Faculty Opinions recommendation of Oligosaccharide synthesis by coupled endo-glycosynthases of different specificity: a straightforward preparation of two mixed-linkage hexasaccharide substrates of 1,3/1,4-beta-glucanases.

2002 ◽  
Author(s):  
Stephen Withers
Keyword(s):  
Oligosaccharide Synthesis

scholarly journals β-Galactosidase-Producing Isolates in Mucoromycota: Screening, Enzyme Production, and Applications for Functional Oligosaccharide Synthesis

2021 ◽  
Vol 7 (3) ◽  
pp. 229
Author(s):  
Bettina Volford ◽  
Mónika Varga ◽  
András Szekeres ◽  
Alexandra Kotogán ◽  
Gábor Nagy ◽  
...  
Keyword(s):  
Wheat Bran ◽  
Enzyme Production ◽  
Specific Activity ◽  
Prebiotic Synthesis ◽  
Crude Enzyme ◽  
Oligosaccharide Synthesis ◽  
Industrial Enzymes ◽  
Chromogenic Agar ◽  
Lichtheimia Ramosa ◽  
Excellent Source

β-Galactosidases of Mucoromycota are rarely studied, although this group of filamentous fungi is an excellent source of many industrial enzymes. In this study, 99 isolates from the genera Lichtheimia, Mortierella, Mucor, Rhizomucor, Rhizopus and Umbelopsis, were screened for their β-galactosidase activity using a chromogenic agar approach. Ten isolates from the best producers were selected, and the activity was further investigated in submerged (SmF) and solid-state (SSF) fermentation systems containing lactose and/or wheat bran substrates as enzyme production inducers. Wheat bran proved to be efficient for the enzyme production under both SmF and SSF conditions, giving maximum specific activity yields from 32 to 12,064 U/mg protein and from 783 to 22,720 U/mg protein, respectively. Oligosaccharide synthesis tests revealed the suitability of crude β-galactosidases from Lichtheimia ramosa Szeged Microbiological Collection (SZMC) 11360 and Rhizomucor pusillus SZMC 11025 to catalyze transgalactosylation reactions. In addition, the crude enzyme extracts had transfructosylation activity, resulting in the formation of fructo-oligosaccharide molecules in a sucrose-containing environment. The maximal oligosaccharide concentration varied between 0.0158 and 2.236 g/L depending on the crude enzyme and the initial material. Some oligosaccharide-enriched mixtures supported the growth of probiotics, indicating the potential of the studied enzyme extracts in future prebiotic synthesis processes.

A New Oligosaccharide Synthesis Using Special Hydroxy Protecting Group.

ChemInform ◽  
2004 ◽  
Vol 35 (24) ◽  
Author(s):  
Shiro Komba ◽  
Motomitsu Kitaoka ◽  
Takafumi Kasumi
Keyword(s):  
Oligosaccharide Synthesis ◽  
Protecting Group

ChemInform Abstract: Use of 4-Nitrophenylthio-β-D-Glycosides in Oligosaccharide Synthesis: A Critical Evaluation.

ChemInform ◽  
2010 ◽  
Vol 25 (8) ◽  
pp. no-no
Author(s):  
L. A. J. M. SLIEDREGT ◽  
K. ZEGELAAR-JAARSVELD ◽  
G. A. VAN DER MAREL ◽  
H. H. VAN BOOM
Keyword(s):  
Critical Evaluation ◽  
Oligosaccharide Synthesis

Use of controlled pore glass in solid phase oligosaccharide synthesis. Application to the semiautomated synthesis of a glyconucleotide conjugate

1998 ◽  
Vol 39 (14) ◽  
pp. 1953-1956 ◽  
Author(s):  
Matteo Adinolfi ◽  
Gaspare Barone ◽  
Lorenzo De Napoli ◽  
Alfonso Iadonisi ◽  
Gennaro Piccialli
Keyword(s):  
Solid Phase ◽  
Oligosaccharide Synthesis ◽  
Controlled Pore Glass ◽  
Pore Glass

ChemInform Abstract: SUGAR ACETATES AS GLYCOSYLATING AGENTS IN OLIGOSACCHARIDE SYNTHESIS

1973 ◽  
Vol 4 (46) ◽  
pp. no-no
Author(s):  
V. A. NESMEYANOV ◽  
S. E. ZURABYAN ◽  
A. YA. KHORLIN
Keyword(s):  
Oligosaccharide Synthesis
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