Nitrous acid cross-links duplex DNA fragments through deoxyguanosine residues at the sequence 5'-CG

1991 ◽  
Vol 113 (12) ◽  
pp. 4681-4682 ◽  
Author(s):  
James J. Kirchner ◽  
Paul B. Hopkins
Keyword(s):  
Nitrous Acid ◽  
Duplex Dna ◽  
Dna Fragments ◽  
Cross Links

Mechlorethamine cross-links deoxyguanosine residues at 5'-GNC sequences in duplex DNA fragments

1990 ◽  
Vol 112 (6) ◽  
pp. 2459-2460 ◽  
Author(s):  
Julie T. Millard ◽  
Stanley Raucher ◽  
Paul B. Hopkins
Keyword(s):  
Duplex Dna ◽  
Dna Fragments ◽  
Cross Links

scholarly journals 4-vinyl-substituted pyrimidine nucleosides exhibit the efficient and selective formation of interstrand cross-links with RNA and duplex DNA

2013 ◽  
Vol 41 (13) ◽  
pp. 6774-6781 ◽  
Author(s):  
Atsushi Nishimoto ◽  
Daichi Jitsuzaki ◽  
Kazumitsu Onizuka ◽  
Yosuke Taniguchi ◽  
Fumi Nagatsugi ◽  
...  
Keyword(s):  
Duplex Dna ◽  
Pyrimidine Nucleosides ◽  
Interstrand Cross Links ◽  
Cross Links ◽  
Selective Formation

Chemical Synthesis and Preliminary Structural Characterization of a Nitrous Acid Interstrand Cross-Linked Duplex DNA

1999 ◽  
Vol 121 (21) ◽  
pp. 5081-5082 ◽  
Author(s):  
Eric A. Harwood ◽  
Snorri Th. Sigurdsson ◽  
N. B. Fredrik Edfeldt ◽  
Brian R. Reid ◽  
Paul B. Hopkins
Keyword(s):  
Chemical Synthesis ◽  
Structural Characterization ◽  
Nitrous Acid ◽  
Duplex Dna

scholarly journals Chemical and structural characterization of interstrand cross-links formed between abasic sites and adenine residues in duplex DNA

2015 ◽  
Vol 43 (7) ◽  
pp. 3434-3441 ◽  
Author(s):  
Nathan E. Price ◽  
Michael J. Catalano ◽  
Shuo Liu ◽  
Yinsheng Wang ◽  
Kent S. Gates
Keyword(s):  
Structural Characterization ◽  
Duplex Dna ◽  
Abasic Sites ◽  
Interstrand Cross Links ◽  
Cross Links

Characterization of DNA−Protein Cross-Links Induced by Oxanine:  Cellular Damage Derived from Nitric Oxide and Nitrous Acid†

Biochemistry ◽  
2007 ◽  
Vol 46 (13) ◽  
pp. 3952-3965 ◽  
Author(s):  
Hauh-Jyun Candy Chen ◽  
Chia-Jong Hsieh ◽  
Li-Ching Shen ◽  
Chia-Ming Chang
Keyword(s):  
Nitric Oxide ◽  
Nitrous Acid ◽  
Cellular Damage ◽  
Cross Links

scholarly journals Interaction of RecBCD Enzyme with DNA at Double-Strand Breaks Produced in UV-Irradiated Escherichia coli: Requirement for DNA End Processing

1998 ◽  
Vol 180 (21) ◽  
pp. 5639-5645 ◽  
Author(s):  
Brigitte Thoms ◽  
Wilfried Wackernagel
Keyword(s):  
Excision Repair ◽  
Uv Light ◽  
Single Strand ◽  
Duplex Dna ◽  
Dna Fragments ◽  
Chromosomal Dna ◽  
Phage T4 ◽  
Recbcd Enzyme ◽  
Dna Ends ◽  
Activity Decrease

