scholarly journals Cloning and Characterization of Two Lactobacillus casei Genes Encoding a Cystathionine Lyase

2007 ◽  
Vol 74 (1) ◽  
pp. 99-106 ◽  
Author(s):  
Stefan Irmler ◽  
Sylvie Raboud ◽  
Beata Beisert ◽  
Doris Rauhut ◽  
Hélène Berthoud
Keyword(s):  
Lactobacillus Casei ◽  
Sulfur Compounds ◽  
Ph Optimum ◽  
Genome Database ◽  
Volatile Sulfur Compounds ◽  
Genes Encoding ◽  
Cbl Proteins ◽  
Sulfur Containing ◽  
Volatile Sulfur

ABSTRACT Volatile sulfur compounds are key flavor compounds in several cheese types. To better understand the metabolism of sulfur-containing amino acids, which certainly plays a key role in the release of volatile sulfur compounds, we searched the genome database of Lactobacillus casei ATCC 334 for genes encoding putative homologs of enzymes known to degrade cysteine, cystathionine, and methionine. The search revealed that L. casei possesses two genes that putatively encode a cystathionine β-lyase (CBL; EC 4.4.1.8). The enzyme has been implicated in the degradation of not only cystathionine but also cysteine and methionine. Recombinant CBL proteins catalyzed the degradation of l-cystathionine, O-succinyl-l-homoserine, l-cysteine, l-serine, and l-methionine to form α-keto acid, hydrogen sulfide, or methanethiol. The two enzymes showed notable differences in substrate specificity and pH optimum.

scholarly journals Halitosis in Children Undergoing Full Mouth Rehabilitation under General Anesthesia

Children ◽  
2021 ◽  
Vol 8 (2) ◽  
pp. 149
Author(s):  
Noura A. AlMadhi ◽  
Ayman M. Sulimany ◽  
Hamad A. Alzoman ◽  
Omar A. Bawazir
Keyword(s):  
General Anesthesia ◽  
Sulfur Compounds ◽  
Parental Consent ◽  
Inclusion Criteria ◽  
Breath Sample ◽  
Volatile Sulfur Compounds ◽  
Before And After ◽  
Full Mouth Rehabilitation ◽  
Volatile Sulfur

Interventions and management modalities of pediatric halitosis have been suggested in the literature, however, the effect of full mouth rehabilitation (FMR) under general anesthesia (GA) on pediatric halitosis was not reported. Therefore, the present study was conducted to investigate parents’ perceptions of their child’s halitosis before and after FMR under GA; and to evaluate the effect of FMR on clinical halitosis. Fifty-seven children between 3–8 years old, scheduled for FMR under GA, were included after satisfying the inclusion criteria and upon parental consent. Parents’ perception of halitosis in their children was evaluated using a standardized questionnaire and a breath sample was collected to assess the level of volatile sulfur compounds (VSCs) using OralChromaTM before and after FMR under GA. Sixty percent (n = 34) of the parents perceived halitosis in their children before FMR and about 80% (n = 27) of them reported improvement in halitosis after FMR. Clinical halitosis was detected in 84.2% (n = 48) of the sample before treatment. A statistically significant reduction in halitosis was found in 56.3% (n = 27) of the children after treatment (p < 0.001). In conclusion, majority of parents perceived an absence or reduction of halitosis in their children following FMR and significant improvement of clinical halitosis.

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