A Positive‐Replica Technique for Electron Microscopy

1949 ◽  
Vol 20 (2) ◽  
pp. 202-205 ◽  
Author(s):  
C. M. Schwartz ◽  
A. E. Austin ◽  
P. M. Weber
Keyword(s):  
Electron Microscopy ◽  
Replica Technique

Studies of Circular DNA Using a New Electron Microscopic Technique

1973 ◽  
Vol 31 ◽  
pp. 596-597
Author(s):  
C. N. Gordon
Keyword(s):  
Electron Microscopy ◽  
Aqueous Salt Solution ◽  
Contour Length ◽  
Cleavage Surface ◽  
Mica Substrate ◽  
Electron Microscopic ◽  
Replica Technique ◽  
Circular Dna ◽  
Transmission Electron ◽  
Electron Microscopic Technique

Gordon and Kleinschmidt have described a new preparative technique for visualizing DNA by electron microscopy. This procedure, which is a modification of Hall's “mica substrate technique”, consists of the following steps: (a) K+ ions on the cleavage surface of native mica are exchanged for Al3+ ions by ion exchange. (b) The mica, with Al3+ in the exchange sites on the surface, is placed in a dilute aqueous salt solution of DNA for several minutes; during this period DNA becomes adsorbed on the surface. (c) The mica with adsorbed DNA is removed from the DNA solution, rinsed, dried and visualized for transmission electron microscopy by Hall's platinum pre-shadow replica technique.In previous studies of circular DNA by this technique, most of the molecules seen were either broken to linears or extensively tangled; in general, it was not possible to obtain suitably large samples of open extended molecules for contour length measurements.

scholarly journals Seeing is believing? When scanning electron microscopy (SEM) meets clinical dentistry: The replica technique.

2020 ◽  
Vol 83 (9) ◽  
pp. 1118-1123
Author(s):  
Lucas Zago Naves ◽  
David‐Alain Gerdolle ◽  
Oswaldo Scopin Andrade ◽  
Marco Gresnigt
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Replica Technique ◽  
Scanning Electron

Direct Imaging of Sodium Stearate Crystals Dispersed in Waterpropylene Glycol Mixtures by Cryo-Electron Microscopy

2001 ◽  
Vol 7 (S2) ◽  
pp. 734-735
Author(s):  
Yue Ma ◽  
J. Liang ◽  
Y. Zheng ◽  
S. L. Erlandsen ◽  
L. E. Scriven ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Practical Importance ◽  
Sodium Stearate ◽  
Original Structure ◽  
Replica Technique ◽  
X Ray Scattering ◽  
Cryo Electron Microscopy ◽  
Transmission Electron ◽  
Surface Active

Cryo-scanning electron microscopy (cryo-SEM) and cryo-transmission electron microscopy (cryo- TEM), in conjunctions with rheological measurements, light and confocal microscopy, x-ray scattering, and solid state NMR, are used to characterize sodium stearate (NaSt) crystals dispersed in waterpropylene glycol (PG) mixtures at macroscopic, microscopic, molecular, and atomic levels. NaSt is a surface-active, structural agent in household and personal cleaning products, including deodorant sticks and soap bars. A better structural characterization of NaSt/PG/water systems has practical importance in personal care and cosmetic industries. NaSt crystals and other soap crystal morphologies have been studied by the TEM/replica technique. However, the replicas were made of the residue after the original sample or its aqueous dilution were dried, and the original structure may have been lost during drying. Cryo-SEM was not used to study NaSt crystals because of its lower resolution and because the crystals are highly susceptible to radiation damage by electron beam.

Ultrastructural Studies Relating to the Surface Morphology of Cultured Cells

1970 ◽  
Vol 6 (2) ◽  
pp. 477-484
Author(s):  
R. G. P. PUGH-HUMPHREYS ◽  
W. SINCLAIR
Keyword(s):  
Electron Microscopy ◽  
Cultured Cells ◽  
Kidney Cells ◽  
Replica Technique ◽  
Regular Feature ◽  
Ultrastructural Studies ◽  
Transmission Electron ◽  
Surface Replica ◽  
Mesenchyme Cells ◽  
Canine Kidney

Scanning electron microscopy and a surface-replica technique for transmission electron microscopy have been used to study the ultrastructural features of cultured-cell surfaces The presence of microvilli measuring 0.1-0.2 µm in diameter by up to 5 µm in length has been noted as a regular feature of Landschütz ascites, ‘fibroblastic’ HeLa, and canine kidney, cells. The surfaces of chick mesenchyme cells were notably almost devoid of microvilli. The presence of microvilli at the cell surface is discussed briefly.

Modification of a Positive‐Replica Technique for Electron Microscopy

1951 ◽  
Vol 22 (6) ◽  
pp. 847-848 ◽  
Author(s):  
A. E. Austin ◽  
C. M. Schwartz
Keyword(s):  
Electron Microscopy ◽  
Replica Technique
Sign in / Sign up

Export Citation Format

Share Document