Hydrophilic Interaction Chromatography-Based High-Throughput Sample Preparation Method for N-Glycan Analysis from Total Human Plasma Glycoproteins

2008 ◽  
Vol 80 (15) ◽  
pp. 6119-6126 ◽  
Author(s):  
L. Renee Ruhaak ◽  
Carolin Huhn ◽  
Willem-Jan Waterreus ◽  
Arjen R. de Boer ◽  
Christian Neusüss ◽  
...  
Keyword(s):  
Sample Preparation ◽  
Human Plasma ◽  
High Throughput ◽  
Preparation Method ◽  
Hydrophilic Interaction Chromatography ◽  
Hydrophilic Interaction ◽  
Sample Preparation Method ◽  
Glycan Analysis

scholarly journals Sample preparation method to improve the efficiency of high-throughput single-molecule force spectroscopy

2019 ◽  
Vol 5 (4) ◽  
pp. 176-183 ◽  
Author(s):  
Lei Jin ◽  
Li Kou ◽  
Yanan Zeng ◽  
Chunguang Hu ◽  
Xiaodong Hu
Keyword(s):  
Sample Preparation ◽  
High Throughput ◽  
Single Molecule ◽  
Preparation Method ◽  
Force Spectroscopy ◽  
Experimental Result ◽  
Sample Preparation Method ◽  
Dna Stretching ◽  
Single Molecule Force Spectroscopy ◽  
Preparation Methods

Abstract Inefficient sample preparation methods hinder the performance of high-throughput single-molecule force spectroscopy (H-SMFS) for viscous damping among reactants and unstable linkage. Here, we demonstrated a sample preparation method for H-SMFS systems to achieve a higher ratio of effective target molecules per sample cell by gas-phase silanization and reactant hydrophobization. Digital holographic centrifugal force microscopy (DH-CFM) was used to verify its performance. The experimental result indicated that the DNA stretching success ratio was improved from 0.89% to 13.5%. This enhanced efficiency preparation method has potential application for force-based DNA stretching experiments and other modifying procedures.

scholarly journals Improved sample preparation method for glycan analysis of glycoproteins by CE-LIF and CE-MS

2010 ◽  
Vol 31 (8) ◽  
pp. 1389-1395 ◽  
Author(s):  
Zoltan Szabo ◽  
András Guttman ◽  
Tomas Rejtar ◽  
Barry L. Karger
Keyword(s):  
Sample Preparation ◽  
Preparation Method ◽  
Sample Preparation Method ◽  
Glycan Analysis

A high-throughput sample preparation method for cellular proteomics using 96-well filter plates

PROTEOMICS ◽  
2013 ◽  
Vol 13 (20) ◽  
pp. 2980-2983 ◽  
Author(s):  
Linda Switzar ◽  
Jordy van Angeren ◽  
Martijn Pinkse ◽  
Jeroen Kool ◽  
Wilfried M. A. Niessen
Keyword(s):  
Sample Preparation ◽  
High Throughput ◽  
Preparation Method ◽  
Sample Preparation Method

Improved sample preparation method for glycan analysis of glycoproteins by CE-LIF and CE-MS

2010 ◽  
pp. NA-NA
Author(s):  
Zoltan Szabo ◽  
András Guttman ◽  
Tomas Rejtar ◽  
Barry L. Karger
Keyword(s):  
Sample Preparation ◽  
Preparation Method ◽  
Sample Preparation Method ◽  
Glycan Analysis

An automated high-throughput sample preparation method using double-filtration for serum metabolite LC-MS analysis

2019 ◽  
Vol 11 (31) ◽  
pp. 4060-4065 ◽  
Author(s):  
Minjoong Joo ◽  
Jong-Moon Park ◽  
Van-An Duong ◽  
Dami Kwon ◽  
Jongho Jeon ◽  
...  
Keyword(s):  
Sample Preparation ◽  
High Throughput ◽  
Preparation Method ◽  
Sample Preparation Method ◽  
Ms Analysis ◽  
System P ◽  
Filtration System ◽  
Automated Sample Preparation ◽  
Double Filtration

A novel, automated sample preparation method for serum metabolite LC-MS analysis was developed using a double-filtration system.

scholarly journals Development of a 96-well plate sample preparation method for integrated N- and O-glycomics using porous graphitized carbon liquid chromatography-mass spectrometry

2020 ◽  
Vol 16 (4) ◽  
pp. 355-363 ◽  
Author(s):  
Tao Zhang ◽  
Katarina Madunić ◽  
Stephanie Holst ◽  
Jing Zhang ◽  
Chunsheng Jin ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Sample Preparation ◽  
High Throughput ◽  
Preparation Method ◽  
Liquid Chromatography Mass Spectrometry ◽  
Sample Preparation Method ◽  
Porous Graphitized Carbon ◽  
Graphitized Carbon ◽  
Chromatography Mass Spectrometry

The developed workflow allows high throughput sample preparation for glycomics analysis.

scholarly journals DEVELOPMENT AND VALIDATION OF UV-VISIBLE SPECTROPHOTOMETRIC METHOD FOR ANALYSIS OF BOSENTAN IN SPIKED HUMAN PLASMA

2019 ◽  
pp. 108-110
Author(s):  
BHAVYA SRI K. ◽  
MOUNIKA C. H.
Keyword(s):  
Sample Preparation ◽  
Human Plasma ◽  
Spectrophotometric Method ◽  
Preparation Method ◽  
Analytical Techniques ◽  
Sample Preparation Method ◽  
Spiked Human Plasma ◽  
Analytical Technique ◽  
Uv Visible ◽  
Development And Validation

Objective: The aim of the present study is to develop and validate a simple, efficient, economical and accurate UV-visible spectrophotometric method for estimation of bosentan in spiked human plasma. Methods: The analyte was extracted by Liquid-liquid Extraction (LLE) procedure using acetonitrile and chloroform. Absorbance of the analyte in the extract was measured at 270 nm using ethanol as a diluent. The developed method was validated for linearity, accuracy and robustness. Results: The proposed method was found to be linear in the range of 6 to 18 mg/ml. The correlation coefficient (r2) was found to be 0.99. The results revealed that the linearity, accuracy and robustness of the developed method were within the acceptable range. Conclusion: The analytical technique presented here demonstrates shorter and easier sample preparation method, decreased analysis time and reduces the need for complicated or expensive equipment. The sample preparation method used in this study can also be further extended to higherend analytical techniques and other biological samples for quantification of bosentan.

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