Highly Luminescent Thermoresponsive Green Emitting Gold Nanoclusters for Intracellular Nanothermometry and Cellular Imaging: A Dual Function Optical Probe

2019 ◽  
Vol 2 (5) ◽  
pp. 2078-2091 ◽  
Author(s):  
Sangita Kundu ◽  
Devdeep Mukherjee ◽  
Tapas Kumar Maiti ◽  
Nilmoni Sarkar
Keyword(s):  
Gold Nanoclusters ◽  
Optical Probe ◽  
Cellular Imaging ◽  
Dual Function

A New Strategy to Construct Gold Nanoclusters-Based Optical Probe Using Luminescence Resonance Energy Transfer

2021 ◽  
Author(s):  
Shuyang Zhai ◽  
Wei Hu ◽  
Chen Fan ◽  
Wenqi Feng ◽  
Zhi-hong Liu
Keyword(s):  
Energy Transfer ◽  
Gold Nanoclusters ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Optical Probe ◽  
Luminescent Materials ◽  
Metal Nanoclusters ◽  
New Strategy ◽  
Photoluminescence Efficiency

Monolayer-protected metal nanoclusters (MPCs) are emerging as intriguing luminescent materials, but the construction of MPCs-based optical probe is still scarce because of both the limited photoluminescence efficiency of MPCs and...

scholarly journals Synthesis of Exosome-Based Fluorescent Gold Nanoclusters for Cellular Imaging Applications

2021 ◽  
Vol 22 (9) ◽  
pp. 4433
Author(s):  
Eun Sung Lee ◽  
Byung Seok Cha ◽  
Seokjoon Kim ◽  
Ki Soo Park
Keyword(s):  
Photoelectron Spectroscopy ◽  
Low Cost ◽  
Stokes Shift ◽  
Gold Nanoclusters ◽  
Human Colon ◽  
Cellular Imaging ◽  
Metal Nanoclusters ◽  
Human Colon Cancer ◽  
X Ray ◽  
High Concentrations

In recent years, fluorescent metal nanoclusters have been used to develop bioimaging and sensing technology. Notably, protein-templated fluorescent gold nanoclusters (AuNCs) are attracting interest due to their excellent fluorescence properties and biocompatibility. Herein, we used an exosome template to synthesize AuNCs in an eco-friendly manner that required neither harsh conditions nor toxic chemicals. Specifically, we used a neutral (pH 7) and alkaline (pH 11.5) pH to synthesize two different exosome-based AuNCs (exo-AuNCs) with independent blue and red emission. Using field-emission scanning electron microscopy, energy dispersive X-ray microanalysis, nanoparticle tracking analysis, and X-ray photoelectron spectroscopy, we demonstrated that AuNCs were successfully formed in the exosomes. Red-emitting exo-AuNCs were found to have a larger Stokes shift and a stronger fluorescence intensity than the blue-emitting exo-AuNCs. Both exo-AuNCs were compatible with MCF-7 (human breast cancer), HeLa (human cervical cancer), and HT29 (human colon cancer) cells, although blue-emitting exo-AuNCs were cytotoxic at high concentrations (≥5 mg/mL). Red-emitting exo-AuNCs successfully stained the nucleus and were compatible with membrane-staining dyes. This is the first study to use exosomes to synthesize fluorescent nanomaterials for cellular imaging applications. As exosomes are naturally produced via secretion from almost all types of cell, the proposed method could serve as a strategy for low-cost production of versatile nanomaterials.

Surface Ligand-Controlled Wavelength-Tunable Luminescence of Gold Nanoclusters: Cellular Imaging and Smart Fluorescent Probes for Amyloid Detection

2020 ◽  
Vol 3 (7) ◽  
pp. 4282-4293 ◽  
Author(s):  
Sangita Kundu ◽  
Biswajoy Ghosh ◽  
Sourav Nandi ◽  
Meghna Ghosh ◽  
Arghajit Pyne ◽  
...  
Keyword(s):  
Fluorescent Probes ◽  
Gold Nanoclusters ◽  
Cellular Imaging ◽  
Wavelength Tunable ◽  
Surface Ligand

Facile preparation of water-soluble fluorescent gold nanoclusters for cellular imaging applications

Nanoscale ◽  
2011 ◽  
Vol 3 (5) ◽  
pp. 2009 ◽  
Author(s):  
Li Shang ◽  
René M. Dörlich ◽  
Stefan Brandholt ◽  
Reinhard Schneider ◽  
Vanessa Trouillet ◽  
...  
Keyword(s):  
Gold Nanoclusters ◽  
Cellular Imaging ◽  
Water Soluble ◽  
Facile Preparation

Facile synthesis of fluorescent gold nanoclusters and their application in cellular imaging

2012 ◽  
Author(s):  
Li Shang ◽  
René M. Dörlich ◽  
Stefan Brandholt ◽  
Naghmeh Azadfar ◽  
Gerd Ulrich Nienhaus
Keyword(s):  
Gold Nanoclusters ◽  
Cellular Imaging ◽  
Facile Synthesis

Application of Biomimetic Nanomaterials in Biological Detection and the Intelligent Recognition Method of Nanoparticle Images

2021 ◽  
Vol 16 (1) ◽  
pp. 23-30
Author(s):  
Ying He
Keyword(s):  
Image Segmentation ◽  
Fluorescent Probe ◽  
Gold Nanoclusters ◽  
Silver Ions ◽  
Silver Nanoclusters ◽  
Dual Function ◽  
Mercury Ions ◽  
Watershed Transformation ◽  
Gold Silver ◽  
Biomimetic Nanomaterials

New nanomaterials (metal nanoclusters, graphene, etc.) are favored by researchers due to their unique properties and are widely used in biomedical detection. The excellent fluorescence characteristics of gold nanoclusters are utilized to develop a fast and highly sensitive bionic nanomaterial with non-label and dual functions, which can detect silver ions and mercury ions and study the particularity of TEM nanoparticle images. The particle segmentation of TEM nanoparticle images is studied to compare the traditional watershed algorithm and watershed transformation algorithm. The experiment results show that silver ions can enhance the fluorescence of gold nanoclusters to form gold-silver nanoclusters with strong yellow fluorescence, and mercury ions can quickly weaken the fluorescence of gold-silver nanoclusters. Based on the biomimetic nanomaterials, a dual-function fluorescent probe is designed to detect silver ions and mercury ions in lake with detection accuracy of 8 nM and 33 nM respectively; the sensing excitation of the fluorescent probe is further analyzed. Because the metal-enhanced fluorescence (MEF) effect enables the silver element and Au nanoparticles to form fluorescence-enhancing effect, the high metalphilic interaction between mercury ions and silver ions quenches the fluorescence effect of gold nanocluster; the rapid watershed transformation/region fusion method can achieve better particle image segmentation combined with the image segmentation algorithms of different TEM nanoparticles, which can be better applied to the characterization analysis of the preparation of gold nanomaterials.

PEGylated Luminescent Gold Nanoclusters: Synthesis, Characterization, Bioconjugation, and Application to One- and Two-Photon Cellular Imaging

2013 ◽  
Vol 30 (5) ◽  
pp. 453-466 ◽  
Author(s):  
Eunkeu Oh ◽  
Fredrik K. Fatemi ◽  
Marc Currie ◽  
James B. Delehanty ◽  
Thomas Pons ◽  
...  
Keyword(s):  
Gold Nanoclusters ◽  
Cellular Imaging ◽  
Two Photon ◽  
Luminescent Gold Nanoclusters
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