Injectable Cell- and Growth Factor-Free Poly(4-hydroxybutyrate) (P4HB) Microspheres with Open Porous Structures and Great Efficiency of Promoting Bone Regeneration

2021 ◽  
Author(s):  
Shuaishuai Yuan ◽  
Yong Shen ◽  
Zhibo Li
Keyword(s):  
Growth Factor ◽  
Bone Regeneration ◽  
Porous Structures ◽  
Great Efficiency

Triple Growth Factor Delivery Promotes Functional Bone Regeneration Following Composite Musculoskeletal Trauma

2021 ◽  
Author(s):  
Ramesh Subbiah ◽  
Marissa A. Ruehle ◽  
Brett S. Klosterhoff ◽  
Angela S.P. Lin ◽  
Marian H. Hettiaratchi ◽  
...  
Keyword(s):  
Growth Factor ◽  
Bone Regeneration ◽  
Growth Factor Delivery ◽  
Musculoskeletal Trauma

A Review of Platelet Derived Growth Factor Playing Pivotal Role in Bone Regeneration

2014 ◽  
Vol 40 (3) ◽  
pp. 330-340 ◽  
Author(s):  
Prasun Shah ◽  
Louise Keppler ◽  
James Rutkowski
Keyword(s):  
Growth Factor ◽  
Bone Regeneration ◽  
Bone Grafting ◽  
Bone Growth ◽  
Platelet Derived Growth Factor ◽  
Future Application ◽  
Second Phase ◽  
Bony Defect ◽  
Wound Site ◽  
Functioning Capillaries

This article is focused on the literature review and study of recent advances in the field of bone grafting, which involves platelet-derived growth factor (PDGF) as one of the facilitating factors in bone regeneration. This article includes a description of the mechanism of PDGF for use in surgeries where bone grafting is required, which promotes future application of PDGF for faster bone regeneration or inhibition of bone growth if required as in osteosarcoma. The important specific activities of PDGF include mitogenesis (increase in the cell populations of healing cells), angiogenesis (endothelial mitoses into functioning capillaries), and macrophage activation (debridement of the wound site and a second phase source of growth factors for continued repair and bone regeneration). Thus PDGF can be utilized in wound with bone defect to conceal the wound with repair of bony defect.

scholarly journals A Growth Factor-Free Co-Culture System of Osteoblasts and Peripheral Blood Mononuclear Cells for the Evaluation of the Osteogenesis Potential of Melt-Electrowritten Polycaprolactone Scaffolds

2019 ◽  
Vol 20 (5) ◽  
pp. 1068 ◽  
Author(s):  
Andreas Hammerl ◽  
Carlos Diaz Cano ◽  
Elena De-Juan-Pardo ◽  
Martijn van Griensven ◽  
Patrina Poh
Keyword(s):  
Growth Factor ◽  
Bone Regeneration ◽  
Peripheral Blood Mononuclear Cells ◽  
Peripheral Blood ◽  
Culture System ◽  
Mononuclear Cells ◽  
Regeneration Potential ◽  
Peripheral Blood Mononuclear ◽  
Blood Mononuclear Cells

Scaffolds made of biodegradable biomaterials are widely used to guide bone regeneration. Commonly, in vitro assessment of scaffolds’ osteogenesis potential has been performed predominantly in monoculture settings. Hence, this study evaluated the potential of an unstimulated, growth factor-free co-culture system comprised of osteoblasts (OB) and peripheral blood mononuclear cells (PBMC) over monoculture of OB as an in vitro platform for screening of bone regeneration potential of scaffolds. Particularly, this study focuses on the osteogenic differentiation and mineralized matrix formation aspects of cells. The study was performed using scaffolds fabricated by means of a melt electrowriting (MEW) technique made of medical-grade polycaprolactone (PCL), with or without a surface coating of calcium phosphate (CaP). Qualitative results, i.e., cell morphology by fluorescence imaging and matrix mineralization by von Kossa staining, indicated the differences in cell behaviours in response to scaffolds’ biomaterial. However, no obvious differences were noted between OB and OB+PBMC groups. Hence, quantitative investigation, i.e., alkaline phosphatase (ALP), tartrate-resistant acid phosphatase (TRAP) activities, and gene expression were quantitatively evaluated by reverse transcription-polymerase chain reaction (RT-qPCR), were evaluated only of PCL/CaP scaffolds cultured with OB+PBMC, while PCL/CaP scaffolds cultured with OB or PBMC acted as a control. Although this study showed no differences in terms of osteogenic differentiation and ECM mineralization, preliminary qualitative results indicate an obvious difference in the cell/non-mineralized ECM density between scaffolds cultured with OB or OB+PBMC that could be worth further investigation. Collectively, the unstimulated, growth factor-free co-culture (OB+PBMC) system presented in this study could be beneficial for the pre-screening of scaffolds’ in vitro bone regeneration potential prior to validation in vivo.

scholarly journals Clinical application of injectable growth factor for bone regeneration: a systematic review

2019 ◽  
Vol 39 (1) ◽  
Author(s):  
Yutaka Kuroda ◽  
Toshiyuki Kawai ◽  
Koji Goto ◽  
Shuichi Matsuda
Keyword(s):  
Growth Factors ◽  
Growth Factor ◽  
Femoral Head ◽  
Bone Regeneration ◽  
Clinical Application ◽  
Present Report ◽  
Basic Research ◽  
Limb Ischemia ◽  
Efficacy And Safety ◽  
Gelatin Hydrogel

Abstract Bone regeneration has been the ultimate goal in the field of bone and joint medicine and has been evaluated through various basic research studies to date. Translational research of regenerative medicine has focused on three primary approaches, which are expected to increase in popularity: cell therapy, proteins, and artificial materials. Among these, the local injection of a gelatin hydrogel impregnated with the protein fibroblast growth factor (FGF)-2 is a biomaterial technique that has been developed in Japan. We have previously reported the efficacy of gelatin hydrogel containing injectable FGF-2 for the regenerative treatment of osteonecrosis of the femoral head. Injectable growth factors will probably be developed in the future and gain popularity as a medical approach in various fields as well as orthopedics. Several clinical trials have already been conducted and have focused on this technique, reporting its efficacy and safety. To date, reports of the clinical application of FGF-2 in revascularization for critical limb ischemia, treatment of periodontal disease, early bone union for lower limb fracture and knee osteotomy, and bone regeneration for osteonecrosis of the femoral head have been based on basic research conducted in Japan. In the present report, we present an extensive review of clinical applications using injectable growth factors and discuss the associated efficacy and safety of their administration.

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