scholarly journals The development of a dedicated polymer support for the solid-phase oligosaccharide synthesis

2020 ◽  
Vol 56 (72) ◽  
pp. 10568-10571 ◽  
Author(s):  
Matteo Panza ◽  
Dharmendra Neupane ◽  
Keith J. Stine ◽  
Alexei V. Demchenko
Keyword(s):  
Solid Phase ◽  
Polymer Support ◽  
Oligosaccharide Synthesis

Reported herein is the development of a novel polystyrene-based resin that we named PanzaGel.

Use of controlled pore glass in solid phase oligosaccharide synthesis. Application to the semiautomated synthesis of a glyconucleotide conjugate

1998 ◽  
Vol 39 (14) ◽  
pp. 1953-1956 ◽  
Author(s):  
Matteo Adinolfi ◽  
Gaspare Barone ◽  
Lorenzo De Napoli ◽  
Alfonso Iadonisi ◽  
Gennaro Piccialli
Keyword(s):  
Solid Phase ◽  
Oligosaccharide Synthesis ◽  
Controlled Pore Glass ◽  
Pore Glass

scholarly journals An Improved Procedure for the Preparation of Thrombin Low Molecular Weight Substrates - Peptide p-Nitroanilides

2018 ◽  
Vol 1 (4) ◽  
pp. e00057 ◽  
Author(s):  
A.A Chistov ◽  
A.V. Talanova ◽  
M.V. Melnikova ◽  
S.S. Kuznetsova ◽  
E.F. Kolesanova
Keyword(s):  
Molecular Weight ◽  
Solid Phase ◽  
Solid Phase Peptide Synthesis ◽  
Potassium Sulfate ◽  
Polymer Support ◽  
Low Molecular Weight ◽  
Chromogenic Substrate ◽  
Peptide Substrates ◽  
Terminal Amino ◽  
Hydrolysis Of

Low molecular weight chromogenic thrombin peptide substrates, p-nitroanilides of short peptides protected at their N-terminal amino group, were prepared by solid-phase peptide synthesis on polystyrene-divinylbenzene polymer with trityl groups with preliminary attached p-phenylene diamine moiety. After the cleavage from the resin peptide p-aminoanilides were mildly oxidized to p-nitroanilides with the mixture of potassium sulfate and persulfate. Adsorption onto polymer support Bio-Beads SM-2 with further elution by acetonitrile allowed easy separating peptide p-nitroanilides from the oxidizer and obtaining the thrombin chromogenic substrate preparations with the target substance contents of not less than 95% and yields of 30-40%. Thrombin effectively catalyzed hydrolysis of the prepared substrates with KM and Vmax values of 29-134 mM and 0.03-1/16 mM/s, respectively.

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