α-Tocopherol derived lipid dimers as efficient gene transfection agents. Mechanistic insights into lipoplex internalization and therapeutic induction of apoptotic activity

2015 ◽  
Vol 13 (8) ◽  
pp. 2444-2452 ◽  
Author(s):  
Krishan Kumar ◽  
Bappa Maiti ◽  
Paturu Kondaiah ◽  
Santanu Bhattacharya
Keyword(s):  
Cell Lines ◽  
Cancer Cell ◽  
Gene Transfection ◽  
Dna Delivery ◽  
Cancer Cell Lines ◽  
Efficient Gene ◽  
Apoptotic Activity

Dimeric cationic tocopheryl lipids for efficacious therapeutic pDNA delivery in cancer cell lines.

Synthesis, screening and pro-apoptotic activity of novel acyl spermidine derivatives on human cancer cell lines

2017 ◽  
Vol 93 ◽  
pp. 190-201 ◽  
Author(s):  
Syed Shoeb Razvi ◽  
Hani Choudhry ◽  
Said Salama Moselhy ◽  
Taha Abduallah Kumosani ◽  
Mohammed Nihal Hasan ◽  
...  
Keyword(s):  
Cell Lines ◽  
Cancer Cell ◽  
Human Cancer ◽  
Cancer Cell Lines ◽  
Human Cancer Cell ◽  
Human Cancer Cell Lines ◽  
Apoptotic Activity

scholarly journals New Paracyclophanylthiazoles with Anti-Leukemia Activity: Design, Synthesis, Molecular Docking, and Mechanistic Studies

Molecules ◽  
2020 ◽  
Vol 25 (13) ◽  
pp. 3089 ◽  
Author(s):  
Ashraf A Aly ◽  
Stefan Bräse ◽  
Alaa A. Hassan ◽  
Nasr K. Mohamed ◽  
Lamiaa E. Abd El-Haleem ◽  
...  
Keyword(s):  
Cell Lines ◽  
Cancer Cell ◽  
Annexin V ◽  
Cancer Cell Lines ◽  
Multidrug Resistant ◽  
Docking Studies ◽  
Mechanistic Study ◽  
Design Synthesis ◽  
Apoptotic Activity ◽  
Rpmi 8226

A new series of methyl 2-(2-(4′-[2.2]paracyclophanyl)-hydrazinylidene)-3-substituted-4-oxothiazolidin-5-ylidene)acetates 3a–f were synthesized from the reaction of paracyclophanyl-acylthiosemicarbazides 2a–f with dimethyl acetylenedicarboxylate. Based upon nuclear magnetic resonance (NMR), infrared (IR), and mass spectra (HRMS), the structure of the obtained products was elucidated. X-ray structure analysis was also used as unambiguous tool to elucidate the structure of the products. The target compounds 3a–f were screened against 60 cancer cell lines. They displayed anticancer activity against a leukemia subpanel, namely, RPMI-8226 and SR cell lines. The activity of compound 3a was found as the most cytotoxic potency against 60 cancer cell lines. Consequently, it was selected for further five doses analysis according to National Cancer Institute (NCI) protocol. The cytotoxic effect showed selectivity ratios ranging between 0.63 and 1.28 and between 0.58 and 5.89 at the GI50 and total growth inhibition (TGI) levels, respectively. Accordingly, compound 3a underwent further mechanistic study against the most sensitive leukemia RPMI-8226 and SR cell lines. It showed antiproliferation with IC50 = 1.61 ± 0.04 and 1.11 ± 0.03 µM against RPMI-8226 and SR cell lines, respectively. It also revealed a remarkable tubulin inhibitory activity, compared to colchicine with IC50 = 4.97 µM/mL. Caspase-3, BAX, and Bcl-2 assays for 3a using annexin V-FITC staining revealed significant pro-apoptotic activity. Furthermore, multidrug-resistant leukemia SR cells were used to show better resistance indices (1.285 ng/mL, 1.15-fold) than the reference. Docking studies with β-tubulin indicate that most of the tested compounds illustrated good binding at the colchicine binding site of the enzyme, especially for compound 3a, which made several interactions better than that of the reference colchicine.

Cytotoxicity and apoptotic activity of novel organobismuth(V) and organoantimony(V) complexes in different cancer cell lines

2016 ◽  
Vol 109 ◽  
pp. 254-267 ◽  
Author(s):  
Arshad Islam ◽  
Bernardo Lages Rodrigues ◽  
Ivana Marques Marzano ◽  
Elene C. Perreira-Maia ◽  
Dalton Dittz ◽  
...  
Keyword(s):  
Cell Lines ◽  
Cancer Cell ◽  
Cancer Cell Lines ◽  
Apoptotic Activity

Antitumor activity of three mouse mammary cancer cell lines after interferon-gamma gene transfection*

Surgery ◽  
1995 ◽  
Vol 118 (2) ◽  
pp. 251-256 ◽  
Author(s):  
Y MATORY ◽  
M CHEN ◽  
D DORFMAN ◽  
A WILLIAMS ◽  
P GOEDEGEBUURE ◽  
...  
Keyword(s):  
Antitumor Activity ◽  
Cell Lines ◽  
Cancer Cell ◽  
Interferon Gamma ◽  
Mammary Cancer ◽  
Gene Transfection ◽  
Cancer Cell Lines ◽  
Mammary Cancer Cell

scholarly journals Analysis of the anti-cancer effect of ethyl-p-methoxycinnamate extracted cekur (Kaempferia galanga) on cancer cell lines with wild- type and null p53

2020 ◽  
Vol 1 (1) ◽  
pp. 28-33
Author(s):  
Solachuddin Jauhari Arief Ichwan ◽  
Syahirah Sazeli ◽  
Widya Lestari
Keyword(s):  
Cell Lines ◽  
Cancer Cell ◽  
Cancer Cell Lines ◽  
Major Constituent ◽  
Wild Type ◽  
P53 Pathway ◽  
Kaempferia Galanga ◽  
Anti Cancer ◽  
Apoptotic Activity

This study aimed to examine the in-vitro anti-cancer potential of ethyl-p-methoxycinnamate (EPMC), the major constituent of Kaempferia galanga (K. galanga) in selected human lung adenocarcinoma cells line A549 (p53 wild-type) and H1299 (p53 null). The involvement of p53 pathway in the anti-cancer effect of EPMC on selected cells was determined using MTT assay and Real-time PCR. The MTT results show that EPMC induces cytotoxicity in a dose- dependent manner in A549 cancer cell lines containing the p53 wild-type gene. Meanwhile, our RT-PCR results indicate that the apoptotic activity of EPMC does not involve the p53 pathway. Overall, these results indicate that EPMC compounds of K. galanga stimulates in vitro cytotoxic and apoptotic activity unrelated to the p53 pathway

Synthesis and apoptotic activity of new pyrazole derivatives in cancer cell lines

2015 ◽  
Vol 23 (17) ◽  
pp. 5799-5808 ◽  
Author(s):  
George Mihai Nitulescu ◽  
Constantin Draghici ◽  
Octavian Tudorel Olaru ◽  
Lilia Matei ◽  
Aldea Ioana ◽  
...  
Keyword(s):  
Cell Lines ◽  
Cancer Cell ◽  
Cancer Cell Lines ◽  
Pyrazole Derivatives ◽  
Apoptotic Activity
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