located at the N-terminal domain, while that ofGlu-B3alleles was found in the repetitive domain, which may lead to the different functioning in forming disulfide bonds. All the eight genes were LMW-m types and the new allele ofGlu-B3-1awhich had nine cysteine residues may be the desirable LMW-GS gene for improving bread-making quality.

Mass Spectrometric Approaches for the Characterization of Low-Molecular Weight Glutenin Subunits

2011 ◽  
pp. 34-37 ◽  
Author(s):  
V. Cunsolo ◽  
S. Foti ◽  
V. Muccilli ◽  
R. Saletti ◽  
D. Lafiandra ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Mass Spectrometric ◽  
Low Molecular Weight ◽  
Glutenin Subunits
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