Efficient Gene Transfection Using Novel Cationic Polymers Poly(hydroxyalkylene imines)

2010 ◽  
Vol 21 (9) ◽  
pp. 1602-1611 ◽  
Author(s):  
Lolita Zaliauskiene ◽  
Ula Bernadisiute ◽  
Ausvydas Vareikis ◽  
Ricardas Makuska ◽  
Ieva Volungeviciene ◽  
...  
Keyword(s):  
Gene Transfection ◽  
Cationic Polymers ◽  
Efficient Gene

scholarly journals Biopolymeric Materials Used as Nonviral Vectors: A Review

2021 ◽  
Vol 2 (1) ◽  
pp. 100-109
Author(s):  
Jailson de Araújo Santos ◽  
Daniel Barbosa Liarte ◽  
Alessandra Braga Ribeiro ◽  
Marcia dos Santos Rizzo ◽  
Marcília Pinheiro da Costa ◽  
...  
Keyword(s):  
Chemical Structure ◽  
Web Of Science ◽  
Gene Transfection ◽  
Genetic Material ◽  
Nonviral Vectors ◽  
Cationic Polymers ◽  
Genetic Trait ◽  
Low Diversity ◽  
Different Types ◽  
Materials Used

Bacterial transformation and gene transfection can be understood as being the results of introducing specific genetic material into cells, resulting in gene expression, and adding a new genetic trait to the host cell. Many studies have been carried out to investigate different types of lipids and cationic polymers as promising nonviral vectors for DNA transfer. The present study aimed to carry out a systematic review on the use of biopolymeric materials as nonviral vectors. The methodology was carried out based on searches of scientific articles and applications for patents published or deposited from 2006 to 2020 in different databases for patents (EPO, USPTO, and INPI) and articles (Scopus, Web of Science, and Scielo). The results showed that there are some deposits of patents regarding the use of chitosan as a gene carrier. The 16 analyzed articles allowed us to infer that the use of biopolymers as nonviral vectors is limited due to the low diversity of biopolymers used for these purposes. It was also observed that the use of different materials as nonviral vectors is based on chemical structure modifications of the material, mainly by the addition of cationic groups. Thus, the use of biopolymers as nonviral vectors is still limited to only a few polysaccharide types, emphasizing the need for further studies involving the use of different biopolymers in processes of gene transfer.

scholarly journals Chitosan-conjugated lipid microbubble combined with ultrasound for efficient gene transfection

2018 ◽  
Vol 32 (4) ◽  
pp. 982-987 ◽  
Author(s):  
Hui Zhang ◽  
Jiawei Tu ◽  
Yangying Liao ◽  
Kuan Cai ◽  
Yue Li ◽  
...  
Keyword(s):  
Gene Transfection ◽  
Efficient Gene

scholarly journals Ionic liquids with thioether motifs as synthetic cationic lipids for gene delivery

2017 ◽  
Vol 53 (59) ◽  
pp. 8328-8331 ◽  
Author(s):  
Jamie C. Gaitor ◽  
Lauren M. Paul ◽  
Melissa M. Reardon ◽  
Taha Hmissa ◽  
Samuel Minkowicz ◽  
...  
Keyword(s):  
Ionic Liquids ◽  
Gene Delivery ◽  
Click Chemistry ◽  
Gene Transfection ◽  
Cationic Lipids ◽  
Efficient Gene

Novel lipidic ionic liquids with imidazolium headgroups (red), thioether linkers (black), and two hydrophobic tails (blue) as efficient gene transfection vectors, synthesized via thiol–yne click chemistry.

