scholarly journals Cloning and Characterization of Low-Molecular-Weight Glutenin Subunit Alleles from Chinese Wheat Landraces (Triticum aestivumL.)

2014 ◽  
Vol 2014 ◽  
pp. 1-6 ◽  
Author(s):  
Hongqi Si ◽  
Manli Zhao ◽  
Xin Zhang ◽  
Guoliang Yao ◽  
Genlou Sun ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Disulfide Bonds ◽  
Low Molecular Weight ◽  
Processing Quality ◽  
Repetitive Domain ◽  
Glutenin Subunits ◽  
Bread Making ◽  
Wheat Landraces ◽  
Chinese Wheat

Low-molecular-weight glutenin subunits (LMW-GS) are of great importance in processing quality and participate in the formation of polymers in wheat. In this study, eight new LMW-GS alleles were isolated from Chinese wheat landraces (Triticum aestivumL.) and designated asGlu-A3-1a,Glu-A3-1b,Glu-B3-1a,Glu-B3-1b,Glu-B3-1c,Glu-D3-1a,Glu-D3-1b, andGlu-D3-1c, which were located at theGlu-A3,Glu-B3, andGlu-D3loci, respectively. Based on the proteins encoded, the number of deduced amino acids ofGlu-B3alleles was approximately 50 more than those ofGlu-A3andGlu-D3alleles. The first cysteine ofGlu-A3andGlu-D3alleles was located at the N-terminal domain, while that ofGlu-B3alleles was found in the repetitive domain, which may lead to the different functioning in forming disulfide bonds. All the eight genes were LMW-m types and the new allele ofGlu-B3-1awhich had nine cysteine residues may be the desirable LMW-GS gene for improving bread-making quality.

scholarly journals Characterization of high- and low-molecular-weight glutenin subunits from Chinese Xinjiang wheat landraces and historical varieties

2020 ◽  
Vol 57 (10) ◽  
pp. 3823-3835
Author(s):  
Shoufen Dai ◽  
Dongyang Xu ◽  
Yongliang Yan ◽  
Zhaojin Wen ◽  
Jinbo Zhang ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Low Molecular Weight ◽  
Glutenin Subunits ◽  
Wheat Landraces

Identification of low-molecular weight glutenin subunits of wheat associated with bread-making quality

2004 ◽  
Vol 123 (4) ◽  
pp. 355-360 ◽  
Author(s):  
W. Maruyama-Funatsuki ◽  
K. Takata ◽  
Z. Nishio ◽  
T. Tabiki ◽  
E. Yahata ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Low Molecular Weight ◽  
Glutenin Subunits ◽  
Bread Making

scholarly journals Evaluation of winter wheat collection in terms of HMW- and LMW-glutenin subunits

2010 ◽  
Vol 46 (Special Issue) ◽  
pp. S96-S99 ◽  
Author(s):  
J. Bradová ◽  
L. Štočková
Keyword(s):  
Molecular Weight ◽  
Winter Wheat ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Low Molecular Weight ◽  
Glutenin Subunits ◽  
Bread Making ◽  
Wheat Varieties ◽  
Lmw Glutenin Subunits ◽  
Winter Wheat Varieties

The composition of high molecular weight (HMW-GS) and low molecular weight (LMW-GS) glutenin subunits was examined in a collection of 86 Czech registered winter wheat varieties. These proteins were analyzed by sodium dodecyl sulphate polyacrylamide gel electrophoresis. An inter-varietal polymorphism of the HMW and LMW glutenin subunits was detected. Twenty-one different patterns for HMW were identified, and eighteen for the LMW-glutenins. The different alleles encoded at the six glutenin loci were determined. Three, six, and four alleles were observed, respectively at the <I>Glu-A1, Glu-</I>B1, and <I>Glu-D1 </I>loci (encoding high HMW-GS). Three, eight, and three alleles of LMW-GS were found, respectively, at the <I>Glu-A3, Glu- B3</I>, and <I>Glu-D3 </I>loci. The evaluated varieties were split into four categories of baking quality, and these variety groups were analyzed for the presence of different HMW-GS and LMW-GS alleles. While the alleles <I>Glu-B1c </I>(7+9), and <I>Glu-D1d </I>(5+10) were detected exclusively in bread wheat varieties, the alleles <I>Glu-B1d </I>(6+8), <I>Glu-D1a </I>(2+12), and <I>Glu-A3e/f </I>only occurred in those varieties that are not suitable for bread-making. &nbsp;

