Carbon Replica Technique for Examination of Paint Surfaces in Electron Microscopy

1957 ◽  
Vol 29 (5) ◽  
pp. 784-786 ◽  
Author(s):  
W. R. Lasko
Keyword(s):  
Electron Microscopy ◽  
Replica Technique ◽  
Carbon Replica

scholarly journals Studies of the Fine Structure of the Wax Layer of Sultana Grapes

1963 ◽  
Vol 16 (4) ◽  
pp. 818 ◽  
Author(s):  
TC Chambers ◽  
JV Possingham
Keyword(s):  
Electron Microscopy ◽  
Fine Structure ◽  
Drying Rate ◽  
Distilled Water ◽  
Replica Technique ◽  
Carbon Replica

The surface waxy bloom of sultana grapes has been studied using the carbon. replica technique in combination with electron microscopy. This layer consists of a series of overlapping wax platelets, each of which is about 0�1 f' wide. The air spaces between the wax platelets become filled with liquid when sultana berries are dipped in commercial emulsions known to accelerate their drying rate. Washing in distilled water removes this layer of dipping emulsion from the surface. The appearance of dipped and washed grapes is similar to that of untreated grapes.

scholarly journals The Structure of the Cuticular Wax of Prune Plums and its Influence as a Water Barrier

1967 ◽  
Vol 20 (5) ◽  
pp. 895 ◽  
Author(s):  
Joan M Bain ◽  
D Mcg Mcbean
Keyword(s):  
Electron Microscopy ◽  
Outer Layer ◽  
Layer Structure ◽  
Cuticular Wax ◽  
Replica Technique ◽  
Carbon Replica ◽  
Water Barrier

Wax on the surface of prune plums, sampled from 2 weeks before fruit was mature until 2 weeks after, was shown by electron microscopy, using the carbon� replica technique, to occur in a two-layer structure. The iruier layer consisted of a matrix of thin platelets, while the outer layer was composed of fragile projections, many of which appeared tubular. The incidence and complexity of the projections in the outer layer increased as the fruit matured.

Surface Structure of Nucleated Animal Cells: Carbon Replicas

1967 ◽  
Vol 25 ◽  
pp. 120-121
Author(s):  
Robert M. Glaeser ◽  
Thea B. Scott
Keyword(s):  
Cell Surface ◽  
Surface Structure ◽  
Particle Diameter ◽  
Cellular Functions ◽  
Animal Cells ◽  
Replica Technique ◽  
Carbon Replica ◽  
Characteristic Particle ◽  
Cell Surface Structure ◽  
Carbon Replicas

The carbon-replica technique can be used to obtain information about cell-surface structure that cannot ordinarily be obtained by thin-section techniques. Mammalian erythrocytes have been studied by the replica technique and they appear to be characterized by a pebbly or “plaqued“ surface texture. The characteristic “particle” diameter is about 200 Å to 400 Å. We have now extended our observations on cell-surface structure to chicken and frog erythrocytes, which possess a broad range of cellular functions, and to normal rat lymphocytes and mouse ascites tumor cells, which are capable of cell division. In these experiments fresh cells were washed in Eagle's Minimum Essential Medium Salt Solution (for suspension cultures) and one volume of a 10% cell suspension was added to one volume of 2% OsO4 or 5% gluteraldehyde in 0.067 M phosphate buffer, pH 7.3. Carbon replicas were obtained by a technique similar to that employed by Glaeser et al. Figure 1 shows an electron micrograph of a carbon replica made from a chicken erythrocyte, and Figure 2 shows an enlarged portion of the same cell.

Studies of Circular DNA Using a New Electron Microscopic Technique

1973 ◽  
Vol 31 ◽  
pp. 596-597
Author(s):  
C. N. Gordon
Keyword(s):  
Electron Microscopy ◽  
Aqueous Salt Solution ◽  
Contour Length ◽  
Cleavage Surface ◽  
Mica Substrate ◽  
Electron Microscopic ◽  
Replica Technique ◽  
Circular Dna ◽  
Transmission Electron ◽  
Electron Microscopic Technique

Gordon and Kleinschmidt have described a new preparative technique for visualizing DNA by electron microscopy. This procedure, which is a modification of Hall's “mica substrate technique”, consists of the following steps: (a) K+ ions on the cleavage surface of native mica are exchanged for Al3+ ions by ion exchange. (b) The mica, with Al3+ in the exchange sites on the surface, is placed in a dilute aqueous salt solution of DNA for several minutes; during this period DNA becomes adsorbed on the surface. (c) The mica with adsorbed DNA is removed from the DNA solution, rinsed, dried and visualized for transmission electron microscopy by Hall's platinum pre-shadow replica technique.In previous studies of circular DNA by this technique, most of the molecules seen were either broken to linears or extensively tangled; in general, it was not possible to obtain suitably large samples of open extended molecules for contour length measurements.

A Positive‐Replica Technique for Electron Microscopy

1949 ◽  
Vol 20 (2) ◽  
pp. 202-205 ◽  
Author(s):  
C. M. Schwartz ◽  
A. E. Austin ◽  
P. M. Weber
Keyword(s):  
Electron Microscopy ◽  
Replica Technique

scholarly journals Seeing is believing? When scanning electron microscopy (SEM) meets clinical dentistry: The replica technique.

2020 ◽  
Vol 83 (9) ◽  
pp. 1118-1123
Author(s):  
Lucas Zago Naves ◽  
David‐Alain Gerdolle ◽  
Oswaldo Scopin Andrade ◽  
Marco Gresnigt
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Replica Technique ◽  
Scanning Electron

Direct Imaging of Sodium Stearate Crystals Dispersed in Waterpropylene Glycol Mixtures by Cryo-Electron Microscopy

2001 ◽  
Vol 7 (S2) ◽  
pp. 734-735
Author(s):  
Yue Ma ◽  
J. Liang ◽  
Y. Zheng ◽  
S. L. Erlandsen ◽  
L. E. Scriven ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Practical Importance ◽  
Sodium Stearate ◽  
Original Structure ◽  
Replica Technique ◽  
X Ray Scattering ◽  
Cryo Electron Microscopy ◽  
Transmission Electron ◽  
Surface Active

Cryo-scanning electron microscopy (cryo-SEM) and cryo-transmission electron microscopy (cryo- TEM), in conjunctions with rheological measurements, light and confocal microscopy, x-ray scattering, and solid state NMR, are used to characterize sodium stearate (NaSt) crystals dispersed in waterpropylene glycol (PG) mixtures at macroscopic, microscopic, molecular, and atomic levels. NaSt is a surface-active, structural agent in household and personal cleaning products, including deodorant sticks and soap bars. A better structural characterization of NaSt/PG/water systems has practical importance in personal care and cosmetic industries. NaSt crystals and other soap crystal morphologies have been studied by the TEM/replica technique. However, the replicas were made of the residue after the original sample or its aqueous dilution were dried, and the original structure may have been lost during drying. Cryo-SEM was not used to study NaSt crystals because of its lower resolution and because the crystals are highly susceptible to radiation damage by electron beam.

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