Quantitative Determination of Enzyme Activity in Single Cells by Scanning Microelectrode Coupled with a Nitrocellulose Film-Covered Microreactor by Means of a Scanning Electrochemical Microscope

2007 ◽  
Vol 79 (3) ◽  
pp. 1256-1261 ◽  
Author(s):  
Xiaoli Zhang ◽  
Fuchan Sun ◽  
Xuewei Peng ◽  
Wenrui Jin
Keyword(s):  
Enzyme Activity ◽  
Quantitative Determination ◽  
Single Cells ◽  
Scanning Electrochemical Microscope

A Rapid Electrophoretic Method for the Determination of the Isoenzymes of Serum Lactate Dehydrogenase

1966 ◽  
Vol 12 (5) ◽  
pp. 308-313 ◽  
Author(s):  
Albert W Opher ◽  
Charles S Collier ◽  
Joseph M Miller
Keyword(s):  
Enzyme Activity ◽  
Lactate Dehydrogenase ◽  
Quantitative Determination ◽  
Serum Lactate ◽  
Serum Lactate Dehydrogenase ◽  
Electrophoretic Method ◽  
The Individual ◽  
Electrophoretic Procedure ◽  
Purple Formazan

Abstract A convenient electrophoretic procedure for the separation and quantitation of lactate dehydrogenase (LDH) isoenzymes is described. The system uses polyacetate Sepraphore III strips.* The areas of activity are shown by incubation with an LDH substrate combined with tetra-nitro-blue-tetrazolium. The reduction of the latter to the purple formazan is quantitatively related to the enzyme activity. Quantitative determination of the individual colored areas is performed by densitometry.

scholarly journals EXTRACTION, HYDROLYSIS, AND ELECTROPHORETIC ANALYSIS OF RIBONUCLEIC ACID FROM MICROSCOPIC TISSUE UNITS (MICROPHORESIS)

1960 ◽  
Vol 8 (1) ◽  
pp. 39-46 ◽  
Author(s):  
Jan-Erik Edström
Keyword(s):  
Quantitative Determination ◽  
Ultraviolet Light ◽  
Coefficient Of Variation ◽  
Ribonucleic Acid ◽  
Single Cells ◽  
Electrophoretic Analysis ◽  
Test Substance ◽  
Different Sources ◽  
Purine Pyrimidine

A procedure for the purine-pyrimidine analysis of RNA in the 100- to 1000 µµg. range is presented. It includes hydrolysis and electrophoretic analysis of RNA, which is extracted from single isolated tissue units, like single cells. The quantitative determination of the separated compounds is carried out by a photographic-photometric procedure in ultraviolet light. The determined values show a coefficient of variation of about ±7 per cent on test substance. Microelectrophoretic analyses of RNA from different sources have been performed and are compared to macrochemical analyses. The agreement is good in those cases in which it is possible to get any information at all through macrochemical analyses.

scholarly journals Direct quantitative determination of ceramide glycosylation in vivo: a new approach to evaluate cellular enzyme activity of glucosylceramide synthase

2009 ◽  
Vol 51 (4) ◽  
pp. 866-874 ◽  
Author(s):  
Vineet Gupta ◽  
Gauri A. Patwardhan ◽  
Qian-Jin Zhang ◽  
Myles C. Cabot ◽  
S. Michal Jazwinski ◽  
...  
Keyword(s):  
Enzyme Activity ◽  
Quantitative Determination ◽  
Glucosylceramide Synthase ◽  
New Approach

QUANTITATIVE DETERMINATION OF ENZYME ACTIVITY IN DUODENAL FLUIDS

1923 ◽  
Vol 166 (4) ◽  
pp. 535-538 ◽  
Author(s):  
C. W. Lueders ◽  
Olaf Bergeim ◽  
Martin E. Rehfuss
Keyword(s):  
Enzyme Activity ◽  
Quantitative Determination

MICRO-PIXE FOR SINGLE CELL ANALYSIS

2012 ◽  
Vol 22 (01n02) ◽  
pp. 51-56 ◽  
Author(s):  
RICHARD ORTEGA
Keyword(s):  
Trace Elements ◽  
Quantitative Determination ◽  
Single Cell Analysis ◽  
Direct Detection ◽  
Single Cells ◽  
Trace Element Content ◽  
Scanning Transmission ◽  
Resolution Imaging ◽  
Cellular Compartments

The knowledge of the intracellular distribution of biological relevant metals is important to understand their mechanisms of action in cells, either for physiological, toxicological or pathological processes. However, the direct detection of trace metals in single cells is a challenging task that requires sophisticated analytical developments. The combination of micro-PIXE with RBS and STIM (Scanning Transmission Ion Microscopy) allows the quantitative determination of trace metal content within sub-cellular compartments. The application of STIM analysis provides high spatial resolution imaging (< 200 nm) and excellent mass sensitivity (< 0.1 ng). Application of the STIM-PIXE-RBS methodology is absolutely needed when organic mass loss appears during PIXE-RBS irradiation. This combination of STIM-PIXE-RBS provides fully quantitative determination of trace element content, expressed in μg/g, which is a quite unique capability for micro-PIXE compared to other micro-analytical methods such as the electron and synchrotron x-ray fluorescence. Examples of micro-PIXE studies for sub-cellular imaging of trace elements in various fields of interest will be presented: in patho-physiology of trace elements involved in neurodegenerative diseases such as Parkinson's disease, and in toxicology of metals such as cobalt.

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