Interaction of kanchanomycin with nucleic acids. III. Contrasts in the mechanisms of inhibition of ribonucleic acid and deoxyribonucleic acid polymerase reactions

Biochemistry ◽  
1970 ◽  
Vol 9 (22) ◽  
pp. 4421-4427 ◽  
Author(s):  
Irving H. Goldberg ◽  
Peteranne B. Joel ◽  
Paul A. Friedman
Keyword(s):  
Nucleic Acids ◽  
Ribonucleic Acid ◽  
Deoxyribonucleic Acid

Changes in the nucleic acid content of the uterus of sheep during involution following ovariectomy and following oestrogenic stimulation

1976 ◽  
Vol 27 (5) ◽  
pp. 669 ◽  
Author(s):  
DA Little ◽  
LJ Lambourne
Keyword(s):  
Nucleic Acid ◽  
Nucleic Acids ◽  
Ribonucleic Acid ◽  
Acid Content ◽  
Deoxyribonucleic Acid ◽  
Total Content ◽  
Mean Value ◽  
Nucleic Acid Content ◽  
Uterine Tissue ◽  
Rna Concentration

The concentrations of nucleic acids, deoxyribonucleic acid (DNA) and ribonucleic acid (RNA), in ovine uterine tissue were studied to determine whether the ratio RNA/DNA might be used in the bioassay of oestrogenic activity. As the uterus decreased in size with time after ovariectomy, the concentration of DNA increased markedly, but the total content of DNA in the uterus remained constant (mean value 342 ? 8 (SE) mg). The RNA concentration of the tissue decreased during involution, with the result that a highly significant reduction of 33% in the RNA/DNA ratio occurred within 2 weeks of ovariectomy. The ratio decreased further with time. Other ewes were treated for 3 days with diethylstilboestrol dipropionate (10 µg/day), commencing 2 weeks after ovariectomy; the stage of the oestrous cycle at which the ovaries were removed had no effect on the response to the oestrogen. Changes in the nucleic acid concentrations in the uteri of these ewes, and the results obtained during involution, indicate that the RNA/DNA ratio of uterine tissue in ovariectomized ewes has potential as a basis for the bioassay of materials of low oestrogenic potency.

scholarly journals Effect of denervation on the synthesis of ribonucleic acid and deoxyribonucleic acid in rat diaphragm muscle

1968 ◽  
Vol 108 (2) ◽  
pp. 177-183 ◽  
Author(s):  
K L Manchester ◽  
E J Harris
Keyword(s):  
Nucleic Acids ◽  
Ribonucleic Acid ◽  
Orotic Acid ◽  
Deoxyribonucleic Acid ◽  
Diaphragm Muscle ◽  
Unit Mass ◽  
Rat Diaphragm ◽  
The Third

1. The effect of unilateral denervation of rat diaphragm muscle on its content of nucleic acids and their incorporation of precursors was investigated. 2. After denervation the paralysed hemidiaphragm hypertrophies and within 3 days its content of RNA increases considerably. The concentration of DNA/unit mass remains fairly constant. 3. During this period there is some increase in the rate of incorporation of [14C]adenine into RNA, whereas there is some diminution in the rate of incorporation of [14C]orotic acid. 4. Incorporation of [14C]adenine and [3H]thymidine into DNA is much increased in the paralysed tissue, reaching its maximum by about the third day, but returning to normal by the tenth. 5. The significance of these results in relation to the hypertrophy after denervation is discussed.

Nucleic Acids in Grass and Grass Silage

1988 ◽  
Vol 1988 ◽  
pp. 86-86
Author(s):  
A B McAllan ◽  
G D Braithwaite
Keyword(s):  
Nucleic Acid ◽  
Nucleic Acids ◽  
Protein Content ◽  
Ribonucleic Acid ◽  
Protein Fraction ◽  
Dry Matter ◽  
Deoxyribonucleic Acid ◽  
Total N ◽  
Grass Silage ◽  
Microbial N

Little attention has been directed at defining the ‘protein’ fraction of silages. This component is normally estimated by fractionation based on solubility characteristics and under the conditions most commonly used, nucleic acids (ribonucleic acid (RNA) and deoxyribonucleic acid (DNA)) would appear in the protein fraction. Grasses and legumes can contain appreciable amounts of nucleic acids ranging from 11-29 and 19-53 g/kg dry matter respectively (McAllan, 1982). No information is available as to the effects of ensilage on these nucleic acids. Microbes also contain appreciable amounts of nucleic acids which can account for 150-200 gN/kg total-N of the cell and these amounts may vary according to the stage of growth. It has been suggested (Ullrich, 1982) that microbial-N contribution to the total-N content of silage is as much as 220-280 g/kg. Thus the total amount of silage ‘protein-N’ present in the form of nucleic acid-N (from both plant and microbes) could be appreciable leading to a considerable overestimation of the ‘protein’ content of the silage.

scholarly journals Nucleic Acids and the Biosynthesis of Collagen

1957 ◽  
Vol 10 (3) ◽  
pp. 398 ◽  
Author(s):  
PH Springell
Keyword(s):  
Nucleic Acids ◽  
Ribonucleic Acid ◽  
Deoxyribonucleic Acid ◽  
Protein Fractions ◽  
Marked Inhibition ◽  
In Vitro Incubation ◽  
Skin Protein ◽  
Pre Treatment ◽  
Salt Fractionation

The study of skin protein fractions obtained by salt fractionation indicates that collagen is associated to a greater extent with the ribonucleoprotein fraction than with the deoxyribonucleoprotein fraction. The gelatin derived from the ribonucleoprotein fraction following in vitro incubation of foetal lambskin in the presence of glycine-2-14C was appreciably radioactive. Pre-treatment of the skin with crystalline ribonuclease resulted in a marked inhibition of glycine incorporation into total gelatin. whereas crystalline deoxyribonuclease had little effect. It is therefore concluded that ribonucleic acid rather than deoxyribonucleic acid is associated with the biosynthesis of collagen.

