scholarly journals A Simple Sample Preparation Method for Measuring Amoxicillin in Human Plasma by Hollow Fiber Centrifugal Ultrafiltration

2012 ◽  
Vol 51 (2) ◽  
pp. 181-186 ◽  
Author(s):  
Wei-Chong Dong ◽  
Zi-Li Hou ◽  
Xin-Hui Jiang ◽  
Ye Jiang
Keyword(s):  
Sample Preparation ◽  
Human Plasma ◽  
Hollow Fiber ◽  
Preparation Method ◽  
Sample Preparation Method ◽  
Simple Sample Preparation ◽  
Centrifugal Ultrafiltration

Development of a sample preparation method for acrylamide determination in cocoa via silylation

2016 ◽  
Vol 8 (29) ◽  
pp. 5874-5880 ◽  
Author(s):  
Magdalena Surma ◽  
Anna Sadowska-Rociek ◽  
Ewa Cieślik
Keyword(s):  
Sample Preparation ◽  
Preparation Method ◽  
Sample Preparation Method ◽  
Cocoa Powder ◽  
Simple Sample Preparation

This paper reports the development of a rapid and simple sample preparation method for acrylamide (AA) determination in cocoa powder through conversion to N,O-bis(trimethylsilyl)acrylamide.

A green and simple sample preparation method to determine pesticides in rice using a combination of SPME and rotating disk sorption devices

2019 ◽  
Vol 1069 ◽  
pp. 57-65 ◽  
Author(s):  
Eduarda Omena ◽  
Anderson Luiz Oenning ◽  
Josias Merib ◽  
Pablo Richter ◽  
Milton Rosero-Moreano ◽  
...  
Keyword(s):  
Sample Preparation ◽  
Preparation Method ◽  
Rotating Disk ◽  
Sample Preparation Method ◽  
Simple Sample Preparation

scholarly journals DEVELOPMENT AND VALIDATION OF UV-VISIBLE SPECTROPHOTOMETRIC METHOD FOR ANALYSIS OF BOSENTAN IN SPIKED HUMAN PLASMA

2019 ◽  
pp. 108-110
Author(s):  
BHAVYA SRI K. ◽  
MOUNIKA C. H.
Keyword(s):  
Sample Preparation ◽  
Human Plasma ◽  
Spectrophotometric Method ◽  
Preparation Method ◽  
Analytical Techniques ◽  
Sample Preparation Method ◽  
Spiked Human Plasma ◽  
Analytical Technique ◽  
Uv Visible ◽  
Development And Validation

Objective: The aim of the present study is to develop and validate a simple, efficient, economical and accurate UV-visible spectrophotometric method for estimation of bosentan in spiked human plasma. Methods: The analyte was extracted by Liquid-liquid Extraction (LLE) procedure using acetonitrile and chloroform. Absorbance of the analyte in the extract was measured at 270 nm using ethanol as a diluent. The developed method was validated for linearity, accuracy and robustness. Results: The proposed method was found to be linear in the range of 6 to 18 mg/ml. The correlation coefficient (r2) was found to be 0.99. The results revealed that the linearity, accuracy and robustness of the developed method were within the acceptable range. Conclusion: The analytical technique presented here demonstrates shorter and easier sample preparation method, decreased analysis time and reduces the need for complicated or expensive equipment. The sample preparation method used in this study can also be further extended to higherend analytical techniques and other biological samples for quantification of bosentan.

scholarly journals Validation of a simple sample preparation method for multielement analysis of bovine serum

PLoS ONE ◽  
2019 ◽  
Vol 14 (2) ◽  
pp. e0211859 ◽  
Author(s):  
Diego Luna ◽  
Marta Miranda ◽  
Antonio Humberto Hamad Minervino ◽  
Verónica Piñeiro ◽  
Carlos Herrero-Latorre ◽  
...  
Keyword(s):  
Sample Preparation ◽  
Bovine Serum ◽  
Preparation Method ◽  
Multielement Analysis ◽  
Sample Preparation Method ◽  
Simple Sample Preparation

scholarly journals Immunoradiometric quantitation of tissue plasminogen activator-related antigen in human plasma: crypticity phenomenon and relationship to plasma fibrinolysis

Blood ◽  
1987 ◽  
Vol 69 (5) ◽  
pp. 1348-1353 ◽  
Author(s):  
TC Wun ◽  
A Capuano
Keyword(s):  
Sample Preparation ◽  
Human Plasma ◽  
Plasminogen Activator ◽  
Tissue Plasminogen Activator ◽  
Preparation Method ◽  
Blood Bank ◽  
Immunoradiometric Assay ◽  
Plasma Samples ◽  
Sample Preparation Method ◽  
Tissue Plasminogen

Abstract A two-site immunoradiometric assay for tissue plasminogen activator (tPA) antigen has been developed using immunoaffinity purified antibody. Various treatments enhanced the detection of tPA antigen in the plasma samples. Maximum detection was obtained by acidification of plasma to pH 4.8 to 6.5 or addition of 0.5 mol/L of L-lysine or L- arginine. Acidification or addition of lysine to plasma is also required for maximum immunoadsorption of plasma tPA antigen on anti-tPA- Ig-sepharose. These results indicate that plasma tPA antigen is partially cryptic to antibody in untreated plasma. The plasma tPA antigen isolated by immunoadsorption of either untreated plasma or acidified plasma on anti-tPA-Ig-sepharose consists mainly of a 100-kd plasminogen activator species as determined by fibrin-agar zymography. The 100-kd activity is possibly a tPA:inhibitor complex. A standardized sample preparation method was conveniently adopted by mixing 3 vol of plasma and 1 vol of 2 mol/L of L-lysine for the assay. Reconstitution and recovery studies showed that the method is specific and permits full detection of both free tPA and tPA:inhibitor complex. The validity of the assay is further supported by the finding that the spontaneous plasma fibrinolysis previously demonstrated to be dependent on plasma tPA antigen is correlated with tPA antigen content. Using the standardized assay, we found that tPA antigen concentrations in 16 blood bank plasmas are equivalent to 3.7 to 20 ng of 60 kd tPA/mL. In all the plasma tested, more than half of the antigen is undetected unless the plasma is treated as described above.

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