A PEG-b-poly(disulfide-l-lysine) based redox-responsive cationic polymer for efficient gene transfection

2019 ◽  
Vol 7 (11) ◽  
pp. 1893-1905 ◽  
Author(s):  
Ihsan Ullah ◽  
Jing Zhao ◽  
Shah Rukh ◽  
Khan Muhammad ◽  
Jintang Guo ◽  
...  
Keyword(s):  
Gene Transfection ◽  
Cationic Polymer ◽  
Endosomal Escape ◽  
Gene Vector ◽  
Efficient Gene ◽  
Redox Responsive ◽  
Responsive Gene

A redox responsive gene vector efficiently delivered pDNA with a good internalization tendency, efficient endosomal escape and high nucleus internalization.

scholarly journals Ar-23 Derivatives With High Endosomal Disrupting Ability Enhance Poly(L-lysine)-mediated Gene Transfection

2020 ◽  
Author(s):  
Shikun Zhang ◽  
Lin Gong ◽  
Xue Zhang ◽  
Zhi-min Yun ◽  
Su-Bo Li ◽  
...  
Keyword(s):  
Transfection Efficiency ◽  
Gene Transfection ◽  
Physicochemical Characteristics ◽  
Cationic Polymer ◽  
Ph Sensitive ◽  
Lytic Activity ◽  
Endosomal Escape ◽  
Charged Residues ◽  
Low Cytotoxicity ◽  
Positively Charged

Abstract Background：pH-sensitive peptides are a relatively new strategy for conquering poor endosomal release of cationic polymer-mediated transfection. Modification of antimicrobial peptides (AMPs) by exchanging positively-charged residues with negatively-charged glutamic acid residues (Glu) greatly improved its lytic activity at the endosomal pH, which could improve cationic polymer-mediated transfection.Methods：In the present study, we investigate the effect of the number of Glu substituted for positively-charged residues on the endosomal escape activity of AR-23 and the ability of mutated AR-23 for enhancing cationic polymer-mediated transfection. Three analogs were synthesized by replacing the positively-charged residues in AR-23's sequence with Glu one-by-one. The pH-sensitive lysis ability of the peptides, the effect of peptides on the physicochemical characteristics, the intracellular trafficking，the transfection efficiency, and the cytotoxicity of the polyplexes were determined.Results：Increased lytic activity of peptides was observed with the increased number of Glu replacement in the AR-23 sequence at acidic pH. The number of Glu substituted for positively-charged residues of AR-23 dramatically affects its lysis ability at neutral pH. Triple-Glu substitution in AR-23's sequence greatly improved PLL-mediated gene transfection efficiency while maintaining low cytotoxicity.Conclusion：The results indicate that replacement of positively-charged residues with enough Glu residues may be considered a method for designing pH-sensitive peptides, which could be applied as potential enhancers for improving cationic polymer-mediated transfection.

Polyethyleneimine-grafted poly(N-3-hydroxypropyl)aspartamide as a biodegradable gene vector for efficient gene transfection

Soft Matter ◽  
2010 ◽  
Vol 6 (5) ◽  
pp. 955 ◽  
Author(s):  
Dan Chen ◽  
Yuan Ping ◽  
Guping Tang ◽  
Jun Li
Keyword(s):  
Gene Transfection ◽  
Gene Vector ◽  
Efficient Gene

Construction of traceable cucurbit[7]uril-based virus-mimicking quaternary complexes with aggregation-induced emission for efficient gene transfection

2020 ◽  
Vol 8 (33) ◽  
pp. 7475-7482
Author(s):  
De-E Liu ◽  
Xiangjie Yan ◽  
Jinxia An ◽  
Jianbiao Ma ◽  
Hui Gao
Keyword(s):  
Gene Delivery ◽  
Delivery System ◽  
Gene Transfection ◽  
Delivery Systems ◽  
Endosomal Escape ◽  
Gene Delivery System ◽  
Aggregation Induced Emission ◽  
Efficient Gene ◽  
Novel Virus

A novel virus-mimicking gene delivery system with excellent endosomal escape, efficient transfection, and traceability is developed which can serve as a reference for the construction of novel multifunctional efficient gene delivery systems.

Efficacious redox-responsive gene delivery in serum by ferrocenylated monomeric and dimeric cationic cholesterols

2015 ◽  
Vol 13 (14) ◽  
pp. 4310-4320 ◽  
Author(s):  
Gururaja Vulugundam ◽  
Krishan Kumar ◽  
Paturu Kondaiah ◽  
Santanu Bhattacharya
Keyword(s):  
Gene Delivery ◽  
Gene Transfection ◽  
Redox Active ◽  
Redox Responsive ◽  
Responsive Gene

New redox-active monomeric and dimeric ferrocenylated cationic cholesterols for gene transfection.

