Quantitative Determination of the Pigment Content of Single Cells by Means of a New Microspectrophotometer

1948 ◽  
Vol 35 (3) ◽  
pp. 239 ◽  
Author(s):  
Barry Commoner
Keyword(s):  
Quantitative Determination ◽  
Single Cells ◽  
Pigment Content

scholarly journals EXTRACTION, HYDROLYSIS, AND ELECTROPHORETIC ANALYSIS OF RIBONUCLEIC ACID FROM MICROSCOPIC TISSUE UNITS (MICROPHORESIS)

1960 ◽  
Vol 8 (1) ◽  
pp. 39-46 ◽  
Author(s):  
Jan-Erik Edström
Keyword(s):  
Quantitative Determination ◽  
Ultraviolet Light ◽  
Coefficient Of Variation ◽  
Ribonucleic Acid ◽  
Single Cells ◽  
Electrophoretic Analysis ◽  
Test Substance ◽  
Different Sources ◽  
Purine Pyrimidine

A procedure for the purine-pyrimidine analysis of RNA in the 100- to 1000 µµg. range is presented. It includes hydrolysis and electrophoretic analysis of RNA, which is extracted from single isolated tissue units, like single cells. The quantitative determination of the separated compounds is carried out by a photographic-photometric procedure in ultraviolet light. The determined values show a coefficient of variation of about ±7 per cent on test substance. Microelectrophoretic analyses of RNA from different sources have been performed and are compared to macrochemical analyses. The agreement is good in those cases in which it is possible to get any information at all through macrochemical analyses.

MICRO-PIXE FOR SINGLE CELL ANALYSIS

2012 ◽  
Vol 22 (01n02) ◽  
pp. 51-56 ◽  
Author(s):  
RICHARD ORTEGA
Keyword(s):  
Trace Elements ◽  
Quantitative Determination ◽  
Single Cell Analysis ◽  
Direct Detection ◽  
Single Cells ◽  
Trace Element Content ◽  
Scanning Transmission ◽  
Resolution Imaging ◽  
Cellular Compartments

The knowledge of the intracellular distribution of biological relevant metals is important to understand their mechanisms of action in cells, either for physiological, toxicological or pathological processes. However, the direct detection of trace metals in single cells is a challenging task that requires sophisticated analytical developments. The combination of micro-PIXE with RBS and STIM (Scanning Transmission Ion Microscopy) allows the quantitative determination of trace metal content within sub-cellular compartments. The application of STIM analysis provides high spatial resolution imaging (< 200 nm) and excellent mass sensitivity (< 0.1 ng). Application of the STIM-PIXE-RBS methodology is absolutely needed when organic mass loss appears during PIXE-RBS irradiation. This combination of STIM-PIXE-RBS provides fully quantitative determination of trace element content, expressed in μg/g, which is a quite unique capability for micro-PIXE compared to other micro-analytical methods such as the electron and synchrotron x-ray fluorescence. Examples of micro-PIXE studies for sub-cellular imaging of trace elements in various fields of interest will be presented: in patho-physiology of trace elements involved in neurodegenerative diseases such as Parkinson's disease, and in toxicology of metals such as cobalt.

Quantitative determination of longitudinal and transverse cross-relaxation rates

1999 ◽  
Vol 96 (9/10) ◽  
pp. 1608-1615
Author(s):  
T. E. Malliavin ◽  
H. Desvaux ◽  
M. A. Delsuc
Keyword(s):  
Quantitative Determination ◽  
Cross Relaxation ◽  
Relaxation Rates

Quantitative Determination of Gallic acid and Cyanidin-3-O-Glucoside within Sumac Extracts by HPLC-MS/MS

Planta Medica ◽  
2011 ◽  
Vol 77 (12) ◽  
Author(s):  
M Koşar ◽  
F Göger ◽  
N Kırımer ◽  
KHC Başer
Keyword(s):  
Quantitative Determination ◽  
Gallic Acid

Quantitative determination of compound leads in Schiniopsis brasiliensis Engler

Planta Medica ◽  
2014 ◽  
Vol 80 (16) ◽  
Author(s):  
FHA Fernandes ◽  
RSA Batista ◽  
G Véras ◽  
FS Souza ◽  
ACD Medeiros
Keyword(s):  
Quantitative Determination
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