scholarly journals Highly Sensitive Fluoro-Immunosensing for Biomarker Detection Using an Automatic Pipette Tip-Type Biosensing System

ACS Omega ◽  
2019 ◽  
Vol 4 (1) ◽  
pp. 1487-1493 ◽  
Author(s):  
Eri Takano ◽  
Nobuaki Shimura ◽  
Yoshihisa Ujima ◽  
Hirobumi Sunayama ◽  
Yukiya Kitayama ◽  
...  
Keyword(s):  
Biomarker Detection ◽  
Highly Sensitive ◽  
Pipette Tip ◽  
Biosensing System

scholarly journals Metal Nanoparticle and Quantum Dot Tags for Signal Amplification in Electrochemical Immunosensors for Biomarker Detection

Chemosensors ◽  
2021 ◽  
Vol 9 (4) ◽  
pp. 85
Author(s):  
Anton Popov ◽  
Benediktas Brasiunas ◽  
Asta Kausaite-Minkstimiene ◽  
Almira Ramanaviciene
Keyword(s):  
Signal Amplification ◽  
Electrochemical Immunosensor ◽  
Metal Nanoparticle ◽  
Biomarker Detection ◽  
Analytical Instruments ◽  
Electrochemical Immunosensors ◽  
Highly Sensitive ◽  
Recent Developments ◽  
Electrochemical Signal ◽  
Detection Of Biomarkers

With the increasing importance of healthcare and clinical diagnosis, as well as the growing demand for highly sensitive analytical instruments, immunosensors have received considerable attention. In this review, electrochemical immunosensor signal amplification strategies using metal nanoparticles (MNPs) and quantum dots (Qdots) as tags are overviewed, focusing on recent developments in the ultrasensitive detection of biomarkers. MNPs and Qdots can be used separately or in combination with other nanostructures, while performing the function of nanocarriers, electroactive labels, or catalysts. Thus, different functions of MNPs and Qdots as well as recent advances in electrochemical signal amplification are discussed. Additionally, the methods most often used for antibody immobilization on nanoparticles, immunoassay formats, and electrochemical methods for indirect biomarker detection are overviewed.

Highly Sensitive and Specific Zika Virus Serological Assays Using a Magnetic Modulation Biosensing System

2018 ◽  
Vol 219 (7) ◽  
pp. 1035-1043 ◽  
Author(s):  
Yehudit Michelson ◽  
Yaniv Lustig ◽  
Shira Avivi ◽  
Eli Schwartz ◽  
Amos Danielli
Keyword(s):  
Zika Virus ◽  
Highly Sensitive ◽  
Biosensing System

Multiplexed homogeneous digital immunoassay based on single-particle motion analysis

Lab on a Chip ◽  
2020 ◽  
Vol 20 (12) ◽  
pp. 2113-2121 ◽  
Author(s):  
Kenji Akama ◽  
Hiroyuki Noji
Keyword(s):  
Motion Analysis ◽  
Single Particle ◽  
Analytical Method ◽  
Particle Motion ◽  
Protein Detection ◽  
Biomarker Detection ◽  
Multiple Protein ◽  
Highly Sensitive ◽  
Simple Protocol ◽  
Single Particle Motion

Homogeneous digital immunoassay is a powerful analytical method for highly sensitive biomarker detection with a simple protocol. By using this method, we demonstrated the simultaneous multiple protein detection.