Functionalized Scaffold for in Situ Efficient Gene Transfection of Mesenchymal Stem Cells Spheroids toward Chondrogenesis

2018 ◽  
Vol 10 (40) ◽  
pp. 33993-34004 ◽  
Author(s):  
Kunxi Zhang ◽  
Haowei Fang ◽  
Yechi Qin ◽  
Lili Zhang ◽  
Jingbo Yin
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Gene Transfection ◽  
Efficient Gene

A PEG-b-poly(disulfide-l-lysine) based redox-responsive cationic polymer for efficient gene transfection

2019 ◽  
Vol 7 (11) ◽  
pp. 1893-1905 ◽  
Author(s):  
Ihsan Ullah ◽  
Jing Zhao ◽  
Shah Rukh ◽  
Khan Muhammad ◽  
Jintang Guo ◽  
...  
Keyword(s):  
Gene Transfection ◽  
Cationic Polymer ◽  
Endosomal Escape ◽  
Gene Vector ◽  
Efficient Gene ◽  
Redox Responsive ◽  
Responsive Gene

A redox responsive gene vector efficiently delivered pDNA with a good internalization tendency, efficient endosomal escape and high nucleus internalization.

scholarly journals Cyclopropenium Nanoparticles and Gene Transfection in Cells

Pharmaceutics ◽  
2020 ◽  
Vol 12 (8) ◽  
pp. 768
Author(s):  
Noam Y. Steinman ◽  
Luis M. Campos ◽  
Yakai Feng ◽  
Abraham J. Domb ◽  
Hossein Hosseinkhani
Keyword(s):  
Viral Vectors ◽  
Gene Transfection ◽  
Medical Science ◽  
Genetic Material ◽  
Mouse Fibroblast ◽  
Ethylene Imine ◽  
Efficient Gene ◽  
Poly Ethylene ◽  
First Time

Non-viral vectors for the transfection of genetic material are at the frontier of medical science. In this article, we introduce for the first time, cyclopropenium-containing nanoparticles as a cationic carrier for gene transfection, as an alternative to the common quaternary ammonium transfection agents. Cyclopropenium-based cationic nanoparticles were prepared by crosslinking poly(ethylene imine) (PEI) with tetrachlorocyclopropene. These nanoparticles were electrostatically complexed with plasmid DNA into nanoparticles (~50 nm). Their cellular uptake into F929 mouse fibroblast cells, and their eventual expression in vitro have been described. Transfection is enhanced relative to PEI with minimal toxicity. These cyclopropenium nanoparticles possess efficient gene transfection capabilities with minimal cytotoxicity, which makes them novel and promising candidates for gene therapy.

α-Tocopherol derived lipid dimers as efficient gene transfection agents. Mechanistic insights into lipoplex internalization and therapeutic induction of apoptotic activity

2015 ◽  
Vol 13 (8) ◽  
pp. 2444-2452 ◽  
Author(s):  
Krishan Kumar ◽  
Bappa Maiti ◽  
Paturu Kondaiah ◽  
Santanu Bhattacharya
Keyword(s):  
Cell Lines ◽  
Cancer Cell ◽  
Gene Transfection ◽  
Dna Delivery ◽  
Cancer Cell Lines ◽  
Efficient Gene ◽  
Apoptotic Activity

Dimeric cationic tocopheryl lipids for efficacious therapeutic pDNA delivery in cancer cell lines.

Dual-functionalized calcium carbonate based gene delivery system for efficient gene delivery

RSC Advances ◽  
2014 ◽  
Vol 4 (73) ◽  
pp. 38623-38629 ◽  
Author(s):  
Chao-Qun Wang ◽  
Meng-Qing Gong ◽  
Jin-Long Wu ◽  
Ren-Xi Zhuo ◽  
Si-Xue Cheng
Keyword(s):  
Calcium Carbonate ◽  
Gene Delivery ◽  
Delivery System ◽  
Gene Transfection ◽  
Cell Penetrating Peptide ◽  
Gene Delivery System ◽  
Dna Nanoparticles ◽  
Efficient Gene ◽  
Cell Penetrating ◽  
A Cell

Dual-functionalized KALA/PS/CaCO3/DNA nanoparticles containing a cell penetrating peptide (KALA) and protamine sulfate (PS) could effectively mediate gene transfection at a low DNA concentration.

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