Isolation and characterization of volatile sulfur-containing meat flavor components in model systems

1990 ◽  
Vol 38 (3) ◽  
pp. 777-791 ◽  
Author(s):  
Peter Werkhoff ◽  
Juergen Bruening ◽  
Roland Emberger ◽  
Matthias Guentert ◽  
Manfred Koepsel ◽  
...  
Keyword(s):  
Model Systems ◽  
Isolation And Characterization ◽  
Flavor Components ◽  
Meat Flavor ◽  
Sulfur Containing ◽  
Volatile Sulfur

scholarly journals Isolation and characterization of novel primary cells from the human distal outflow pathway

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Uttio Roy Chowdhury ◽  
Cindy K. Bahler ◽  
Cheryl R. Hann ◽  
Bradley H. Holman ◽  
Michael P. Fautsch
Keyword(s):  
Trabecular Meshwork ◽  
Distal Region ◽  
Proximal Region ◽  
Cellular Growth ◽  
Model Systems ◽  
Type I ◽  
Isolation And Characterization ◽  
Outflow Pathway

AbstractOcular hypertension occurs due to increased resistance to aqueous humor removal through the conventional outflow pathway. Unlike the proximal region of the conventional outflow pathway, the distal region has not been well studied, mostly due to lack of model systems. Here we describe isolation and characterization of human primary vascular distal outflow pathway (VDOP) cells from the distal region of the conventional outflow pathway. Tissue from the distal region was isolated from human corneo-scleral rims, digested with collagenase type I (100 U/ml) and placed on gelatin coated plates to allow cellular growth in Dulbecco’s Modified Eagle’s Medium (low glucose) containing fetal bovine serum and antibiotic/antimycotic. VDOP cells showed consistent proliferation for up to 7 passages, retained endothelial-like nature of the parent tissues and showed a unique marker phenotype of Lectin+VEGFR2-CD34-NG2- that was distinct from neighboring trabecular meshwork (Lectin+VEGFR2-CD34-NG2+) and Schlemm’s canal (Lectin+VEGFR2+CD34+NG2+) cells. Dexamethasone treated VDOP cells did not express myocilin and did not form cross-linked actin networks, in contrast to trabecular meshwork cells. These data show that VDOP cells are unique to the distal outflow region and can be used as a viable in vitro model system to understand the biology of the distal outflow pathway and intraocular pressure regulation.

scholarly journals Isolation and characterization of extracellular vesicles from Caenorhabditis elegans for multi-omic analysis

2018 ◽  
Author(s):  
Joshua C. Russell ◽  
Gennifer E. Merrihew ◽  
Julia E. Robbins ◽  
Nadia Postupna ◽  
Tyek-Kyun Kim ◽  
...  
Keyword(s):  
Cell Culture ◽  
Caenorhabditis Elegans ◽  
Extracellular Vesicles ◽  
Mammalian Cell Culture ◽  
Model Systems ◽  
C Elegans ◽  
Isolation And Characterization ◽  
Transmission Electron ◽  
Invertebrate Model

AbstractCells from bacteria to human release vesicles into their extracellular environment. These extracellular vesicles (EVs) contain multiple classesof molecules, including nucleic acids, proteins, and lipids. The isolation and analysis of EV cargos from mammalian cell culture and liquid biopsysamples has become a powerful approach for uncovering the messages that are packaged into these organelles. However, this approach has not been tenable in invertebrate model systems due to lack of sufficient amounts of pure EVs. Here we report a robust and reproducible procedure to isolateEVs from Caenorhabditis elegans with yields similar to those obtained from human cell culture. Through nanoparticle tracking, transmission electron microscopy, flow cytometry, mass spectrometry, RNAseq, and immunoaffinity analysis we provide the first ever detailed characterization of C. elegans EV composition and demonstrate that C. elegans EVs share fundamentally similar properties with their mammalian counterparts. These include vesicle size, enrichment for lipid rafts, and similar types of RNA and protein cargos. This ability of isolate pure EVs on ascale amenable to multiple types of downstream analyses permits, multi-omics characterization of EV cargos in an invertebrate model system.

scholarly journals Three Sulfur-Containing Natural Products From Rubus Hirsutus Thunb

2021 ◽  
Vol 16 (4) ◽  
pp. 1934578X2110113
Author(s):  
Na Lin ◽  
Sihui Li ◽  
Xuexia Chen ◽  
Jianfeng Song ◽  
Yichao Ge ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Density Functional ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Time Dependent ◽  
Functional Theory ◽  
Isolation And Characterization ◽  
Sulfur Containing ◽  
Mic Value ◽  
Dependent Density

Chemical investigation of the leaves and stems of Rubus hirsutus Thunb has resulted in the isolation and characterization of a rare new tricyclic thienocyclopentapyran (1) and 2 known thiophenes, 5-(3-buten-1-ynul)−2,2’-bithiophene (2) and α-terthienyl (3). The structure of compound 1 was unambiguously determined by nuclear magnetic resonance (NMR) spectroscopy, mass spectrometry and time-dependent density functional theory electronic circular dichroism calculations. The new compound 1 exhibited impressive antibiotic properties against methicillin resistant Staphylococcus aureus (MRSA), with an MIC value of around 1 µg/mL.

scholarly journals Cloning and Characterization of Two Lactobacillus casei Genes Encoding a Cystathionine Lyase

2007 ◽  
Vol 74 (1) ◽  
pp. 99-106 ◽  
Author(s):  
Stefan Irmler ◽  
Sylvie Raboud ◽  
Beata Beisert ◽  
Doris Rauhut ◽  
Hélène Berthoud
Keyword(s):  
Lactobacillus Casei ◽  
Sulfur Compounds ◽  
Ph Optimum ◽  
Genome Database ◽  
Volatile Sulfur Compounds ◽  
Genes Encoding ◽  
Cbl Proteins ◽  
Sulfur Containing ◽  
Volatile Sulfur

ABSTRACT Volatile sulfur compounds are key flavor compounds in several cheese types. To better understand the metabolism of sulfur-containing amino acids, which certainly plays a key role in the release of volatile sulfur compounds, we searched the genome database of Lactobacillus casei ATCC 334 for genes encoding putative homologs of enzymes known to degrade cysteine, cystathionine, and methionine. The search revealed that L. casei possesses two genes that putatively encode a cystathionine β-lyase (CBL; EC 4.4.1.8). The enzyme has been implicated in the degradation of not only cystathionine but also cysteine and methionine. Recombinant CBL proteins catalyzed the degradation of l-cystathionine, O-succinyl-l-homoserine, l-cysteine, l-serine, and l-methionine to form α-keto acid, hydrogen sulfide, or methanethiol. The two enzymes showed notable differences in substrate specificity and pH optimum.

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