Cellular Uptake and Cytotoxicity of Varying Aspect Ratios of Gold Nanorods in HeLa Cells

2020 ◽  
Vol 3 (3) ◽  
pp. 1374-1384 ◽  
Author(s):  
Deshani Fernando ◽  
Shoukath Sulthana ◽  
Yolanda Vasquez
Keyword(s):  
Cellular Uptake ◽  
Gold Nanorods ◽  
Hela Cells ◽  
Aspect Ratios

Mechanism for the Cellular Uptake of Targeted Gold Nanorods of Defined Aspect Ratios

Small ◽  
2016 ◽  
Vol 12 (37) ◽  
pp. 5178-5189 ◽  
Author(s):  
Hongrong Yang ◽  
Zhong Chen ◽  
Lei Zhang ◽  
Wing-Yin Yung ◽  
Ken Cham-Fai Leung ◽  
...  
Keyword(s):  
Cellular Uptake ◽  
Gold Nanorods ◽  
Aspect Ratios

scholarly journals Direct formation of gold nanorods on surfaces using polymer-immobilised gold seeds

2016 ◽  
Vol 7 ◽  
pp. 809-816 ◽  
Author(s):  
Majid K Abyaneh ◽  
Pietro Parisse ◽  
Loredana Casalis
Keyword(s):  
Gold Nanorods ◽  
Growth Control ◽  
Weight Ratio ◽  
Polymer Nanocomposite ◽  
Gold Salt ◽  
Aspect Ratios ◽  
Direct Formation ◽  
Growth Method ◽  
Direct Growth ◽  
Gold Seed

Herein, we present the formation of gold nanorods (GNRs) on novel gold–poly(methyl methacrylate) (Au–PMMA) nanocomposite substrates with unprecedented growth control through the polymer molecular weight (M w) and gold-salt-to-polymer weight ratio. For the first time, GNRs have been produced by seed-mediated direct growth on surfaces that were pre-coated with polymer-immobilised gold seeds. A Au–PMMA nanocomposite formed by UV photoreduction has been used as the gold seed. The influence of polymer M w and gold concentration on the formation of GNRs has been investigated and discussed. The polymer nanocomposite formed with a lower M w PMMA and 20 wt % gold salt provides a suitable medium for growing well-dispersed GNRs. In this sample, the average dimension of produced GNRs is 200 nm in length with aspect ratios up to 10 and a distribution of GNRs to nanoparticles of nearly 22%. Suitable characterization techniques such as AFM and SEM have been used to support concept of the proposed growth method.

Membrane Microdomain Structures of Liposomes and Their Contribution to the Cellular Uptake Efficiency into HeLa Cells

2016 ◽  
Vol 13 (2) ◽  
pp. 369-378 ◽  
Author(s):  
Yoshinori Onuki ◽  
Yasuko Obata ◽  
Kumi Kawano ◽  
Hiromu Sano ◽  
Reina Matsumoto ◽  
...  
Keyword(s):  
Cellular Uptake ◽  
Hela Cells ◽  
Uptake Efficiency ◽  
Membrane Microdomain ◽  
Cellular Uptake Efficiency

Surface modification of gold nanorods using layer-by-layer technique for cellular uptake

2007 ◽  
Vol 10 (1) ◽  
pp. 221-228 ◽  
Author(s):  
Hironobu Takahashi ◽  
Takuro Niidome ◽  
Takahito Kawano ◽  
Sunao Yamada ◽  
Yasuro Niidome
Keyword(s):  
Surface Modification ◽  
Cellular Uptake ◽  
Gold Nanorods ◽  
Layer By Layer ◽  
Layer Technique ◽  
Layer By Layer Technique

scholarly journals Two-step synthesis of high aspect ratio gold nanorods

2006 ◽  
Vol 4 (1) ◽  
pp. 160-165 ◽  
Author(s):  
Tom Mortier ◽  
André Persoons ◽  
Thierry Verbiest
Keyword(s):  
Aspect Ratio ◽  
Gold Nanorods ◽  
High Aspect Ratio ◽  
Cetyltrimethylammonium Bromide ◽  
Surfactant Concentration ◽  
Synthetic Method ◽  
Aspect Ratios ◽  
Seed Mediated

AbstractWe describe a very simple, two-step synthetic method to prepare gold nanorods with extremely high aspect ratios (> 20) and average lengths of more than 1000 nm. The method is based on a seed-mediated growth in presence of the surfactant cetyltrimethylammonium bromide. The length and aspect ratios of the nanorods can be manipulated by varying the surfactant concentration.

scholarly journals Coupled elasticity–diffusion model for the effects of cytoskeleton deformation on cellular uptake of cylindrical nanoparticles

2015 ◽  
Vol 12 (102) ◽  
pp. 20141023 ◽  
Author(s):  
Jizeng Wang ◽  
Long Li
Keyword(s):  
Diffusion Model ◽  
Cellular Uptake ◽  
Configurational Entropy ◽  
Host Cells ◽  
Target Cells ◽  
Animal Cells ◽  
Dynamic Simulations ◽  
Aspect Ratios ◽  
Dynamics Simulations ◽  
Membrane Deformations

