SEMICONDUCTOR NANOPARTICLES AS REPORTERS IN MULTIPLEXED IMMUNOASSAY AND CELL ANALYSIS

2009 ◽  
Vol 08 (01n02) ◽  
pp. 163-167 ◽  
Author(s):  
TIM DUBROVSKY
Keyword(s):  
Quantum Dots ◽  
Research And Development ◽  
Spectral Properties ◽  
Solid Phase ◽  
Photophysical Properties ◽  
Cell Analysis ◽  
Biological Applications ◽  
Solid Phase Immunoassay ◽  
Multiplexed Immunoassay ◽  
Stokes Shifts

The development of new fluorophores has experienced a tremendous advance over the last two decades. Here, we discuss the photophysical properties of traditional fluorescent labels and the comparatively advantageous properties of quantum dots. The unique spectral properties of quantum dots, such as their large Stokes shifts and exceptional brightness, make them attractive probes in biological applications and prime candidates for further research and development in the field of solid-phase immunoassay and cell analysis.

Near-Infrared Heptamethine Cyanine Dyes: A New Tracer for Solid-Phase Immunoassays

1997 ◽  
Vol 51 (6) ◽  
pp. 836-843 ◽  
Author(s):  
Richard J. Williams ◽  
Jose Mauro Peralta ◽  
Victor C. W. Tsang ◽  
Narasimhachari Narayanan ◽  
Guillermo A. Casay ◽  
...  
Keyword(s):  
Spectral Properties ◽  
Near Infrared ◽  
Solid Phase ◽  
Human Immunoglobulin ◽  
Minimal Amount ◽  
Human Antibody ◽  
Near Ir ◽  
Solid Phase Immunoassay ◽  
Bioanalytical Applications ◽  
Aqueous Conditions

Near-infrared (near-IR) fluorescence has been used to develop a solid-phase immunoassay that detects trace amounts of human immunoglobulin (HuIgG). Various concentrations of HuIgG bound to a nitrocellulose surface were determined from the fluorescence generated by near-IR labeled goat anti-human antibody (GAHG) bound to the HuIgG. The GAHG was labeled with a heptamethine cyanine fluorophore that has spectral properties in the near-IR region (above 780 nm). These fluorophores are versatile because they can be modified for several bioanalytical applications. Fluorescence was detected with a near-IR fluorescence instrument previously developed in the laboratory. Two cyanine fluorophore labels were evaluated for the ability to selectively bind to GAHG on a nitrocellulose matrix with a minimal amount of background interference. After the most appropriate near-IR fluorophore was selected, the labeling of GAHG was optimized under aqueous conditions. The most effective GAHG–dye conjugates were used to develop an immunoassay to detect various concentrations of HuIgG. The results are presented, here. Solutions of HuIgG with concentrations as low as 10−10 molar have been detected with a minimum of interference.

Preparation of Graphene Quantum Dots and Their Biological Applications

2016 ◽  
Vol 31 (4) ◽  
pp. 337 ◽  
Author(s):  
SUN Xiao-Dan ◽  
LIU Zhong-Qun ◽  
YAN Hao
Keyword(s):  
Quantum Dots ◽  
Graphene Quantum Dots ◽  
Biological Applications

Electrodeposition immobilized molybdenum disulfide quantum dots and their electrochemiluminescence application in the detection of melamine residues in milk powder

2021 ◽  
Author(s):  
Yousheng Ye ◽  
Shasha Li ◽  
An Ping ◽  
Xinjun Wan ◽  
Jianguo Li
Keyword(s):  
Quantum Dots ◽  
Quantum Dot ◽  
Molybdenum Disulfide ◽  
Solid Phase ◽  
Milk Powder ◽  
One Step

In this paper, one-step hydrothermal and electrodeposition methods were used to prepare a MoS2 quantum dot (QD) solid-phase electrochemiluminescent (ECL) electrode for the detection of melamine residues in milk powder.

Performance Evaluation of the Phosphorescent Porphyrin Label:  Solid-Phase Immunoassay of α-Fetoprotein

2002 ◽  
Vol 74 (22) ◽  
pp. 5845-5850 ◽  
Author(s):  
Tomás C. O'Riordan ◽  
Aleksi E. Soini ◽  
Dmitri B. Papkovsky
Keyword(s):  
Performance Evaluation ◽  
Solid Phase ◽  
Solid Phase Immunoassay

Aqueous synthesis of highly luminescent AgInS2–ZnS quantum dots and their biological applications

Nanoscale ◽  
2013 ◽  
Vol 5 (6) ◽  
pp. 2322 ◽  
Author(s):  
Michelle D. Regulacio ◽  
Khin Yin Win ◽  
Seong Loong Lo ◽  
Shuang-Yuan Zhang ◽  
Xinhai Zhang ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Biological Applications ◽  
Aqueous Synthesis

A solid-phase immunoassay of protease-resistant prion protein with filtration blotting involving sodium dodecyl sulfate

2006 ◽  
Vol 349 (2) ◽  
pp. 218-228 ◽  
Author(s):  
Yoshiteru Kobayashi ◽  
Naoyuki Kohno ◽  
Shoko Wanibe ◽  
Kazunari Hirayasu ◽  
Hitoshi Uemori ◽  
...  
Keyword(s):  
Sodium Dodecyl Sulfate ◽  
Prion Protein ◽  
Solid Phase ◽  
Sodium Dodecyl ◽  
Solid Phase Immunoassay ◽  
Dodecyl Sulfate

Safe Synthesis of InP Quantum Dots for Biological Applications

2021 ◽  
Vol MA2021-01 (23) ◽  
pp. 915-915
Author(s):  
Hashini Bhagya Chandrasiri ◽  
Eun Byoel Kim ◽  
Preston Snee
Keyword(s):  
Quantum Dots ◽  
Biological Applications ◽  
Inp Quantum Dots

scholarly journals The nanoscaled metal-organic framework ICR-2 as a carrier of porphyrins for photodynamic therapy

2018 ◽  
Vol 9 ◽  
pp. 2960-2967 ◽  
Author(s):  
Jan Hynek ◽  
Sebastian Jurík ◽  
Martina Koncošová ◽  
Jaroslav Zelenka ◽  
Ivana Křížová ◽  
...  
Keyword(s):  
Inorganic Chemistry ◽  
Photodynamic Therapy ◽  
Photophysical Properties ◽  
Metal Organic Framework ◽  
Metal Organic Frameworks ◽  
Biological Applications ◽  
Nanoparticle Surface ◽  
Metal Organic ◽  
Unsaturated Metal Sites ◽  
First Time

Nanosized porphyrin-containing metal-organic frameworks (MOFs) attract considerable attention as solid-state photosensitizers for biological applications. In this study, we have for the first time synthesised and characterised phosphinate-based MOF nanoparticles, nanoICR-2 (Inorganic Chemistry Rez). We demonstrate that nanoICR-2 can be decorated with anionic 5,10,15,20-tetrakis(4-R-phosphinatophenyl)porphyrins (R = methyl, isopropyl, phenyl) by utilizing unsaturated metal sites on the nanoparticle surface. The use of these porphyrins allows for superior loading of the nanoparticles when compared with commonly used 5,10,15,20-tetrakis(4-carboxyphenyl)porphyrin. The nanoICR-2/porphyrin composites retain part of the free porphyrins photophysical properties, while the photodynamic efficacy is strongly affected by the R substituent at the porphyrin phosphinate groups. Thus, phosphinatophenylporphyrin with phenyl substituents has the strongest photodynamic efficacy due to the most efficient cellular uptake.

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