Characterization of a wheat mutant missing low-molecular-weight glutenin subunits encoded by the B-genome

2017 ◽  
Vol 73 ◽  
pp. 158-164 ◽  
Author(s):  
Jong-Yeol Lee ◽  
Chon-Sik Kang ◽  
Hye-Rang Beom ◽  
You-Ran Jang ◽  
Susan B. Altenbach ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Low Molecular Weight ◽  
Glutenin Subunits ◽  
B Genome ◽  
Wheat Mutant

Rapid Characterization of Wheat Low Molecular Weight Glutenin Subunits by Ultraperformance Liquid Chromatography (UPLC)

2013 ◽  
Vol 61 (17) ◽  
pp. 4026-4034 ◽  
Author(s):  
Zitong Yu ◽  
Caixia Han ◽  
Xing Yan ◽  
Xiaohui Li ◽  
Guoliang Jiang ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Liquid Chromatography ◽  
Low Molecular Weight ◽  
Glutenin Subunits ◽  
Rapid Characterization

scholarly journals Genetic Diversity of High and Low Molecular Weight Glutenin Subunits in Algerian Aegilops geniculata

2014 ◽  
Vol 42 (2) ◽  
pp. 453-459 ◽  
Author(s):  
Asma MEDOURI ◽  
Inès BELLIL ◽  
Douadi KHELIFI
Keyword(s):  
Molecular Weight ◽  
High Molecular Weight ◽  
Storage Proteins ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Low Molecular Weight ◽  
Glutenin Subunits ◽  
Bread Making ◽  
Aegilops Geniculata ◽  
Wide Range

Aegilops geniculata Roth is an annual grass relative to cultivated wheat and is widely distributed in North Algeria. Endosperm storage proteins of wheat and its relatives, namely glutenins and gliadins, play an important role in dough properties and bread making quality. In the present study, the different alleles encoded at the four glutenin loci (Glu-M1, Glu-U1, Glu-M3 and Glu-U3) were identified from thirty five accessions of the tetraploid wild wheat A. geniculata collected in Algeria using Sodium dodecyl Sulfate - Polyacrylamide Gel Electrophoresis (SDS-PAGE). At Glu-M1 and Glu-U1 loci, encoding high molecular weight glutenin subunits (HMW-GS) or A-subunits, 15 and 12 alleles were observed respectively, including one new subunit. B-Low molecular weight glutenin subunits zone (B-LMW-GS) displayed a far greater variation, as 28 and 25 alleles were identified at loci Glu-M3 and Glu-U3 respectively. Thirty two subunits patterns were revealed at the C subunits- zone and a total of thirty four patterns resulted from the genetic combination of the two zones (B- and C-zone). The wide range of glutenin subunits variation (high molecular weight glutenin subunits and low molecular weight glutenin subunits) in this species has the potential to enhance the genetic variability for improving the quality of wheat./span>

Mass Spectrometric Approaches for the Characterization of Low-Molecular Weight Glutenin Subunits

2011 ◽  
pp. 34-37 ◽  
Author(s):  
V. Cunsolo ◽  
S. Foti ◽  
V. Muccilli ◽  
R. Saletti ◽  
D. Lafiandra ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Mass Spectrometric ◽  
Low Molecular Weight ◽  
Glutenin Subunits
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