ABSTRACT The RecBCD enzyme has a powerful duplex DNA exonuclease activity in vivo. We found that this activity decreased strongly when cells were irradiated with UV light (135 J/m2). The activity decrease was seen by an increase in survival of phage T42 − of about 200-fold (phage T42 − has defective duplex DNA end-protecting gene 2 protein). The activity decrease depended on excision repair proficiency of the cells and a postirradiation incubation. During this time, chromosome fragmentation occurred as demonstrated by pulsed-field gel electrophoresis. In accord with previous observations, it was concluded that the RecBCD enzyme is silenced during interaction with duplex DNA fragments containing Chi nucleotide sequences. The silencing was suppressed by induction or permanent derepression of the SOS system or by the overproduction of single-strand DNA binding protein (from a plasmid with ssb +) which is known to inhibit degradation of chromosomal DNA by cellular DNases. Further, mutations in xonA, recJ, andsbcCD, particularly in the recJ sbcCD andxonA recJ sbcCD combinations, impeded RecBCD silencing. The findings suggest that the DNA fragments had single-stranded tails of a length which prevents loading of RecBCD. It is concluded that in wild-type cells the tails are effectively removed by single-strand-specific DNases including exonuclease I, RecJ DNase, and SbcCD DNase. By this, tailed DNA ends are processed to entry sites for RecBCD. It is proposed that end blunting functions to direct DNA ends into the RecABCD pathway. This pathway specifically activates Chi-containing regions for recombination and recombinational repair.

scholarly journals Cho Endonuclease Functions during DNA Interstrand Cross-Link Repair in Escherichia coli

2016 ◽  
Vol 198 (22) ◽  
pp. 3099-3108 ◽  
Author(s):  
Anthonige Vidya Perera ◽  
James Brian Mendenhall ◽  
Charmain Tan Courcelle ◽  
Justin Courcelle
Keyword(s):  
Nucleotide Excision Repair ◽  
Excision Repair ◽  
Repair Process ◽  
Differential Sensitivity ◽  
Duplex Dna ◽  
Dna Lesion ◽  
Nucleotide Excision ◽  
Interstrand Cross Links ◽  
Cross Links ◽  
Complex Lesions

ABSTRACTDNA interstrand cross-links are complex lesions that covalently link both strands of the duplex DNA. Lesion removal is proposed to be initiated via the UvrABC nucleotide excision repair complex; however, less is known about the subsequent steps of this complex repair pathway. In this study, we characterized the contribution of nucleotide excision repair mutants to survival in the presence of psoralen-induced damage. Unexpectedly, we observed that the nucleotide excision repair mutants exhibit differential sensitivity to psoralen-induced damage, withuvrCmutants being less sensitive than eitheruvrAoruvrB. We show that Cho, an alternative endonuclease, acts with UvrAB and is responsible for the reduced hypersensitivity ofuvrCmutants. We find that Cho's contribution to survival correlates with the presence of DNA interstrand cross-links, rather than monoadducts, and operates at a step after, or independently from, the initial incision during the global repair of psoralen DNA adducts from the genome.IMPORTANCEDNA interstrand cross-links are complex lesions that covalently bind to both strands of the duplex DNA and whose mechanism of repair remains poorly understood. In this study, we show that Cho, an alternative endonuclease, acts with UvrAB and participates in the repair of DNA interstrand cross-links formed in the presence of photoactivated psoralens. Cho's contribution to survival correlates with the presence of DNA interstrand cross-links and operates at a step after, or independently from, the initial incision during the repair process.

scholarly journals Interstrand Cross-Links Generated by Abasic Sites in Duplex DNA

2007 ◽  
Vol 129 (7) ◽  
pp. 1852-1853 ◽  
Author(s):  
Sanjay Dutta ◽  
Goutam Chowdhury ◽  
Kent S. Gates
Keyword(s):  
Duplex Dna ◽  
Abasic Sites ◽  
Interstrand Cross Links ◽  
Cross Links
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