scholarly journals Interstrand Cross-Links Generated by Abasic Sites in Duplex DNA

2007 ◽  
Vol 129 (7) ◽  
pp. 1852-1853 ◽  
Author(s):  
Sanjay Dutta ◽  
Goutam Chowdhury ◽  
Kent S. Gates
Keyword(s):  
Duplex Dna ◽  
Abasic Sites ◽  
Interstrand Cross Links ◽  
Cross Links

scholarly journals Chemical and structural characterization of interstrand cross-links formed between abasic sites and adenine residues in duplex DNA

2015 ◽  
Vol 43 (7) ◽  
pp. 3434-3441 ◽  
Author(s):  
Nathan E. Price ◽  
Michael J. Catalano ◽  
Shuo Liu ◽  
Yinsheng Wang ◽  
Kent S. Gates
Keyword(s):  
Structural Characterization ◽  
Duplex Dna ◽  
Abasic Sites ◽  
Interstrand Cross Links ◽  
Cross Links

scholarly journals Interstrand cross-links arising from strand breaks at true abasic sites in duplex DNA

2017 ◽  
Vol 45 (11) ◽  
pp. 6275-6283 ◽  
Author(s):  
Zhiyu Yang ◽  
Nathan E. Price ◽  
Kevin M. Johnson ◽  
Yinsheng Wang ◽  
Kent S. Gates
Keyword(s):  
Duplex Dna ◽  
Strand Breaks ◽  
Abasic Sites ◽  
Interstrand Cross Links ◽  
Cross Links

scholarly journals Chemical Structure and Properties of Interstrand Cross-Links Formed by Reaction of Guanine Residues with Abasic Sites in Duplex DNA

2015 ◽  
Vol 137 (11) ◽  
pp. 3933-3945 ◽  
Author(s):  
Michael J. Catalano ◽  
Shuo Liu ◽  
Nisana Andersen ◽  
Zhiyu Yang ◽  
Kevin M. Johnson ◽  
...  
Keyword(s):  
Chemical Structure ◽  
Duplex Dna ◽  
Structure And Properties ◽  
Abasic Sites ◽  
Interstrand Cross Links ◽  
Cross Links

scholarly journals 4-vinyl-substituted pyrimidine nucleosides exhibit the efficient and selective formation of interstrand cross-links with RNA and duplex DNA

2013 ◽  
Vol 41 (13) ◽  
pp. 6774-6781 ◽  
Author(s):  
Atsushi Nishimoto ◽  
Daichi Jitsuzaki ◽  
Kazumitsu Onizuka ◽  
Yosuke Taniguchi ◽  
Fumi Nagatsugi ◽  
...  
Keyword(s):  
Duplex Dna ◽  
Pyrimidine Nucleosides ◽  
Interstrand Cross Links ◽  
Cross Links ◽  
Selective Formation

scholarly journals Cho Endonuclease Functions during DNA Interstrand Cross-Link Repair in Escherichia coli

2016 ◽  
Vol 198 (22) ◽  
pp. 3099-3108 ◽  
Author(s):  
Anthonige Vidya Perera ◽  
James Brian Mendenhall ◽  
Charmain Tan Courcelle ◽  
Justin Courcelle
Keyword(s):  
Nucleotide Excision Repair ◽  
Excision Repair ◽  
Repair Process ◽  
Differential Sensitivity ◽  
Duplex Dna ◽  
Dna Lesion ◽  
Nucleotide Excision ◽  
Interstrand Cross Links ◽  
Cross Links ◽  
Complex Lesions

ABSTRACTDNA interstrand cross-links are complex lesions that covalently link both strands of the duplex DNA. Lesion removal is proposed to be initiated via the UvrABC nucleotide excision repair complex; however, less is known about the subsequent steps of this complex repair pathway. In this study, we characterized the contribution of nucleotide excision repair mutants to survival in the presence of psoralen-induced damage. Unexpectedly, we observed that the nucleotide excision repair mutants exhibit differential sensitivity to psoralen-induced damage, withuvrCmutants being less sensitive than eitheruvrAoruvrB. We show that Cho, an alternative endonuclease, acts with UvrAB and is responsible for the reduced hypersensitivity ofuvrCmutants. We find that Cho's contribution to survival correlates with the presence of DNA interstrand cross-links, rather than monoadducts, and operates at a step after, or independently from, the initial incision during the global repair of psoralen DNA adducts from the genome.IMPORTANCEDNA interstrand cross-links are complex lesions that covalently bind to both strands of the duplex DNA and whose mechanism of repair remains poorly understood. In this study, we show that Cho, an alternative endonuclease, acts with UvrAB and participates in the repair of DNA interstrand cross-links formed in the presence of photoactivated psoralens. Cho's contribution to survival correlates with the presence of DNA interstrand cross-links and operates at a step after, or independently from, the initial incision during the repair process.

scholarly journals Chemical and strutural properties of DNA-abasic site cross-links

2014 ◽  
Author(s):  
◽  
Nathan Price
Keyword(s):  
Gel Electrophoresis ◽  
Structural Information ◽  
Sequence Specificity ◽  
Abasic Site ◽  
Cross Link ◽  
Dna Sequence Specificity ◽  
Abasic Sites ◽  
Interstrand Cross Links ◽  
Cross Links ◽  
Synthetic Standard

Here, we report on the formation of DNA interstrand cross-links. These cross-links form from abasic sites, an endogenous type of DNA damage. We have utilized gel electrophoresis, NMR, mass spectrometry and liquid chromatography to identify the location, DNA sequence specificity, stability and some structural information for these interstrand cross-links. Additionally, we have synthesized a small molecule standard which is identical to the enzymatically digested cross-link from duplex DNA. This synthetic standard can be used to increase sensitivity for future detection of DNA-abasic site cross-links.

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