Some histochemical changes underlying shoot initiation in tobacco callus cultures

1970 ◽  
Vol 48 (2) ◽  
pp. 277-285 ◽  
Author(s):  
Trevor A. Thorpe ◽  
Toshio Murashige
Keyword(s):  
Nucleic Acids ◽  
Ribonucleic Acid ◽  
Acid Content ◽  
Deoxyribonucleic Acid ◽  
Histochemical Study ◽  
Callus Cultures ◽  
Tobacco Callus ◽  
Apparent Change ◽  
Shoot Initiation ◽  
Histochemical Changes

A comparative histochemical study of changes in nucleic acids, proteins, and carbohydrates was performed on freeze-substituted samples of tobacco callus, cultured under shoot-forming and non-organ-forming conditions. The DNA (deoxyribonucleic acid) content per cell showed no apparent change, whereas denser staining for RNA (ribonucleic acid) and protein were observed in shoot-forming regions just before and during the initiation of organized structures. The most dramatic finding was the heavy accumulation of starch in shoot-forming tissue; this accumulation preceded any organ formation and was mainly in regions which ultimately gave rise to primordia.

THE CONCENTRATION OF NUCLEIC ACIDS IN SOME COMMON MARINE ALGAE

1964 ◽  
Vol 42 (11) ◽  
pp. 1471-1480 ◽  
Author(s):  
E. Gordon Young
Keyword(s):  
Nucleic Acids ◽  
Ribonucleic Acid ◽  
Marine Algae ◽  
Deoxyribonucleic Acid ◽  
Exchange Resin ◽  
Anion Exchange Resin ◽  
Fucus Vesiculosus ◽  
Chondrus Crispus ◽  
Rna And Dna

The method of Smillie and Krotkov has been applied to the estimation of soluble ribonucleic acid and deoxyribonucleic acid in nine species of marine algae, representative of the Chlorophyceae, Rhodophyceae, and Phaeophyceae. Treatment of the extract with an anion exchange resin was essential for the determination of RNA. Values for RNA and DNA, expressed as percentages of total solids, were respectively as follows: Ulva lactuca 0.63 and 0.10, Chondrus crispus 0.91 and 0.65, Rhodymenia palmata 0.73 and 0.41, Porphyra umbilicalis 1.62 and 0.19, Furcellaria fastigiata 0.37 and 0.36, Laminaria longicruris 0.68 and 0.06, L. digitata 0.67 and 0.30, L. agardhii 0.90 and 0.07, Fucus vesiculosus 0.29 and 0.05, based on commercial nucleic acids assayed by ultraviolet absorption, as standards. Use of ribose and deoxyribose as standards gave lower results. Attempts to prepare pure polynucleotides from several marine algae were unsuccessful.

Anti-deoxyribonucleic acid antibody associated with persistent infection of mink with Aleutian disease virus

1980 ◽  
Vol 29 (2) ◽  
pp. 452-458
Author(s):  
E C Hahn ◽  
A J Kenyon
Keyword(s):  
Nucleic Acids ◽  
Disease Virus ◽  
Experimental Infection ◽  
Persistent Infection ◽  
Ribonucleic Acid ◽  
Deoxyribonucleic Acid ◽  
Intermediate Level ◽  
Equivalence Point ◽  
Double Stranded Dna ◽  
Aleutian Disease Virus

Anti-deoxyribonucleic acid (DNA) antibody was quantitated in sera from mink infected with Aleutian disease virus (ADV). During the course of the disease after experimental infection, the amount of anti-DNA antibody increased 60% initially, but then decreased to an intermediate level when measured 2.5 months later. The percentage of serum immunoglobulin, however, steadily increased over 3.5-fold during this period, resulting in the characteristic gammopathy. Correlation between the level of anti-DNA antibody and hypergammaglobulinemia was demonstrated with sera from chronically infected mink. Competition experiments and use of labeled nucleic acids indicated that the immunoactivity was more specific for double-stranded DNA than single-stranded DNA or ribonucleic acid. Anti-DNA antibody was found in purified immunoglobulin from chronically infected mink. Differences in avidity of antibody to DNA among antisera that had the same equivalence point were found. Avidity of antibody for DNA increased during the course of the disease.

scholarly journals Säurehydrolyse von Ribonucleinsäure und Desoxyribonucleinsäure

1961 ◽  
Vol 16 (10) ◽  
pp. 673-678 ◽  
Author(s):  
W. Pollmann ◽  
G. Schramm
Keyword(s):  
Enzymatic Hydrolysis ◽  
Nucleic Acids ◽  
Potentiometric Titration ◽  
Ribonucleic Acid ◽  
Deoxyribonucleic Acid ◽  
Potentiometric Method ◽  
Ribonucleic Acids ◽  
Rate Of Hydrolysis ◽  
Phosphate Groups

A method for the potentiometric titration of secondary phosphate groups in nucleic acids is described. Ribonucleic acids of yeast and of microsomes contain 5—6% secondary phosphate groups which cannot be removed by dialysis. The potentiometric method was applied to study several enzymatic hydrolyses and the non-enzymatic hydrolysis between pH 2.4 —1.8. The rate of hydrolysis for the purines and for the phosphate groups is approximately proportional to the H®-concentration. The constants of hydrolysis for ribonucleic acid and for deoxyribonucleic acid were determined. In DNA the depurinisation is 650 times faster than in RNA.

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