Efficient Gene Transfection by Histidine-Modified Chitosan through Enhancement of Endosomal Escape

2010 ◽  
Vol 21 (6) ◽  
pp. 1087-1095 ◽  
Author(s):  
Kai-Ling Chang ◽  
Yuriko Higuchi ◽  
Shigeru Kawakami ◽  
Fumiyoshi Yamashita ◽  
Mitsuru Hashida
Keyword(s):  
Gene Transfection ◽  
Endosomal Escape ◽  
Modified Chitosan ◽  
Efficient Gene

scholarly journals Gold Nanocluster Decorated Polypeptide/DNA Complexes for NIR Light and Redox Dual-Responsive Gene Transfection

Molecules ◽  
2016 ◽  
Vol 21 (8) ◽  
pp. 1103 ◽  
Author(s):  
Qi Lei ◽  
Jing-Jing Hu ◽  
Lei Rong ◽  
Han Cheng ◽  
Yun-Xia Sun ◽  
...  
Keyword(s):  
Gene Transfection ◽  
Dna Complexes ◽  
Gold Nanocluster ◽  
Dual Responsive ◽  
Nir Light ◽  
Responsive Gene

scholarly journals Aliphatic Quaternary Ammonium Functionalized Nanogels for Gene Delivery

Pharmaceutics ◽  
2021 ◽  
Vol 13 (11) ◽  
pp. 1964
Author(s):  
Huaiying Zhang ◽  
Damla Keskin ◽  
Willy H. de Haan-Visser ◽  
Guangyue Zu ◽  
Patrick van Rijn ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Tertiary Amine ◽  
Quaternary Ammonium ◽  
Gene Transfection ◽  
Genetic Material ◽  
Cationic Lipid ◽  
Endosomal Escape ◽  
Hereditary Diseases ◽  
Viral Gene

Gene therapy is a promising treatment for hereditary diseases, as well as acquired genetic diseases, including cancer. Facing the complicated physiological and pathological environment in vivo, developing efficient non-viral gene vectors is needed for their clinical application. Here, poly(N-isopropylacrylamide) (p(NIPAM)) nanogels are presented with either protonatable tertiary amine groups or permanently charged quaternized ammonium groups to achieve DNA complexation ability. In addition, a quaternary ammonium-functionalized nanogel was further provided with an aliphatic moiety using 1-bromododecane to add a membrane-interacting structure to ultimately facilitate intracellular release of the genetic material. The ability of the tertiary amine-, quaternized ammonium-, and aliphatic quaternized ammonium-functionalized p(NIPAM) nanogels (i.e., NGs, NGs-MI, and NGs-BDD, respectively) to mediate gene transfection was evaluated by fluorescence microscopy and flow cytometry. It is observed that NGs-BDD/pDNA complexes exhibit efficient gene loading, gene protection ability, and intracellular uptake similar to that of NGs-MI/pDNA complexes. However, only the NGs-BDD/pDNA complexes show a notable gene transfer efficiency, which can be ascribed to their ability to mediate DNA escape from endosomes. We conclude that NGs-BDD displays a cationic lipid-like behavior that facilitates endosomal escape by perturbing the endosomal/lysosomal membrane. These findings demonstrate that the presence of aliphatic chains within the nanogel is instrumental in accomplishing gene delivery, which provides a rationale for the further development of nanogel-based gene delivery systems.

scholarly journals Chitosan-conjugated lipid microbubble combined with ultrasound for efficient gene transfection

2018 ◽  
Vol 32 (4) ◽  
pp. 982-987 ◽  
Author(s):  
Hui Zhang ◽  
Jiawei Tu ◽  
Yangying Liao ◽  
Kuan Cai ◽  
Yue Li ◽  
...  
Keyword(s):  
Gene Transfection ◽  
Efficient Gene

scholarly journals Ionic liquids with thioether motifs as synthetic cationic lipids for gene delivery

2017 ◽  
Vol 53 (59) ◽  
pp. 8328-8331 ◽  
Author(s):  
Jamie C. Gaitor ◽  
Lauren M. Paul ◽  
Melissa M. Reardon ◽  
Taha Hmissa ◽  
Samuel Minkowicz ◽  
...  
Keyword(s):  
Ionic Liquids ◽  
Gene Delivery ◽  
Click Chemistry ◽  
Gene Transfection ◽  
Cationic Lipids ◽  
Efficient Gene

Novel lipidic ionic liquids with imidazolium headgroups (red), thioether linkers (black), and two hydrophobic tails (blue) as efficient gene transfection vectors, synthesized via thiol–yne click chemistry.

Sign in / Sign up

Export Citation Format

Share Document