PEGylated Luminescent Gold Nanoclusters: Synthesis, Characterization, Bioconjugation, and Application to One- and Two-Photon Cellular Imaging

2013 ◽  
Vol 30 (5) ◽  
pp. 453-466 ◽  
Author(s):  
Eunkeu Oh ◽  
Fredrik K. Fatemi ◽  
Marc Currie ◽  
James B. Delehanty ◽  
Thomas Pons ◽  
...  
Keyword(s):  
Gold Nanoclusters ◽  
Cellular Imaging ◽  
Two Photon ◽  
Luminescent Gold Nanoclusters

scholarly journals Synthesis of Exosome-Based Fluorescent Gold Nanoclusters for Cellular Imaging Applications

2021 ◽  
Vol 22 (9) ◽  
pp. 4433
Author(s):  
Eun Sung Lee ◽  
Byung Seok Cha ◽  
Seokjoon Kim ◽  
Ki Soo Park
Keyword(s):  
Photoelectron Spectroscopy ◽  
Low Cost ◽  
Stokes Shift ◽  
Gold Nanoclusters ◽  
Human Colon ◽  
Cellular Imaging ◽  
Metal Nanoclusters ◽  
Human Colon Cancer ◽  
X Ray ◽  
High Concentrations

In recent years, fluorescent metal nanoclusters have been used to develop bioimaging and sensing technology. Notably, protein-templated fluorescent gold nanoclusters (AuNCs) are attracting interest due to their excellent fluorescence properties and biocompatibility. Herein, we used an exosome template to synthesize AuNCs in an eco-friendly manner that required neither harsh conditions nor toxic chemicals. Specifically, we used a neutral (pH 7) and alkaline (pH 11.5) pH to synthesize two different exosome-based AuNCs (exo-AuNCs) with independent blue and red emission. Using field-emission scanning electron microscopy, energy dispersive X-ray microanalysis, nanoparticle tracking analysis, and X-ray photoelectron spectroscopy, we demonstrated that AuNCs were successfully formed in the exosomes. Red-emitting exo-AuNCs were found to have a larger Stokes shift and a stronger fluorescence intensity than the blue-emitting exo-AuNCs. Both exo-AuNCs were compatible with MCF-7 (human breast cancer), HeLa (human cervical cancer), and HT29 (human colon cancer) cells, although blue-emitting exo-AuNCs were cytotoxic at high concentrations (≥5 mg/mL). Red-emitting exo-AuNCs successfully stained the nucleus and were compatible with membrane-staining dyes. This is the first study to use exosomes to synthesize fluorescent nanomaterials for cellular imaging applications. As exosomes are naturally produced via secretion from almost all types of cell, the proposed method could serve as a strategy for low-cost production of versatile nanomaterials.

Microwave-assisted rapid synthesis of luminescent gold nanoclusters for sensing Hg2+ in living cells using fluorescence imaging

Nanoscale ◽  
2012 ◽  
Vol 4 (14) ◽  
pp. 4155 ◽  
Author(s):  
Li Shang ◽  
Linxiao Yang ◽  
Florian Stockmar ◽  
Radian Popescu ◽  
Vanessa Trouillet ◽  
...  
Keyword(s):  
Fluorescence Imaging ◽  
Gold Nanoclusters ◽  
Living Cells ◽  
Rapid Synthesis ◽  
Microwave Assisted ◽  
Luminescent Gold Nanoclusters

scholarly journals Two-Photon Time-Gated In Vivo Imaging of Dihydrolipoic-Acid-Decorated Gold Nanoclusters

Materials ◽  
2021 ◽  
Vol 14 (24) ◽  
pp. 7744
Author(s):  
Ye Tian ◽  
Ming Wei ◽  
Lijun Wang ◽  
Yuankai Hong ◽  
Dan Luo ◽  
...  
Keyword(s):  
In Vivo Imaging ◽  
Gold Nanoclusters ◽  
Photon Absorption ◽  
Gold Nanocluster ◽  
Time Resolved ◽  
Dihydrolipoic Acid ◽  
Two Photon Absorption ◽  
Two Photon ◽  
Time Resolved Imaging

Due to the unique advantages of two-photon technology and time-resolved imaging technology in the biomedical field, attention has been paid to them. Gold clusters possess excellent physicochemical properties and low biotoxicity, which make them greatly advantageous in biological imaging, especially for in vivo animal imaging. A gold nanocluster was coupled with dihydrolipoic acid to obtain a functionalized nanoprobe; the material displayed significant features, including a large two-photon absorption cross-section (up to 1.59 × 105 GM) and prolonged fluorescence lifetime (>300 ns). The two-photon and time-resolution techniques were used to perform cell imaging and in vivo imaging.

Synthesis, Two-Photon Absorption, and Cellular Imaging of Two Curcumin Derivatives

2019 ◽  
Vol 89 (12) ◽  
pp. 2577-2583
Author(s):  
W. Zhang ◽  
S. S. Zhou ◽  
G. F. Si ◽  
J. F. Wang ◽  
G. Y. Xu
Keyword(s):  
Cellular Imaging ◽  
Photon Absorption ◽  
Two Photon Absorption ◽  
Two Photon ◽  
Curcumin Derivatives

Dual targeting luminescent gold nanoclusters for tumor imaging and deep tissue therapy

Biomaterials ◽  
2016 ◽  
Vol 100 ◽  
pp. 1-16 ◽  
Author(s):  
Dan Chen ◽  
Bowen Li ◽  
Songhua Cai ◽  
Peng Wang ◽  
Shuwen Peng ◽  
...  
Keyword(s):  
Tumor Imaging ◽  
Gold Nanoclusters ◽  
Deep Tissue ◽  
Dual Targeting ◽  
Tissue Therapy ◽  
Luminescent Gold Nanoclusters

Efficient two-photon luminescence for cellular imaging using biocompatible nitrogen-doped graphene quantum dots conjugated with polymers

Nanoscale ◽  
2018 ◽  
Vol 10 (1) ◽  
pp. 109-117 ◽  
Author(s):  
Ping-Ching Wu ◽  
Jiu-Yao Wang ◽  
Wen-Lung Wang ◽  
Chia-Yuan Chang ◽  
Chia-Hung Huang ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Contrast Agent ◽  
Graphene Quantum Dots ◽  
Cellular Imaging ◽  
Deep Tissue ◽  
Nitrogen Doped ◽  
Two Photon ◽  
Nitrogen Doped Graphene ◽  
Doped Graphene

N-doped GQDs conjugated with polymers demonstrate impressive two-photon properties and stability, and can be used as a contrast agent in deep tissue bioimaging.

scholarly journals Reaction-based two-photon probes for in vitro analysis and cellular imaging of monoamine oxidase activity

2012 ◽  
Vol 48 (54) ◽  
pp. 6833 ◽  
Author(s):  
Dokyoung Kim ◽  
Sunderraman Sambasivan ◽  
Hyoseok Nam ◽  
Ki Hean Kim ◽  
Jin Yong Kim ◽  
...  
Keyword(s):  
Monoamine Oxidase ◽  
Oxidase Activity ◽  
Cellular Imaging ◽  
Monoamine Oxidase Activity ◽  
Two Photon ◽  
Vitro Analysis ◽  
In Vitro Analysis
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