Characterization of DNA−Protein Cross-Links Induced by Oxanine:  Cellular Damage Derived from Nitric Oxide and Nitrous Acid†

Biochemistry ◽  
2007 ◽  
Vol 46 (13) ◽  
pp. 3952-3965 ◽  
Author(s):  
Hauh-Jyun Candy Chen ◽  
Chia-Jong Hsieh ◽  
Li-Ching Shen ◽  
Chia-Ming Chang
Keyword(s):  
Nitric Oxide ◽  
Nitrous Acid ◽  
Cellular Damage ◽  
Cross Links

Isolation and Characterization of Diazoate Intermediate upon Nitrous Acid and Nitric Oxide Treatment of 2‘-Deoxycytidine†

Biochemistry ◽  
1999 ◽  
Vol 38 (22) ◽  
pp. 7151-7158 ◽  
Author(s):  
Toshinori Suzuki ◽  
Takanori Nakamura ◽  
Masaki Yamada ◽  
Hiroshi Ide ◽  
Kenji Kanaori ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Nitrous Acid ◽  
Isolation And Characterization

PHYSIOLOGIC AND MOLECULAR CHARACTERIZATION OF THE ROLE OF NITRIC OXIDE IN HEMORRHAGIC SHOCK

Shock ◽  
1997 ◽  
Vol 7 (3) ◽  
pp. 157-163 ◽  
Author(s):  
Edward Kelly ◽  
Nishit S. Shah ◽  
Nathan N. Morgan ◽  
Simon C. Watkins ◽  
Andrew B. Peitzman ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Hemorrhagic Shock ◽  
Molecular Characterization

scholarly journals A novel long intergenic non-coding RNA, Nostrill, regulates iNOS gene transcription and neurotoxicity in microglia

2021 ◽  
Vol 18 (1) ◽  
Author(s):  
Nicholas W. Mathy ◽  
Olivia Burleigh ◽  
Andrew Kochvar ◽  
Erin R. Whiteford ◽  
Matthew Behrens ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
No Production ◽  
Cortical Tissue ◽  
Non Coding Rna ◽  
Transcriptional Regulatory ◽  
Lncrna Locus ◽  
Rna Immunoprecipitation ◽  
Long Non Coding Rna

Abstract Background Microglia are resident immunocompetent and phagocytic cells in the CNS. Pro-inflammatory microglia, stimulated by microbial signals such as bacterial lipopolysaccharide (LPS), viral RNAs, or inflammatory cytokines, are neurotoxic and associated with pathogenesis of several neurodegenerative diseases. Long non-coding RNAs (lncRNA) are emerging as important tissue-specific regulatory molecules directing cell differentiation and functional states and may help direct proinflammatory responses of microglia. Characterization of lncRNAs upregulated in proinflammatory microglia, such as NR_126553 or 2500002B13Rik, now termed Nostrill (iNOS Transcriptional Regulatory Intergenic LncRNA Locus) increases our understanding of molecular mechanisms in CNS innate immunity. Methods Microglial gene expression array analyses and qRT-PCR were used to identify a novel long intergenic non-coding RNA, Nostrill, upregulated in LPS-stimulated microglial cell lines, LPS-stimulated primary microglia, and LPS-injected mouse cortical tissue. Silencing and overexpression studies, RNA immunoprecipitation, chromatin immunoprecipitation, chromatin isolation by RNA purification assays, and qRT-PCR were used to study the function of this long non-coding RNA in microglia. In vitro assays were used to examine the effects of silencing the novel long non-coding RNA in LPS-stimulated microglia on neurotoxicity. Results We report here characterization of intergenic lncRNA, NR_126553, or 2500002B13Rik now termed Nostrill (iNOS Transcriptional Regulatory Intergenic LncRNA Locus). Nostrill is induced by LPS stimulation in BV2 cells, primary murine microglia, and in cortical tissue of LPS-injected mice. Induction of Nostrill is NF-κB dependent and silencing of Nostrill decreased inducible nitric oxide synthase (iNOS) expression and nitric oxide (NO) production in BV2 and primary microglial cells. Overexpression of Nostrill increased iNOS expression and NO production. RNA immunoprecipitation assays demonstrated that Nostrill is physically associated with NF-κB subunit p65 following LPS stimulation. Silencing of Nostrill significantly reduced NF-κB p65 and RNA polymerase II recruitment to the iNOS promoter and decreased H3K4me3 activating histone modifications at iNOS gene loci. In vitro studies demonstrated that silencing of Nostrill in microglia reduced LPS-stimulated microglial neurotoxicity. Conclusions Our data indicate a new regulatory role of the NF-κB-induced Nostrill and suggest that Nostrill acts as a co-activator of transcription of iNOS resulting in the production of nitric oxide by microglia through modulation of epigenetic chromatin remodeling. Nostrill may be a target for reducing the neurotoxicity associated with iNOS-mediated inflammatory processes in microglia during neurodegeneration.

Characterization of Nitric Oxide (NO)-Induced Cell Death in Lung Epithelial Cells

2004 ◽  
Vol 56 (2) ◽  
pp. 187
Author(s):  
Wha Shim Yong ◽  
Youn Seup Kim ◽  
Jae Seuk Park ◽  
Young Koo Jee ◽  
Kye Young Lee
Keyword(s):  
Nitric Oxide ◽  
Cell Death ◽  
Epithelial Cells ◽  
Lung Epithelial Cells ◽  
Lung Epithelial

Characterization of the influence of nitric oxide donors on intestinal absorption of macromolecules

2004 ◽  
Vol 286 (1-2) ◽  
pp. 89-97 ◽  
Author(s):  
Koichi Takahashi ◽  
Nanako Numata ◽  
Natsumi Kinoshita ◽  
Naoki Utoguchi ◽  
Tadanori Mayumi ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Intestinal Absorption ◽  
Nitric Oxide Donors
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