Label-free brain injury biomarker detection based on highly sensitive large area organic thin film transistor with hybrid coupling layer

2014 ◽  
Vol 5 (1) ◽  
pp. 416-426 ◽  
Author(s):  
Weiguo Huang ◽  
Kalpana Besar ◽  
Rachel LeCover ◽  
Pratima Dulloor ◽  
Jasmine Sinha ◽  
...  
Keyword(s):  
Thin Film ◽  
Brain Injury ◽  
Thin Film Transistor ◽  
Biomarker Detection ◽  
Label Free ◽  
Organic Thin Film ◽  
Organic Thin Film Transistor ◽  
Large Area ◽  
Hybrid Coupling ◽  
Highly Sensitive

Fast-Response Smart Self-Assembling Biosensors for Biomarker Detection

2007 ◽  
Author(s):  
James C. K. Lai ◽  
Marco P. Schoen ◽  
Arya Ebrahimpour ◽  
Alok Bhushan ◽  
Christopher K. Daniels ◽  
...  
Keyword(s):  
Functional Characterization ◽  
High Sensitivity ◽  
Fast Response ◽  
Biomarker Detection ◽  
Dual Purpose ◽  
Self Assembling ◽  
Highly Sensitive ◽  
Multiple Biomarkers ◽  
The One ◽  
The Ideal

The development of biosensors has been astronomical with the advent of the rapid growth of nanomaterials and nanotechnology. Nanobiosensors are becoming ubiquitous in numerous biomedical applications. Thus, there is a great impetus to exploit smart nanoparticles and other nanomaterials for designing and fabricating smart nanobiosensors that are ultrasensitive and biocompatible. We are developing smart self-assembling biosensors that can detect specific biomolecules (e.g., enzymes, cofactors, metabolites, drugs, hormones, etc.) from micro- to nanomolar levels. Applications of the biosensors include detection of organ dysfunction and/or failure (e.g., liver malfunction, heart failure, etc.), early detection of malignant cancers, toxicant identification, and other biomarkers of diseases. Although nanobiosensors that possess high sensitivity and specificity have been designed and marketed, one fundamental issue remains to be resolved. This important issue is one concerning biocompatibility. Thus, in our development of smart biosensors using nanomaterials, we have adopted a dual purpose approach. (i) On the one hand, it is necessary to systematically and comprehensively evaluate the material properties, characterize and model the signal sensing ability, and determine the biocompatibility of materials to be employed for the design of nanobiosensors. (ii) On the other hand, it is imperative to identify the ideal criteria for the designs of fast-response smart self-assembling nanobiosensors for biomarker detection. Based on a critical review of the literature and consideration of the biocompatibility, functional characterization, and other related issues discussed above, we have identify a set of criteria for the design of fast-response smart self-assembling nanobiosensors for detection of multiple biomarkers. We have also identified many biomedical areas where such nanobiosensors can be applied to detect biomarkers for various diseases. Our dual purpose approach will ultimately lead to the design of much more biocompatible and highly sensitive nanobiosensors and diagnostic equipment (nanobiosensor arrays).

Highly Sensitive Determination of Bisphenol A in Bottled Water Samples by HPLC after Its Extraction by a Novel Th-MOF Pipette-Tip Micro-SPE

2019 ◽  
Vol 58 (4) ◽  
pp. 373-382 ◽  
Author(s):  
Massoud Kaykhaii ◽  
Eilnaz Yavari ◽  
Ghasem Sargazi ◽  
Ahmad Khajeh Ebrahimi
Keyword(s):  
Bisphenol A ◽  
Water Samples ◽  
High Performance ◽  
Solid Phase ◽  
Metal Organic Framework ◽  
Sample Volume ◽  
Linear Calibration ◽  
Highly Sensitive ◽  
Metal Organic ◽  
Pipette Tip

Abstract In this study, a novel thorium metal organic framework was synthesized, characterized and used as a sorbent for very efficient pipette tip micro solid-phase extraction of bisphenol A in bottled drinking water samples using high-performance liquid chromatography as detecting instrument. Parameters which influence extraction efficiency such as pH, sample volume, amount of sorbent, type and volume of eluent, number of aspirating and dispensing cycles for extraction and elution, and volume of the sample solution were studied and optimized. A linear calibration curve was obtained in the range of 0.002–0.456 ng mL−1 (r2 = 0.996) with a detection limit of 0.0010 ng mL−1. Repeatability of batch-to-batch extraction was better than 5.0% and a reproducibility of 3.2% for real samples obtained.

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