Molecular dynamic simulations and experiments have recently demonstrated how cylindrical nanoparticles (CNPs) with large aspect ratios penetrate animal cells and inevitably deform cytoskeletons. Thus, a coupled elasticity–diffusion model was adopted to elucidate this interesting biological phenomenon by considering the effects of elastic deformations of cytoskeleton and membrane, ligand–receptor binding and receptor diffusion. The mechanism by which the binding energy drives the CNPs with different orientations to enter host cells was explored. This mechanism involved overcoming the resistance caused by cytoskeleton and membrane deformations and the change in configurational entropy of the ligand–receptor bonds and free receptors. Results showed that deformation of the cytoskeleton significantly influenced the engulfing process by effectively slowing down and even hindering the entry of the CNPs. Additionally, the engulfing depth was determined quantitatively. CNPs preferred or tended to vertically attack target cells until they were stuck in the cytoskeleton as implied by the speed of vertically oriented CNPs that showed much faster initial engulfing speeds than horizontally oriented CNPs. These results elucidated the most recent molecular dynamics simulations and experimental observations on the cellular uptake of carbon nanotubes and phagocytosis of filamentous Escherichia coli bacteria. The most efficient engulfment showed the stiffness-dependent optimal radius of the CNPs. Cytoskeleton stiffness exhibited more significant influence on the optimal sizes of the vertical uptake than the horizontal uptake.

Synthesis of Gold Nanoparticles Coated with pH-Responsive Polymers and Evaluation of the Cellular Uptake

2012 ◽  
Vol 1416 ◽  
Author(s):  
Takeo Ito ◽  
Eriko Kusaka ◽  
Yu Isobe ◽  
Sei-ichi Nishimoto
Keyword(s):  
Cellular Uptake ◽  
Gold Nanorods ◽  
Near Infrared ◽  
A549 Cells ◽  
Endocytic Pathway ◽  
Human Lung Cancer ◽  
Ph Responsive ◽  
Absorption Bands ◽  
Responsive Polymers ◽  
Ph Responsive Polymers

ABSTRACTGold nanorods (AuNRs) show surface plasmon absorption bands in the near-infrared region. This characteristic property has stimulated utilization of gold nanorods as novel nanoprobes for noninvasive bioimaging, such as photoacoustic tomography. Herein, we discuss the synthesis of a series of gold nanorods coated with pH-responsive polymers to investigate the effect of the surface structure and zeta potential of nanoparticles on cellular uptake via a surface charge-mediated endocytic pathway. The surface of the gold nanorods was modified with polyethylene glycol (PEG@AuNR) and tertiary amine derivatives, specifically, diethylaminoethyl ester (1@AuNRs), its amide analog (2@AuNRs), and dimethylaminoethyl ester (3@AuNRs). It was found that the pH-sensitivity of1@AuNRs was relatively high and the surface was positively charged at lower pH. In contrast, the tertiary amino group of1@AuNRs was deprotonated to form an electrostatically neutral surface at higher pH. The pH-responsive gold nanorods were incubated with A549 cells (human lung cancer cells) to quantify the amount of cellular uptake using inductively coupled plasma mass spectrometry. The results indicate that1@AuNRs can be taken up efficiently in the cells, and thereafter, slowly flow out of the cells. Interestingly, only small amounts of the amide analog (2@AuNRs) were taken into the cells, suggesting minor structural changes may affect the interaction between the cell surface and AuNRs. This study highlights a potential application of pH-sensitive nanorods as a probe for bioimaging the acidic environment of tumors.

The influence of cellular uptake on gold nanorods photostability and photoacoustic conversion efficiency

2015 ◽  
Author(s):  
Lucia Cavigli ◽  
Fulvio Ratto ◽  
Francesca Tatini ◽  
Paolo Matteini ◽  
Alberto Cini ◽  
...  
Keyword(s):  
Cellular Uptake ◽  
Conversion Efficiency ◽  
Gold Nanorods

Preparation and In Vitro Cellular Uptake Assessment of Multifunctional Rubik-Like Magnetic Nano-Assemblies

2019 ◽  
Vol 19 (6) ◽  
pp. 3301-3309
Author(s):  
Xiawen Zheng ◽  
Yuejian Chen ◽  
Zhiming Wang ◽  
Lina Song ◽  
Yu Zhang ◽  
...  
Keyword(s):  
Oleic Acid ◽  
Folic Acid ◽  
Cellular Uptake ◽  
Hela Cells ◽  
Drug Loading ◽  
Superparamagnetic Iron Oxide ◽  
Drug Carriers ◽  
High Drug ◽  
High Drug Loading

Through self-assembly of nanoparticles into high-order and stable structures of cubic clusters, high drug-loading rubik-like magnetic nano-assemblies (MNAs), possessing folic acid targeting and strong magnetism-enhanced cellular uptake capabilities, were built. In this study, the core of the cubic drug assemblies consisted of four monodisperse superparamagnetic iron oxide nanoparticles coated with layers of oleic acid (Fe3O4@OA), simultaneously encapsulating fluorescein, and Paclitaxol (Flu-MNAs and PTX-MNAs) for imaging and therapeutic applications. To enable preferential tumor cellular uptake by the nanocarriers, the outermost layer of Fe3O4 was functionalized with the new dual-oleic acid-polyethylene glycol-folic acid polymer (FA-PEG-Lys-OA2) as a “shell.” The drug carriers exhibited excellent stability and biocompatibility, and showed high drug loading and excellent magnetic response In Vitro. Furthermore, preliminary evaluations of the drug carriers with Hela cells showed effective cellular targeting capability. In addition, the cubic assemblies enhanced anticancer efficiency for Hela cells compared to bare drugs. Especially, the applied external magnetic field further improved the uptake of the vectors, and thereby enhanced the inhibitory effect. In brief, all these results suggested that cubic assemblies could serve as potential strategies for targeted anticancer therapies.

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