scholarly journals Nucleic Acids and the Biosynthesis of Collagen

1957 ◽  
Vol 10 (3) ◽  
pp. 398 ◽  
Author(s):  
PH Springell
Keyword(s):  
Nucleic Acids ◽  
Ribonucleic Acid ◽  
Deoxyribonucleic Acid ◽  
Protein Fractions ◽  
Marked Inhibition ◽  
In Vitro Incubation ◽  
Skin Protein ◽  
Pre Treatment ◽  
Salt Fractionation

The study of skin protein fractions obtained by salt fractionation indicates that collagen is associated to a greater extent with the ribonucleoprotein fraction than with the deoxyribonucleoprotein fraction. The gelatin derived from the ribonucleoprotein fraction following in vitro incubation of foetal lambskin in the presence of glycine-2-14C was appreciably radioactive. Pre-treatment of the skin with crystalline ribonuclease resulted in a marked inhibition of glycine incorporation into total gelatin. whereas crystalline deoxyribonuclease had little effect. It is therefore concluded that ribonucleic acid rather than deoxyribonucleic acid is associated with the biosynthesis of collagen.

Peptide synthesis in bone marrow: insulin and thyroxin effects

1962 ◽  
Vol 203 (4) ◽  
pp. 693-696 ◽  
Author(s):  
Thomas F. Necheles
Keyword(s):  
Bone Marrow ◽  
Nucleic Acid ◽  
Peptide Synthesis ◽  
Ribonucleic Acid ◽  
Deoxyribonucleic Acid ◽  
Optimum Concentration ◽  
The Other ◽  
Anabolic Effect ◽  
Oxygen Buffer

Myeloid marrow was rapidly removed from femurs of fasting young rabbits, sectioned, and incubated in Krebs-bicarbonate-CO2-oxygen buffer with appropriate C14-labeled precursors. All manipulations were designed to preserve the architecture of the tissue. After 1 hr the protein or nucleic acid-adenine was isolated and purified. Insulin, 0.01 U/ml added in vitro, stimulated histidine-2(ring)-C14 incorporation into protein by 26 ± 1.4%; alkali-treated insulin was inactive. Thyroxin elicited a 49.4 ± 2.1% stimulation at an optimum concentration of 10–7 m. Triiodothyronine, but not diiodothyronine, also had a significant effect. Insulin increased incorporation of carbon from adenosine-8-C14 into adenine of ribonucleic acid and deoxyribonucleic acid. Thyroxin, on the other hand, was without consistent effect on this process. Thyroxin stimulated significantly the incorporation of C14 of glycine-2-C14 into adenine. The possibility that part of the anabolic effect of thyroxin on bone marrow may arise from a stimulus to incorporation of precursors into purines is suggested.

Changes in the nucleic acid content of the uterus of sheep during involution following ovariectomy and following oestrogenic stimulation

1976 ◽  
Vol 27 (5) ◽  
pp. 669 ◽  
Author(s):  
DA Little ◽  
LJ Lambourne
Keyword(s):  
Nucleic Acid ◽  
Nucleic Acids ◽  
Ribonucleic Acid ◽  
Acid Content ◽  
Deoxyribonucleic Acid ◽  
Total Content ◽  
Mean Value ◽  
Nucleic Acid Content ◽  
Uterine Tissue ◽  
Rna Concentration

The concentrations of nucleic acids, deoxyribonucleic acid (DNA) and ribonucleic acid (RNA), in ovine uterine tissue were studied to determine whether the ratio RNA/DNA might be used in the bioassay of oestrogenic activity. As the uterus decreased in size with time after ovariectomy, the concentration of DNA increased markedly, but the total content of DNA in the uterus remained constant (mean value 342 ? 8 (SE) mg). The RNA concentration of the tissue decreased during involution, with the result that a highly significant reduction of 33% in the RNA/DNA ratio occurred within 2 weeks of ovariectomy. The ratio decreased further with time. Other ewes were treated for 3 days with diethylstilboestrol dipropionate (10 µg/day), commencing 2 weeks after ovariectomy; the stage of the oestrous cycle at which the ovaries were removed had no effect on the response to the oestrogen. Changes in the nucleic acid concentrations in the uteri of these ewes, and the results obtained during involution, indicate that the RNA/DNA ratio of uterine tissue in ovariectomized ewes has potential as a basis for the bioassay of materials of low oestrogenic potency.

scholarly journals Effect of denervation on the synthesis of ribonucleic acid and deoxyribonucleic acid in rat diaphragm muscle

1968 ◽  
Vol 108 (2) ◽  
pp. 177-183 ◽  
Author(s):  
K L Manchester ◽  
E J Harris
Keyword(s):  
Nucleic Acids ◽  
Ribonucleic Acid ◽  
Orotic Acid ◽  
Deoxyribonucleic Acid ◽  
Diaphragm Muscle ◽  
Unit Mass ◽  
Rat Diaphragm ◽  
The Third

1. The effect of unilateral denervation of rat diaphragm muscle on its content of nucleic acids and their incorporation of precursors was investigated. 2. After denervation the paralysed hemidiaphragm hypertrophies and within 3 days its content of RNA increases considerably. The concentration of DNA/unit mass remains fairly constant. 3. During this period there is some increase in the rate of incorporation of [14C]adenine into RNA, whereas there is some diminution in the rate of incorporation of [14C]orotic acid. 4. Incorporation of [14C]adenine and [3H]thymidine into DNA is much increased in the paralysed tissue, reaching its maximum by about the third day, but returning to normal by the tenth. 5. The significance of these results in relation to the hypertrophy after denervation is discussed.

Nucleic Acids in Grass and Grass Silage

1988 ◽  
Vol 1988 ◽  
pp. 86-86
Author(s):  
A B McAllan ◽  
G D Braithwaite
Keyword(s):  
Nucleic Acid ◽  
Nucleic Acids ◽  
Protein Content ◽  
Ribonucleic Acid ◽  
Protein Fraction ◽  
Dry Matter ◽  
Deoxyribonucleic Acid ◽  
Total N ◽  
Grass Silage ◽  
Microbial N

Little attention has been directed at defining the ‘protein’ fraction of silages. This component is normally estimated by fractionation based on solubility characteristics and under the conditions most commonly used, nucleic acids (ribonucleic acid (RNA) and deoxyribonucleic acid (DNA)) would appear in the protein fraction. Grasses and legumes can contain appreciable amounts of nucleic acids ranging from 11-29 and 19-53 g/kg dry matter respectively (McAllan, 1982). No information is available as to the effects of ensilage on these nucleic acids. Microbes also contain appreciable amounts of nucleic acids which can account for 150-200 gN/kg total-N of the cell and these amounts may vary according to the stage of growth. It has been suggested (Ullrich, 1982) that microbial-N contribution to the total-N content of silage is as much as 220-280 g/kg. Thus the total amount of silage ‘protein-N’ present in the form of nucleic acid-N (from both plant and microbes) could be appreciable leading to a considerable overestimation of the ‘protein’ content of the silage.

Template Integrity and the Activity of Eukaryotic Deoxyribonucleic Acid-Dependent Ribonucleic Acid Polymerase in vitro

1973 ◽  
Vol 1 (3) ◽  
pp. 650-655 ◽  
Author(s):  
PETER H. W. BUTTERWORTH ◽  
SARAH JANE FLINT ◽  
C. JAMES CHESTERTON
Keyword(s):  
Ribonucleic Acid ◽  
Deoxyribonucleic Acid
Sign in / Sign up

Export Citation Format

Share Document