Tag-Reporter Strategy for Facile Oligosaccharide Synthesis on Polymer Support

2001 ◽  
Vol 123 (16) ◽  
pp. 3848-3849 ◽  
Author(s):  
Hiromune Ando ◽  
Shino Manabe ◽  
Yoshiaki Nakahara ◽  
Yukishige Ito
Keyword(s):  
Polymer Support ◽  
Oligosaccharide Synthesis

Efficient synthesis of the nonamannoside residue of high mannose glycoproteins

2005 ◽  
Vol 83 (6-7) ◽  
pp. 693-701 ◽  
Author(s):  
Lu Jiang ◽  
Tak Hang Chan
Keyword(s):  
Molecular Weight ◽  
Polyethylene Glycol ◽  
Key Words ◽  
Building Blocks ◽  
Polymer Support ◽  
Low Molecular Weight ◽  
Oligosaccharide Synthesis ◽  
Efficient Synthesis ◽  
Solution Synthesis ◽  
High Mannose

An efficient synthesis of the nonamannoside residue 1 of high mannose glycoproteins was achieved using an oligomer-supported solution synthesis with low-molecular-weight polyethylene glycol as the anchor. The synthesis required only five coupling steps from only two monosaccharide building blocks with an overall yield of 12%.Key words: nonamannoside, oligosaccharide synthesis, soluble polymer support synthesis, glycoproteins.

Oligosaccharide synthesis on a soluble, hyperbranched polymer support via thioglycoside activation

Tetrahedron ◽  
2005 ◽  
Vol 61 (35) ◽  
pp. 8329-8338 ◽  
Author(s):  
Eric Assen B. Kantchev ◽  
Scott J. Bader ◽  
Jon R. Parquette
Keyword(s):  
Hyperbranched Polymer ◽  
Polymer Support ◽  
Oligosaccharide Synthesis

scholarly journals The development of a dedicated polymer support for the solid-phase oligosaccharide synthesis

2020 ◽  
Vol 56 (72) ◽  
pp. 10568-10571 ◽  
Author(s):  
Matteo Panza ◽  
Dharmendra Neupane ◽  
Keith J. Stine ◽  
Alexei V. Demchenko
Keyword(s):  
Solid Phase ◽  
Polymer Support ◽  
Oligosaccharide Synthesis

Reported herein is the development of a novel polystyrene-based resin that we named PanzaGel.

scholarly journals β-Galactosidase-Producing Isolates in Mucoromycota: Screening, Enzyme Production, and Applications for Functional Oligosaccharide Synthesis

2021 ◽  
Vol 7 (3) ◽  
pp. 229
Author(s):  
Bettina Volford ◽  
Mónika Varga ◽  
András Szekeres ◽  
Alexandra Kotogán ◽  
Gábor Nagy ◽  
...  
Keyword(s):  
Wheat Bran ◽  
Enzyme Production ◽  
Specific Activity ◽  
Prebiotic Synthesis ◽  
Crude Enzyme ◽  
Oligosaccharide Synthesis ◽  
Industrial Enzymes ◽  
Chromogenic Agar ◽  
Lichtheimia Ramosa ◽  
Excellent Source

β-Galactosidases of Mucoromycota are rarely studied, although this group of filamentous fungi is an excellent source of many industrial enzymes. In this study, 99 isolates from the genera Lichtheimia, Mortierella, Mucor, Rhizomucor, Rhizopus and Umbelopsis, were screened for their β-galactosidase activity using a chromogenic agar approach. Ten isolates from the best producers were selected, and the activity was further investigated in submerged (SmF) and solid-state (SSF) fermentation systems containing lactose and/or wheat bran substrates as enzyme production inducers. Wheat bran proved to be efficient for the enzyme production under both SmF and SSF conditions, giving maximum specific activity yields from 32 to 12,064 U/mg protein and from 783 to 22,720 U/mg protein, respectively. Oligosaccharide synthesis tests revealed the suitability of crude β-galactosidases from Lichtheimia ramosa Szeged Microbiological Collection (SZMC) 11360 and Rhizomucor pusillus SZMC 11025 to catalyze transgalactosylation reactions. In addition, the crude enzyme extracts had transfructosylation activity, resulting in the formation of fructo-oligosaccharide molecules in a sucrose-containing environment. The maximal oligosaccharide concentration varied between 0.0158 and 2.236 g/L depending on the crude enzyme and the initial material. Some oligosaccharide-enriched mixtures supported the growth of probiotics, indicating the potential of the studied enzyme extracts in future prebiotic synthesis processes.

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