The effect of basic fibroblast growth factor on bone regeneration when released from a novelin situsetting tricalcium phosphate cement

2004 ◽  
Vol 69A (4) ◽  
pp. 680-685 ◽  
Author(s):  
Christopher Niedhart ◽  
Uwe Maus ◽  
Oliver Miltner ◽  
Hans G. Gräber ◽  
Fritz U. Niethard ◽  
...  
Keyword(s):  
Growth Factor ◽  
Bone Regeneration ◽  
Fibroblast Growth Factor ◽  
Basic Fibroblast Growth Factor ◽  
Tricalcium Phosphate ◽  
Fibroblast Growth

Potential efficacy of basic fibroblast growth factor incorporated in biodegradable hydrogels for skull bone regeneration

1997 ◽  
Vol 86 (5) ◽  
pp. 871-875 ◽  
Author(s):  
Keisuke Yamada ◽  
Yasuhiko Tabata ◽  
Kazuo Yamamoto ◽  
Susumu Miyamoto ◽  
Izumi Nagata ◽  
...  
Keyword(s):  
Aqueous Solution ◽  
Growth Factor ◽  
Bone Regeneration ◽  
Fibroblast Growth Factor ◽  
Basic Fibroblast Growth Factor ◽  
Fibrous Tissue ◽  
Rabbit Model ◽  
Fibroblast Growth ◽  
Skull Defect ◽  
Content Type

✓ Biodegradable gelatin hydrogels incorporating basic fibroblast growth factor (bFGF) were evaluated for their efficacy in bone regeneration using a rabbit model. Hydrogels with water contents of 85% and 98% were prepared using chemical crosslinking of gelatin with an isoelectric point of 4.9 in aqueous solution and, after freeze drying, were impregnated with an aqueous solution of bFGF to obtain bFGF-incorporated gelatin hydrogels. When they were implanted into bone defects measuring 6 mm in diameter in rabbit skulls (six animals/group), complete closure of the defect was observed at 12 weeks after implantation, regardless of the water content of the hydrogels. In contrast, bFGF did not enhance bone regeneration when applied to the skull defect in solution with phosphate-buffered saline (PBS). Also, gelatin hydrogels lacking bFGF were not effective in inducing bone formation, with fibrous tissue growing into the defect instead, similar to the skull defect seen in control rabbits treated with PBS. This indicates that the presence of hydrogels did not interfere with bone regeneration at the skull defect, probably because of their disappearance during biodegradation. It is concluded that the gelatin hydrogel is a promising matrix for effective induction of biological activity of bFGF for bone regeneration in skull and sinus defects.

Skull bone regeneration in primates in response to basic fibroblast growth factor

1999 ◽  
Vol 91 (5) ◽  
pp. 851-856 ◽  
Author(s):  
Yasuhiko Tabata ◽  
Keisuke Yamada ◽  
Liu Hong ◽  
Susumu Miyamoto ◽  
Nobuo Hashimoto ◽  
...  
Keyword(s):  
Growth Factor ◽  
Bone Regeneration ◽  
Fibroblast Growth Factor ◽  
Basic Fibroblast Growth Factor ◽  
Fibroblast Growth ◽  
Skull Defect ◽  
Skull Bone ◽  
Content Type ◽  
X Ray ◽  
Fine Print

Object. The feasibility of using a biodegradable hydrogel incorporating basic fibroblast growth factor (bFGF) to induce bone regeneration at the site of a skull defect in monkeys was investigated.Methods. Basic fibroblast growth factor was incorporated into a bioabsorbable hydrogel, which was prepared through glutaraldehyde crosslinking of gelatin. Following treatment of monkey skull defects measuring 6 mm in diameter (six defects/experimental group) with gelatin hydrogel incorporating bFGF, skull bone regeneration was evaluated using soft x-ray studies, dual x-ray absorptometry, and histological examinations.The water content of the hydrogels varied according to the glutaraldehyde concentration in the hydrogel preparation. Gelatin hydrogels incorporating 100 µg of bFGF significantly promoted bone regeneration and the skull defect was completely closed 21 weeks after implantation. This is in marked contrast with the effect of the same dose of bFGF in solution form. Bone mineral density (BMD) measured at the sites of skull defect was enhanced by the bFGF-incorporating hydrogels. The BMD enhancement was more prominent at lower water contents of hydrogel. Empty gelatin hydrogels neither induced nor interfered with skull bone regeneration.Conclusions. The findings of this study indicate that bFGF coupled with bioabsorbable hydrogel is a very promising tool to assist in the regrowth of bone at the site of a skull defect, which clinically has been recognized as almost impossible.

Enhanced bone regeneration with sequential delivery of basic fibroblast growth factor and sonic hedgehog

Injury ◽  
2011 ◽  
Vol 42 (8) ◽  
pp. 796-802 ◽  
Author(s):  
Ke Song ◽  
Nian-Jing Rao ◽  
Mei-Ling Chen ◽  
Zheng-Jiang Huang ◽  
Ying-Guang Cao
Keyword(s):  
Growth Factor ◽  
Bone Regeneration ◽  
Fibroblast Growth Factor ◽  
Sonic Hedgehog ◽  
Basic Fibroblast Growth Factor ◽  
Fibroblast Growth
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