A new route for simple and rapid determination of hydrogen peroxide in RAW264.7 macrophages by microchip electrophoresis

2009 ◽  
Vol 30 (11) ◽  
pp. 1983-1990 ◽  
Author(s):  
Xiaocong Gong ◽  
Qingling Li ◽  
Kehua Xu ◽  
Xin Liu ◽  
Hongmin Li ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Rapid Determination ◽  
Microchip Electrophoresis ◽  
Raw264.7 Macrophages

Rapid determination of the hypoxanthine increase in ischemic exercise tests.

1988 ◽  
Vol 34 (8) ◽  
pp. 1607-1610 ◽  
Author(s):  
P A Bolhuis ◽  
R Zwart ◽  
P R Bär ◽  
M de Visser ◽  
H J van der Helm
Keyword(s):  
Hydrogen Peroxide ◽  
Detection Limit ◽  
Rapid Determination ◽  
Serum Lactate ◽  
Benzenesulfonic Acid ◽  
Exercise Tests ◽  
Ph Optimum ◽  
Absorbance Maximum ◽  
Myoadenylate Deaminase

Abstract After ischemic exercise tests, performed to detect glycogenoses or myoadenylate deaminase (EC 3.5.4.6) deficiency, the increases in serum lactate and ammonia usually are measured. Determination of hypoxanthine instead of ammonia can also be used to show myoadenylate deaminase deficiency, but HPLC of hypoxanthine is time-consuming. As a substitute, we developed an indirect enzymatic equilibrium method for hypoxanthine based on coupling the chromogenic system 3,5-dichloro-2-hydroxy-benzenesulfonic acid/4-aminophenazone with formation of hydrogen peroxide by xanthine oxidase (EC 1.1.3.22). The pH optimum is at 7.8 and the absorbance maximum at 510 nm. The calibration curve is linear from 0 to 100 mumol/L and the detection limit is 0.9 mumol/L. Analytical variability (CV) was 1.5% to 3.6% within-run, 4.5% to 8.5% between-run. The assay can be performed with a standard spectrophotometer or a centrifugal analyzer. The coefficient of correlation was 0.68 between hypoxanthine and ammonia increases in plasma from controls who performed the exercise test.

scholarly journals Simple and Rapid Determination of Hydrogen Peroxide Using Phosphine-based Fluorescent Reagents with Sodium Tungstate Dihydrate

2006 ◽  
Vol 22 (6) ◽  
pp. 815-817 ◽  
Author(s):  
Maki ONODA ◽  
Takefumi UCHIYAMA ◽  
Ken-ichi MAWATARI ◽  
Kiyoko KANEKO ◽  
Kazuya NAKAGOMI
Keyword(s):  
Hydrogen Peroxide ◽  
Sodium Tungstate ◽  
Rapid Determination

Rapid Determination of Creatinine in Human Urine by Microchip Electrophoresis with LED Induced Fluorescence Detection

2012 ◽  
Vol 75 (21-22) ◽  
pp. 1287-1293 ◽  
Author(s):  
Shuping Wang ◽  
Xinchun Li ◽  
Jianping Yang ◽  
Xiujuan Yang ◽  
Fenghua Hou ◽  
...  
Keyword(s):  
Fluorescence Detection ◽  
Human Urine ◽  
Rapid Determination ◽  
Microchip Electrophoresis

Rapid determination of trace amounts of hydrogen peroxide

1976 ◽  
Vol 85 (1) ◽  
pp. 179-183 ◽  
Author(s):  
K.G. Boto ◽  
L.F.G. Williams
Keyword(s):  
Hydrogen Peroxide ◽  
Rapid Determination
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