Characterization of low molecular weight polymers by liquid chromatography and field desorption mass spectroscopy

1979 ◽  
Vol 51 (8) ◽  
pp. 1293-1296 ◽  
Author(s):  
Robert P. Lattimer ◽  
Dale J. Harmon ◽  
Kenneth R. Welch
Keyword(s):  
Molecular Weight ◽  
Liquid Chromatography ◽  
Mass Spectroscopy ◽  
Low Molecular Weight ◽  
Field Desorption

scholarly journals Searching for Low Molecular Weight Seleno-Compounds in Sprouts by Mass Spectrometry

Molecules ◽  
2020 ◽  
Vol 25 (12) ◽  
pp. 2870 ◽  
Author(s):  
Eliza Kurek ◽  
Magdalena Michalska-Kacymirow ◽  
Anna Konopka ◽  
Olga Kościuczuk ◽  
Anna Tomiak ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Molecular Weight ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Analytical Techniques ◽  
Low Molecular Weight ◽  
Analytical Protocol ◽  
Icp Ms

A fit for purpose analytical protocol was designed towards searching for low molecular weight seleno-compounds in sprouts. Complementary analytical techniques were used to collect information enabling the characterization of selenium speciation. Conceiving the overall characterization of the behavior of selenium, inductively plasma optical mass spectrometry (ICP-MS) was used to determine the total selenium content in entire sprouts as well as in selected extracts or chromatographic fractions. Then, high-performance liquid chromatography combined with ICP-MS (HPLC-ICP-MS) was used to evaluate the presence of inorganic and organic seleno-compounds, with the advantages of being very sensitive towards selenium, but limited by available selenium standard compounds. Finally, ultra-high performance liquid chromatography electrospray ionization triple quadrupole mass spectrometry (UHPLC-ESI-QqQ-MS/MS) and UHPLC-ESI-Orbitrap-MS/MS were used for the confirmation of the identity of selected compounds and identification of several unknown compounds of selenium in vegetable sprouts (sunflower, onion, radish), respectively. Cultivation of plants was designed to supplement sprouts with selenium by using solutions of selenium (IV) at the concentration of 10, 20, 40, and 60 mg/L. The applied methodology allowed to justify that vegetable sprouts metabolize inorganic selenium to a number of organic derivatives, such as seleno-methylselenocysteine (SeMetSeCys), selenomethionine (SeMet), 5′-seleno-adenosine, 2,3-DHP-selenolanthionine, Se-S conjugate of cysteine-selenoglutathione, 2,3-DHP-selenocysteine-cysteine, 2,3-DHP-selenocysteine-cysteinealanine, glutathione-2,3-DHP-selenocysteine, gamma-Glu-MetSeCys or glutamyl-glycinyl-N-2,3-DHP-selenocysteine.

Rapid Characterization of Wheat Low Molecular Weight Glutenin Subunits by Ultraperformance Liquid Chromatography (UPLC)

2013 ◽  
Vol 61 (17) ◽  
pp. 4026-4034 ◽  
Author(s):  
Zitong Yu ◽  
Caixia Han ◽  
Xing Yan ◽  
Xiaohui Li ◽  
Guoliang Jiang ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Liquid Chromatography ◽  
Low Molecular Weight ◽  
Glutenin Subunits ◽  
Rapid Characterization
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