scholarly journals Seeing is believing? When scanning electron microscopy (SEM) meets clinical dentistry: The replica technique.

2020 ◽  
Vol 83 (9) ◽  
pp. 1118-1123
Author(s):  
Lucas Zago Naves ◽  
David‐Alain Gerdolle ◽  
Oswaldo Scopin Andrade ◽  
Marco Gresnigt
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Replica Technique ◽  
Scanning Electron

A metallic replica technique for scanning electron microscopy

1973 ◽  
Vol 8 (11) ◽  
pp. 1670-1672 ◽  
Author(s):  
W. Wu ◽  
A. S. Argon ◽  
A. P. L. Turner
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Replica Technique ◽  
Scanning Electron

Fine structure of normal and regenerated shell of Helix

1971 ◽  
Vol 49 (1) ◽  
pp. 37-41 ◽  
Author(s):  
A. S. M. Saleuddin
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Fine Structure ◽  
Organic Matrix ◽  
Nacreous Layer ◽  
Replica Technique ◽  
Thin Sections ◽  
Thin Sectioning ◽  
Inorganic Components ◽  
Scanning Electron

Fine structure of the normal and the regenerated shell of Helix has been studied by thin sectioning, replica technique, and scanning electron microscopy. Normal shell consists of four calcareous layers: innermost nacreous, two cross lamellar, and outermost prismatic. Crystals of the shell are well defined and are surrounded by intercrystalline organic matrix. Intracrystalline organic matrix is recognized, particularly in decalcified sections. Interrelationships between the organic and inorganic components have been studied in decalcified thin sections. Regenerated shell appears similar to nacreous layer of the normal shell. Crystals are large and stacked like bricks. Intracrystalline organic matrix is very prominent. Electron diffraction of the crystals of the regenerated shell generally gives calcite pattern whereas the normal shell gives aragonite. Surface topography of the normal and regenerated shell has been compared by replica techniques.

Pathogenesis of Human Hair Defects

1974 ◽  
Vol 32 ◽  
pp. 12-13
Author(s):  
P.S. Porter ◽  
T. Aoyagi ◽  
R. Matta
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Human Hair ◽  
Standard Techniques ◽  
Scanning Electron

Using standard techniques of scanning electron microscopy (SEM), over 1000 human hair defects have been studied. In several of the defects, the pathogenesis of the abnormality has been clarified using these techniques. It is the purpose of this paper to present several distinct morphologic abnormalities of hair and to discuss their pathogenesis as elucidated through techniques of scanning electron microscopy.

Ultrastructural Analysis of the Salivary Glands of Gromphadorhina Portentosa (Schaum)

1977 ◽  
Vol 35 ◽  
pp. 638-639
Author(s):  
P.J. Dailey
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Salivary Glands ◽  
Secretory Vesicles ◽  
The Past ◽  
Transmission Electron ◽  
Means Of Transmission ◽  
Gromphadorhina Portentosa ◽  
Scanning Electron ◽  
Topographical Relief

The structure of insect salivary glands has been extensively investigated during the past decade; however, none have attempted scanning electron microscopy (SEM) in ultrastructural examinations of these secretory organs. This study correlates fine structure by means of SEM cryofractography with that of thin-sectioned epoxy embedded material observed by means of transmission electron microscopy (TEM).Salivary glands of Gromphadorhina portentosa were excised and immediately submerged in cold (4°C) paraformaldehyde-glutaraldehyde fixative1 for 2 hr, washed and post-fixed in 1 per cent 0s04 in phosphosphate buffer (4°C for 2 hr). After ethanolic dehydration half of the samples were embedded in Epon 812 for TEM and half cryofractured and subsequently critical point dried for SEM. Dried specimens were mounted on aluminum stubs and coated with approximately 150 Å of gold in a cold sputtering apparatus.Figure 1 shows a cryofractured plane through a salivary acinus revealing topographical relief of secretory vesicles.

Two- or three-way observations of the identical cell elements within the same sections by LM, TEM and/or SEM

1978 ◽  
Vol 36 (2) ◽  
pp. 82-83 ◽  
Author(s):  
Nakazo Watari ◽  
Yasuaki Hotta ◽  
Yoshio Mabuchi
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Fine Structure ◽  
Light Microscopy ◽  
Thin Sections ◽  
Transmission Electron ◽  
Identical Cell ◽  
Electron Microscopes ◽  
Scanning Electron

It is very useful if we can observe the identical cell elements within the same sections by light microscopy (LM), transmission electron microscopy (TEM) and/or scanning electron microscopy (SEM) sequentially, because, the cell fine structure can not be indicated by LM, while the color is; on the other hand, the cell fine structure can be very easily observed by EM, although its color properties may not. However, there is one problem in that LM requires thick sections of over 1 μm, while EM needs very thin sections of under 100 nm. Recently, we have developed a new method to observe the same cell elements within the same plastic sections using both light and transmission (conventional or high-voltage) electron microscopes.In this paper, we have developed two new observation methods for the identical cell elements within the same sections, both plastic-embedded and paraffin-embedded, using light microscopy, transmission electron microscopy and/or scanning electron microscopy (Fig. 1).

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