Rapid Determination of Hydrogen Peroxide Using Peroxidase Immobilized on Amberlite IRA-743 and Minerals in Honey

2007 ◽  
Vol 55 (17) ◽  
pp. 6885-6890 ◽  
Author(s):  
Rômulo Augusto de Abreu Franchini ◽  
Cabrini Ferraz de Souza ◽  
Rosana Colombara ◽  
Maria Auxiliadora Costa Matos ◽  
Renato Camargo Matos
Keyword(s):  
Hydrogen Peroxide ◽  
Rapid Determination

Rapid determination of the hypoxanthine increase in ischemic exercise tests.

1988 ◽  
Vol 34 (8) ◽  
pp. 1607-1610 ◽  
Author(s):  
P A Bolhuis ◽  
R Zwart ◽  
P R Bär ◽  
M de Visser ◽  
H J van der Helm
Keyword(s):  
Hydrogen Peroxide ◽  
Detection Limit ◽  
Rapid Determination ◽  
Serum Lactate ◽  
Benzenesulfonic Acid ◽  
Exercise Tests ◽  
Ph Optimum ◽  
Absorbance Maximum ◽  
Myoadenylate Deaminase

Abstract After ischemic exercise tests, performed to detect glycogenoses or myoadenylate deaminase (EC 3.5.4.6) deficiency, the increases in serum lactate and ammonia usually are measured. Determination of hypoxanthine instead of ammonia can also be used to show myoadenylate deaminase deficiency, but HPLC of hypoxanthine is time-consuming. As a substitute, we developed an indirect enzymatic equilibrium method for hypoxanthine based on coupling the chromogenic system 3,5-dichloro-2-hydroxy-benzenesulfonic acid/4-aminophenazone with formation of hydrogen peroxide by xanthine oxidase (EC 1.1.3.22). The pH optimum is at 7.8 and the absorbance maximum at 510 nm. The calibration curve is linear from 0 to 100 mumol/L and the detection limit is 0.9 mumol/L. Analytical variability (CV) was 1.5% to 3.6% within-run, 4.5% to 8.5% between-run. The assay can be performed with a standard spectrophotometer or a centrifugal analyzer. The coefficient of correlation was 0.68 between hypoxanthine and ammonia increases in plasma from controls who performed the exercise test.

A new route for simple and rapid determination of hydrogen peroxide in RAW264.7 macrophages by microchip electrophoresis

2009 ◽  
Vol 30 (11) ◽  
pp. 1983-1990 ◽  
Author(s):  
Xiaocong Gong ◽  
Qingling Li ◽  
Kehua Xu ◽  
Xin Liu ◽  
Hongmin Li ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Rapid Determination ◽  
Microchip Electrophoresis ◽  
Raw264.7 Macrophages

scholarly journals Simple and Rapid Determination of Hydrogen Peroxide Using Phosphine-based Fluorescent Reagents with Sodium Tungstate Dihydrate

2006 ◽  
Vol 22 (6) ◽  
pp. 815-817 ◽  
Author(s):  
Maki ONODA ◽  
Takefumi UCHIYAMA ◽  
Ken-ichi MAWATARI ◽  
Kiyoko KANEKO ◽  
Kazuya NAKAGOMI
Keyword(s):  
Hydrogen Peroxide ◽  
Sodium Tungstate ◽  
Rapid Determination

Rapid determination of trace amounts of hydrogen peroxide

1976 ◽  
Vol 85 (1) ◽  
pp. 179-183 ◽  
Author(s):  
K.G. Boto ◽  
L.F.G. Williams
Keyword(s):  
Hydrogen Peroxide ◽  
Rapid Determination

Synthesis of PB@FePt hybrid nanoparticles with peroxidase-mimicking activity for colorimetric determination of hydrogen peroxide in living cells

2019 ◽  
Vol 11 (5) ◽  
pp. 677-683 ◽  
Author(s):  
Zunfu Hu ◽  
Zhichao Dai ◽  
Xiaowei Hu ◽  
Kuiyong Chen ◽  
Chuanhui Gao ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Food Safety ◽  
Clinical Diagnosis ◽  
Rapid Determination ◽  
Living Cells ◽  
Hybrid Nanoparticles ◽  
Colorimetric Determination

The rapid determination of H2O2 is important in a series of areas, such as bioanalysis, food safety and clinical diagnosis.

Rapid Determination of Copper and Iron in Ore Leaching Solution by Iodometry

2013 ◽  
Vol 781-784 ◽  
pp. 2328-2331
Author(s):  
Cheng Ying Zhou ◽  
Wei Qu
Keyword(s):  
Hydrogen Peroxide ◽  
Good Accuracy ◽  
Rapid Determination ◽  
Accuracy And Precision ◽  
Relative Standard ◽  
Pretreatment Time ◽  
Leaching Solution ◽  
Pretreatment Temperature ◽  
Determination Of Copper

This paper provides a pretreatment method for ore leaching solution by adding an agent level of hydrogen peroxide. Pretreatment temperature, agent level of hydrogen peroxide and pretreatment time were investigated. The results show that relative standard deviation of copper or iron in ore leaching solution determined by iodometry is less than 1.00% with good accuracy and precision under the following conditions: pretreatment temperature of 60°C, agent level of hydrogen peroxide 1.0mL, pretreatment time of 30 minutes. This pretreatment process is simpler, faster, easier to control, and less environmental pollution comparing with the traditional pretreatment.

Sign in / Sign up

Export Citation Format

Share Document