Characterization of a wheat mutant missing low-molecular-weight glutenin subunits encoded by the B-genome

2017 ◽  
Vol 73 ◽  
pp. 158-164 ◽  
Author(s):  
Jong-Yeol Lee ◽  
Chon-Sik Kang ◽  
Hye-Rang Beom ◽  
You-Ran Jang ◽  
Susan B. Altenbach ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Low Molecular Weight ◽  
Glutenin Subunits ◽  
B Genome ◽  
Wheat Mutant

Rapid Characterization of Wheat Low Molecular Weight Glutenin Subunits by Ultraperformance Liquid Chromatography (UPLC)

2013 ◽  
Vol 61 (17) ◽  
pp. 4026-4034 ◽  
Author(s):  
Zitong Yu ◽  
Caixia Han ◽  
Xing Yan ◽  
Xiaohui Li ◽  
Guoliang Jiang ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Liquid Chromatography ◽  
Low Molecular Weight ◽  
Glutenin Subunits ◽  
Rapid Characterization

scholarly journals Cloning and Characterization of Low-Molecular-Weight Glutenin Subunit Alleles from Chinese Wheat Landraces (Triticum aestivumL.)

2014 ◽  
Vol 2014 ◽  
pp. 1-6 ◽  
Author(s):  
Hongqi Si ◽  
Manli Zhao ◽  
Xin Zhang ◽  
Guoliang Yao ◽  
Genlou Sun ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Disulfide Bonds ◽  
Low Molecular Weight ◽  
Processing Quality ◽  
Repetitive Domain ◽  
Glutenin Subunits ◽  
Bread Making ◽  
Wheat Landraces ◽  
Chinese Wheat

Low-molecular-weight glutenin subunits (LMW-GS) are of great importance in processing quality and participate in the formation of polymers in wheat. In this study, eight new LMW-GS alleles were isolated from Chinese wheat landraces (Triticum aestivumL.) and designated asGlu-A3-1a,Glu-A3-1b,Glu-B3-1a,Glu-B3-1b,Glu-B3-1c,Glu-D3-1a,Glu-D3-1b, andGlu-D3-1c, which were located at theGlu-A3,Glu-B3, andGlu-D3loci, respectively. Based on the proteins encoded, the number of deduced amino acids ofGlu-B3alleles was approximately 50 more than those ofGlu-A3andGlu-D3alleles. The first cysteine ofGlu-A3andGlu-D3alleles was located at the N-terminal domain, while that ofGlu-B3alleles was found in the repetitive domain, which may lead to the different functioning in forming disulfide bonds. All the eight genes were LMW-m types and the new allele ofGlu-B3-1awhich had nine cysteine residues may be the desirable LMW-GS gene for improving bread-making quality.

Mass Spectrometric Approaches for the Characterization of Low-Molecular Weight Glutenin Subunits

2011 ◽  
pp. 34-37 ◽  
Author(s):  
V. Cunsolo ◽  
S. Foti ◽  
V. Muccilli ◽  
R. Saletti ◽  
D. Lafiandra ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Mass Spectrometric ◽  
Low Molecular Weight ◽  
Glutenin Subunits

Characterization of CIMMYT bread wheats for high- and low-molecular weight glutenin subunits and other quality-related genes with SDS-PAGE, RP-HPLC and molecular markers

Euphytica ◽  
2009 ◽  
Vol 172 (2) ◽  
pp. 235-250 ◽  
Author(s):  
Dan Liang ◽  
Jianwei Tang ◽  
Roberto Javier Peña ◽  
Ravi Singh ◽  
Xinyao He ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Molecular Markers ◽  
Low Molecular Weight ◽  
Glutenin Subunits ◽  
Rp Hplc ◽  
Sds Page

scholarly journals Characterization of high- and low-molecular-weight glutenin subunits from Chinese Xinjiang wheat landraces and historical varieties

2020 ◽  
Vol 57 (10) ◽  
pp. 3823-3835
Author(s):  
Shoufen Dai ◽  
Dongyang Xu ◽  
Yongliang Yan ◽  
Zhaojin Wen ◽  
Jinbo Zhang ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Low Molecular Weight ◽  
Glutenin Subunits ◽  
Wheat Landraces

scholarly journals Genetic diversity of high and low molecular weight glutenin subunits in Saharan bread and durum wheats from Algerian oases

2012 ◽  
Vol 48 (No. 1) ◽  
pp. 23-32 ◽  
Author(s):  
I. Bellil ◽  
M. Chekara Bouziani ◽  
D. Khelifi
Keyword(s):  
Genetic Diversity ◽  
Molecular Weight ◽  
Durum Wheat ◽  
Bread Wheat ◽  
Allelic Variation ◽  
Low Molecular Weight ◽  
Glutenin Subunits ◽  
Lmw Glutenin Subunits ◽  
Genotypic Identification ◽  
Diversity Studies

Saharan wheats have been studied particularly from a botanical viewpoint. Genotypic identification, classification and genetic diversity studies to date were essentially based on the morphology of the spike and grain. For this, the allelic variation at the glutenin loci was studied in a set of Saharan bread and durum wheats from Algerian oases where this crop has been traditionally cultivated. The high molecular weight and low molecular weight glutenin subunit composition of 40 Saharan bread and 30 durum wheats was determined by SDS-PAGE. In Saharan bread wheats 32 alleles at the six glutenin loci were detected, which in combination resulted in 36 different patterns including 17 for HMW and 23 for LMW glutenin subunits. For the Saharan durum wheats, 29 different alleles were identified for the five glutenin loci studied. Altogether, 29 glutenin patterns were detected, including 13 for HMW-GS and 20 for LMW-GS. Three new alleles were found in Saharan wheats, two in durum wheat at the Glu-B1 and Glu-B3 loci, and one in bread wheat at the Glu-B1 locus. The mean indices of genetic variation at the six loci in bread wheat and at the five loci in durum wheat were 0.59 and 0.63, respectively, showing that Saharan wheats were more diverse. This information could be useful to select Saharan varieties with improved quality and also as a source of genes to develop new lines when breeding for quality.

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