A high-throughput sample preparation method for cellular proteomics using 96-well filter plates

PROTEOMICS ◽  
2013 ◽  
Vol 13 (20) ◽  
pp. 2980-2983 ◽  
Author(s):  
Linda Switzar ◽  
Jordy van Angeren ◽  
Martijn Pinkse ◽  
Jeroen Kool ◽  
Wilfried M. A. Niessen
Keyword(s):  
Sample Preparation ◽  
High Throughput ◽  
Preparation Method ◽  
Sample Preparation Method

scholarly journals Sample preparation method to improve the efficiency of high-throughput single-molecule force spectroscopy

2019 ◽  
Vol 5 (4) ◽  
pp. 176-183 ◽  
Author(s):  
Lei Jin ◽  
Li Kou ◽  
Yanan Zeng ◽  
Chunguang Hu ◽  
Xiaodong Hu
Keyword(s):  
Sample Preparation ◽  
High Throughput ◽  
Single Molecule ◽  
Preparation Method ◽  
Force Spectroscopy ◽  
Experimental Result ◽  
Sample Preparation Method ◽  
Dna Stretching ◽  
Single Molecule Force Spectroscopy ◽  
Preparation Methods

Abstract Inefficient sample preparation methods hinder the performance of high-throughput single-molecule force spectroscopy (H-SMFS) for viscous damping among reactants and unstable linkage. Here, we demonstrated a sample preparation method for H-SMFS systems to achieve a higher ratio of effective target molecules per sample cell by gas-phase silanization and reactant hydrophobization. Digital holographic centrifugal force microscopy (DH-CFM) was used to verify its performance. The experimental result indicated that the DNA stretching success ratio was improved from 0.89% to 13.5%. This enhanced efficiency preparation method has potential application for force-based DNA stretching experiments and other modifying procedures.

An automated high-throughput sample preparation method using double-filtration for serum metabolite LC-MS analysis

2019 ◽  
Vol 11 (31) ◽  
pp. 4060-4065 ◽  
Author(s):  
Minjoong Joo ◽  
Jong-Moon Park ◽  
Van-An Duong ◽  
Dami Kwon ◽  
Jongho Jeon ◽  
...  
Keyword(s):  
Sample Preparation ◽  
High Throughput ◽  
Preparation Method ◽  
Sample Preparation Method ◽  
Ms Analysis ◽  
System P ◽  
Filtration System ◽  
Automated Sample Preparation ◽  
Double Filtration

A novel, automated sample preparation method for serum metabolite LC-MS analysis was developed using a double-filtration system.

scholarly journals Development of a 96-well plate sample preparation method for integrated N- and O-glycomics using porous graphitized carbon liquid chromatography-mass spectrometry

2020 ◽  
Vol 16 (4) ◽  
pp. 355-363 ◽  
Author(s):  
Tao Zhang ◽  
Katarina Madunić ◽  
Stephanie Holst ◽  
Jing Zhang ◽  
Chunsheng Jin ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Sample Preparation ◽  
High Throughput ◽  
Preparation Method ◽  
Liquid Chromatography Mass Spectrometry ◽  
Sample Preparation Method ◽  
Porous Graphitized Carbon ◽  
Graphitized Carbon ◽  
Chromatography Mass Spectrometry

The developed workflow allows high throughput sample preparation for glycomics analysis.

scholarly journals High-throughput quantification of carboxymethyl lysine in serum and plasma using high-resolution accurate mass Orbitrap mass spectrometry

2019 ◽  
Vol 56 (3) ◽  
pp. 397-407 ◽  
Author(s):  
Naomi J Rankin ◽  
Karl Burgess ◽  
Stefan Weidt ◽  
Goya Wannamethee ◽  
Naveed Sattar ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
High Resolution ◽  
Sample Preparation ◽  
High Throughput ◽  
Coefficient Of Variation ◽  
Preparation Method ◽  
Sample Preparation Method ◽  
Lysine Concentration ◽  
Carboxymethyl Lysine ◽  
High Resolution Accurate Mass

Background Carboxymethyl lysine is an advanced glycation end product of interest as a potential biomarker of cardiovascular and other diseases. Available methods involve ELISA, with potential interference, or isotope dilution mass spectrometry (IDMS), with low-throughput sample preparation. Methods A high-throughput sample preparation method based on 96-well plates was developed. Protein-bound carboxymethyl lysine and lysine were quantified by IDMS using reversed phase chromatography coupled to a high-resolution accurate mass Orbitrap Exactive mass spectrometer. The carboxymethyl lysine concentration (normalized to lysine concentration) was measured in 1714 plasma samples from the British Regional Heart Study (BRHS). Results For carboxymethyl lysine, the lower limit of quantification (LLOQ) was estimated at 0.16 μM and the assay was linear between 0.25 and 10 μM. For lysine, the LLOQ was estimated at 3.79 mM, and the assay was linear between 2.5 and 100 mM. The intra-assay coefficient of variation was 17.2% for carboxymethyl lysine, 9.3% for lysine and 10.5% for normalized carboxymethyl lysine. The inter-assay coefficient of variation was 18.1% for carboxymethyl lysine, 14.8 for lysine and 16.2% for normalized carboxymethyl lysine. The median and inter-quartile range of all study samples in each batch were monitored. A mean carboxymethyl lysine concentration of 2.7 μM (IQR 2.0–3.2 μM, range 0.2–17.4 μM) and a mean normalized carboxymethyl lysine concentration of 69 μM/M lysine (IQR 54–76 μM/M, range 19–453 μM/M) were measured in the BRHS. Conclusion This high-throughput sample preparation method makes it possible to analyse large cohorts required to determine the potential of carboxymethyl lysine as a biomarker.

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