THE CONCENTRATION OF NUCLEIC ACIDS IN SOME COMMON MARINE ALGAE

1964 ◽  
Vol 42 (11) ◽  
pp. 1471-1480 ◽  
Author(s):  
E. Gordon Young
Keyword(s):  
Nucleic Acids ◽  
Ribonucleic Acid ◽  
Marine Algae ◽  
Deoxyribonucleic Acid ◽  
Exchange Resin ◽  
Anion Exchange Resin ◽  
Fucus Vesiculosus ◽  
Chondrus Crispus ◽  
Rna And Dna

The method of Smillie and Krotkov has been applied to the estimation of soluble ribonucleic acid and deoxyribonucleic acid in nine species of marine algae, representative of the Chlorophyceae, Rhodophyceae, and Phaeophyceae. Treatment of the extract with an anion exchange resin was essential for the determination of RNA. Values for RNA and DNA, expressed as percentages of total solids, were respectively as follows: Ulva lactuca 0.63 and 0.10, Chondrus crispus 0.91 and 0.65, Rhodymenia palmata 0.73 and 0.41, Porphyra umbilicalis 1.62 and 0.19, Furcellaria fastigiata 0.37 and 0.36, Laminaria longicruris 0.68 and 0.06, L. digitata 0.67 and 0.30, L. agardhii 0.90 and 0.07, Fucus vesiculosus 0.29 and 0.05, based on commercial nucleic acids assayed by ultraviolet absorption, as standards. Use of ribose and deoxyribose as standards gave lower results. Attempts to prepare pure polynucleotides from several marine algae were unsuccessful.

Determination of α-Hydroxy Acids in Foods by TLC and Manometric Measurement

1970 ◽  
Vol 53 (3) ◽  
pp. 621-623
Author(s):  
M Trop ◽  
I M Levinger
Keyword(s):  
Thin Layer ◽  
Quantitative Determination ◽  
Exchange Resin ◽  
Anion Exchange Resin ◽  
Hydroxy Acids ◽  
Keto Acids ◽  
Alcoholic Extraction ◽  
Layer Chromatography ◽  
Manometric Measurement

Abstract An analytical method for determination of α-hydroxy and α-keto acids is developed based on separation of the acids from other components by acidic alcoholic extraction and by adsorption on and elution from an anion exchange resin, followed by thin layer chromatography. Acids are identified by eerie ammonium nitrate reagent sprayed on the chromatogram. Quantitative determination is performed by manometric measurement of gas released in reaction with that reagent.

Changes in the nucleic acid content of the uterus of sheep during involution following ovariectomy and following oestrogenic stimulation

1976 ◽  
Vol 27 (5) ◽  
pp. 669 ◽  
Author(s):  
DA Little ◽  
LJ Lambourne
Keyword(s):  
Nucleic Acid ◽  
Nucleic Acids ◽  
Ribonucleic Acid ◽  
Acid Content ◽  
Deoxyribonucleic Acid ◽  
Total Content ◽  
Mean Value ◽  
Nucleic Acid Content ◽  
Uterine Tissue ◽  
Rna Concentration

The concentrations of nucleic acids, deoxyribonucleic acid (DNA) and ribonucleic acid (RNA), in ovine uterine tissue were studied to determine whether the ratio RNA/DNA might be used in the bioassay of oestrogenic activity. As the uterus decreased in size with time after ovariectomy, the concentration of DNA increased markedly, but the total content of DNA in the uterus remained constant (mean value 342 ? 8 (SE) mg). The RNA concentration of the tissue decreased during involution, with the result that a highly significant reduction of 33% in the RNA/DNA ratio occurred within 2 weeks of ovariectomy. The ratio decreased further with time. Other ewes were treated for 3 days with diethylstilboestrol dipropionate (10 µg/day), commencing 2 weeks after ovariectomy; the stage of the oestrous cycle at which the ovaries were removed had no effect on the response to the oestrogen. Changes in the nucleic acid concentrations in the uteri of these ewes, and the results obtained during involution, indicate that the RNA/DNA ratio of uterine tissue in ovariectomized ewes has potential as a basis for the bioassay of materials of low oestrogenic potency.

Quantitative Determination of FD&C Colors in Foods

1975 ◽  
Vol 58 (2) ◽  
pp. 278-282
Author(s):  
Charles Graichen
Keyword(s):  
Quantitative Determination ◽  
Anion Exchange ◽  
Salt Solution ◽  
Exchange Resin ◽  
Anion Exchange Resin ◽  
Collaborative Study ◽  
Chromatographic Technique ◽  
Different Color ◽  
Selection Of

Abstract A published procedure using Amberlite LA-2 (a liquid anion exchange resin) solutions to extract colors from food and the salt solution cellulose chromatographic technique to separate colors from each other were updated and submitted to collaborative study. Three batches of cookies and 4 purchased products were analyzed by 13 collaborators. The 3 batches of cookies contained different color mixtures requiring the selection of various procedures. The 4 purchased products were selected principally to include a variety of foods. Some results were partially deficient. The deficiencies reflect certain inadequacies in the directions as written rather than basic flaws in the method. Some improvements were suggested by the collaborators. The Associate Referee recommends that the method be further revised to correct the present inadequacies and to include some improvements, and that the revised method be submitted to another collaborative study.

Liquid Chromatographic Determination of Melamine in Beverages

1987 ◽  
Vol 70 (3) ◽  
pp. 457-460 ◽  
Author(s):  
Hajimu Ishiwata ◽  
Takiko Inoue ◽  
Takeshi Yamazaki ◽  
Kunitoshi Yoshihira
Keyword(s):  
Exchange Resin ◽  
Anion Exchange Resin ◽  
Chromatographic Determination ◽  
Formaldehyde Resin ◽  
Melamine Formaldehyde ◽  
Quantitation Limit ◽  
Liquid Chromatographic Method ◽  
Liquid Chromatographic Determination ◽  
Liquid Chromatographic

Abstract A liquid chromatographic method is described for the determination of melamine in beverages. Melamine is separated by column chromatography using cation and anion exchange resin and determined by ion-pair liquid chromatography using an ODS column and a mixture of acetonitrile and 0.05M phosphate buffer (pH 3.0) containing 0.005M sodium 1-laurylsulfate (1 + 4, v/v) as mobile phase. Recoveries of melamine ranged between 90.3 ± 7.8 and 102.1 ± 5.6% at levels of 0.6 to 2.4 ppm in 4 kinds of beverages. The quantitation limit was 2.5 μg melamine in 50 mL beverage. The method was applied to the migration test of melamine from melamine-formaldehyde resin products to the beverages.

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